Evaluation of Promastigote and Amastigote Antigens in the Indirect Fluorescent Antibody Test for American Cutaneous Leishmaniasis *

Pappas, Michael G.; McGreevy, P. B.; Hajkowski, Ruta; Hendricks, Larry D.; Oster, Charles N.; Hockmeyer, Wayne T.

doi:10.4269/ajtmh.1983.32.1260

Cited by 41 publications

(17 citation statements)

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“…The overall cross-reactivity observed in serum samples from patients with positive diagnosis for ATL and in non-related microorganisms and other conditions indicates antigenic similarity between Leishmania and bacteria such as Mycobacterium species, as demonstrated by significant cross-reactivity in serologic tests. 51,52,59,60,62 We did not find any common antigenic band with 100% recognition that was diagnostic for all sera from patients with ATL. The more specific fractions for Leishmania antigens were 42 kD for L. (V.) braziliensis, 50 kD for L. (V.) amazonensis, and 58 kD for L. (L.) tropica with a frequency of recognition of 53.70%, 72%, and 47.80%, respectively.…”

Section: Discussionmentioning

confidence: 94%

“…This could be explained in part by the complexity of the antigenic constitution of each parasite and by the genetic background of each individual, which together influence the immune response. 22,51 Western blotting showed that serum samples from patients with ATL recognized 31 bands (13- 31 A comparison of the MW r values of protein fractions is a complex process, and many factors such as composition of the culture media, parasite growth phase, concentration of SDS and 2-mercaptoethanol in the sample buffer, separation of soluble fractions from pellets, SDS-PAGE gel size, polyacrylamide concentration, and the molecular weight markers used can influence the fraction migration and determination of the MW r values. 34 Thus, direct comparisons of molecular weights of antigens cited in the literature are always very difficult and must be done with caution.…”

Section: Discussionmentioning

confidence: 99%

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Evaluation of antigens from various Leishmania species in a Western blot for diagnosis of American tegumentary leishmaniasis.

Gonçalves¹,

Reiche²,

Filho³

et al. 2002

The American Journal of Tropical Medicine and Hygiene

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Abstract. A Western blot method that uses antigens from culture promastigote forms of Leishmania (Viannia) braziliensis, L. (Leishmania) amazonensis, L. (Leishmania) tropica, and a trypanosomatid (strain 268T) isolated from naturally infected tomatoes was evaluated for laboratory diagnosis of American tegumentary leishmaniasis (ATL). Serum samples were obtained from 108 patients with ATL (group I), 23 chagasic patients (group II), 32 patients with other diseases (group III), and 78 healthy individuals (group IV). The overall analysis showed a sensitivity of 76.90%, 90.40%, 78.50%, and 87.90%, a specificity of 100%, 93.80%, 87.80%, and 77.10%, a positive predictive value of 100%, 94.00%, 89.50%, and 72.50%, a negative predictive value of 75.70%, 90.00%, 75.40%, and 90.20%, and a concordance coefficient kappa of 0.7358, 0.8400, 0.6491, and 0.6287 for L.(L.) tropica, and strain 268T antigens, respectively. The antigenic profile recognized by serum samples from patients with ATL and with Chagas' disease permits serologic distinction between these infections.

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Section: Discussionmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 99%

Evaluation of antigens from various Leishmania species in a Western blot for diagnosis of American tegumentary leishmaniasis.

Gonçalves¹,

Reiche²,

Filho³

et al. 2002

The American Journal of Tropical Medicine and Hygiene

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“…Variations in sensitivity and specificity of the IFAT in diagnosing some human leishmaniases, depending on the life cycle stage (amastigotes, promastigotes), have been reported. 10,11 Our results showed that the IFATa is equally specific and more sensitive than the currently used IFATp in detecting canine leishmaniasis by L. infantum.…”

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confidence: 73%

“…Some diagnostic methods, such as an ELISA 4,5 or Western blotting are more sensitive, 6,7 but an indirect immunofluorescence test (IFAT) has been found to be suitable for follow-up of recovery after chemotherapy, 8 and is by far the test most accepted by veterinary practitioners. Leishmania amastigotes have been identified in canine tissues by immunoperoxidase staining 9 and amastigotes of some human Leishmania species have been used in IFAT, 10,11 but to our knowledge, there is no information on L. infantum.…”

mentioning

confidence: 99%

Short report: improved diagnosis and follow-up of canine leishmaniasis using amastigote-based indirect immunofluorescence.

