2014
DOI: 10.1002/jcla.21694
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Evaluation of Protease Inhibitors Containing Tubes for MS-Based Plasma Peptide Profiling Studies

Abstract: Considering that time taken to process blood samples affects their peptide profile and a decrease in PI's effect occurs over time, it may be concluded that the use of tubes containing PI for blood collection may be advantageous in the context of research, but may have some limitations regarding clinical practice.

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Cited by 5 publications
(6 citation statements)
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“…Accordingly, standardization of sample collection and processing is necessary to avoid any bias related to insufficient sample quality that might lead to misinterpretation of data . Alternatively, stabilizing procedures include the addition of protease inhibitors and thermal treatment . In nonstabilized blood specimens the time‐dependent decay pattern of endogenous peptides can be used for preanalytical quality control (QC) monitoring .…”
Section: Critical Steps For Ms‐based Protein Quantificationmentioning
confidence: 99%
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“…Accordingly, standardization of sample collection and processing is necessary to avoid any bias related to insufficient sample quality that might lead to misinterpretation of data . Alternatively, stabilizing procedures include the addition of protease inhibitors and thermal treatment . In nonstabilized blood specimens the time‐dependent decay pattern of endogenous peptides can be used for preanalytical quality control (QC) monitoring .…”
Section: Critical Steps For Ms‐based Protein Quantificationmentioning
confidence: 99%
“…Fortunately, the relative lack of specificity of most antibodies to minor changes in protein sequence or the presence of PTMs also offer an opportunity for collectively enriching an entire range of isoforms of a particular disease‐related protein of interest. This enrichment strategy coupled with specific detection at the level of amino acid sequence provided by the MS might foster a next‐generation of highly specific assay for the analysis of clinically relevant protein isoforms .…”
Section: Critical Steps For Ms‐based Protein Quantificationmentioning
confidence: 99%
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“…To date, only a few studies have investigated to some extent the influence of some blood collection parameters and the effects of long-term human plasma storage on peptide stability. However, they investigated only one specific peptide and/or one specific factor. None of the reported studies investigated simultaneously the three critical variables in blood collection (temperature, choice of stabilizing additive, and time) on a broad range of peptides.…”
Section: Introductionmentioning
confidence: 99%
“…In addition, various tools were developed that use previous experimental data, peptide amino acidic composition, and their physiochemical properties to derive suitability scores and thus suggest the best candidate peptides for quantitative proteomics. , Beyond the effect of tryptic digestion and missed cleavages, short- and midterm peptide stability can have a profound effect on quantification, especially in large proteomics experiments that expand for several days or weeks. Despite its importance in other fields, to date only few studies have investigated peptide stability, either in the context of handling and storage of liquid biopsies, , or in the development of specific targeted proteomics assays. Here, we expanded this knowledge by assessing the stability of the human proteotypic peptides under autosampler conditions for a period of 21 days, and we used this knowledge to train a deep learning model that predicts the stability for new tryptic peptide sequences.…”
mentioning
confidence: 99%