Fernández-Pérez

Méndez²,

Fuente³

et al. 1999

The American Journal of Tropical Medicine and Hygiene

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Abstract. A comparison of an indirect immunofluorescence test using promastigotes (IFATp) or cultured amastigotes (IFATa) in the diagnosis and follow-up of canine leishmaniasis caused by Leishmania infantum was carried out. Results obtained with both diagnostic methods were in good agreement although the IFATa titration was more sensitive than the currently used IFATp without losing specificity. The higher sensitivity of the amastigote-based IFAT resulted in an earlier diagnosis in the absence of clinical signs. Both methods showed comparable results for monitoring the clinical evolution of naturally infected and treated (meglumine antimoniate plus allopurinol) dogs.

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“…from a patient with American visce ral leishmaniasis proceeding from the locality o f Chuao, Aragua Sate (25 km from Guayabita), according to the technique described by Pappas et al (1983). A reaction was considered positive w hen more than 5 0 % o f the organisms show ed com plete per ipheral fluorescence (titers≥ 1:32).…”

Section: Parasitological and Serological Methodsmentioning

confidence: 99%

The re-emergence of American visceral leishmaniasis in an old focus in Venezuela: present situation of human and canine infections

Delgado

Feliciangeli

et al. 1998

Parasite

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Summary :The report of a new autochthonous case of human American Visceral Leishmaniasis (AVL) in 1992 in the village of Guayabita, Aragua State, Venezuela ( 10° 16' N, 6 7°2 8 'W ; 5 0 0 m asl), led us to undertake an epidemiological study in this locality. A demographic survey was conducted using a structured questionnaire in which data was collected regarding sex, age, occupation, length of residence in the area and migratory history. A leishmanin skin test (LST) was applied and samples of venous blood for counter immunoelectrophoresis (CIEP) and immunofluorescent antibodies (IFAT) tests were drawn. The prevalence of positive LST was 11.4 %. The positivity was highest among males and increased with age. Young males seemed to be more exposed to infection than females. Since occupation per se did not seem to account for this association, it may be explained as a gender-associated behavior, leading to different degrees of exposure to sand flies. The canine population was also screened for leishmanial infection. One dog was shown to be parasitologically infected with Leishmania sp. Four out of 71 dogs (5.6 %) were positive for FG, CIEP, IFAT and Western Blot and 11 (15.5 %) were positive for CIEP. These results suggest that after almost 30 years of epidemiological silence, American visceral leishmaniasis has re-emerged in this focus.KEY WORDS : AVL, human visce ral leishmaniasis, canine visce ral leishmaniasis, re-emergence, Venezuela.R é s u m é : R é -é m e r g e n c e d e la le ish m a n io se v isc éra le a m ér ica in e da n s UN FOYER ANCIEN DU CENTRE-NORD

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Evaluation of Promastigote and Amastigote Antigens in the Indirect Fluorescent Antibody Test for American Cutaneous Leishmaniasis *

Cited by 41 publications

References 0 publications

Evaluation of antigens from various Leishmania species in a Western blot for diagnosis of American tegumentary leishmaniasis.

Evaluation of antigens from various Leishmania species in a Western blot for diagnosis of American tegumentary leishmaniasis.

Short report: improved diagnosis and follow-up of canine leishmaniasis using amastigote-based indirect immunofluorescence.

The re-emergence of American visceral leishmaniasis in an old focus in Venezuela: present situation of human and canine infections

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