Evaluation of Protective Effects of Trehalose on Desiccation of Epithelial Cells in Three Dimensional Reconstructed Human Corneal Epithelium

Hovakimyan, Marina; Ramoth, Theresa; Löbler, Marian; Schmitz, Klaus‐Peter; Witt, Martin; Guthoff, Rudolf; Stachs, Oliver

doi:10.3109/02713683.2012.700754

Cited by 31 publications

(20 citation statements)

References 18 publications

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“…The mechanisms of TH in protecting corneal epithelial cells have been reported in some details (Izawa et al 2006;Luyckx & Baudouin 2011;Hovakimyan et al 2012;Hill-Bator et al 2014) and include water replacement in the desiccated cells by hydrogen bonding interactions with polar groups on membrane lipids and proteins (Leidy et al 2004), modification of the tetrahedral hydrogen-bond structure of water and rearrangement of the remaining water molecules around biological structures (Teramoto et al 2008) and reduced tendency to form crystals that can damage cell organelles (Luyckx & Baudouin 2011). These bioprotective effects of TH have also been proven in a number of clinical studies (Matsuo 2004;Ramoth et al 2013;Pinto-Bonilla et al 2015).…”

Section: Discussionmentioning

confidence: 99%

Effect of different lubricant eye gels on tear film thickness as measured with ultrahigh‐resolution optical coherence tomography

Woźniak

Schmidl

Bata

et al. 2016

Acta Ophthalmologica

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Section: Discussionmentioning

confidence: 99%

Effect of different lubricant eye gels on tear film thickness as measured with ultrahigh‐resolution optical coherence tomography

Woźniak

Schmidl

Bata

et al. 2016

Acta Ophthalmologica

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“…Trehalose-containing eyedrops have previously been shown to be effective in in vitro, 9 , 10 ex vivo, 11 and in vivo animal studies 12 as well as in a few clinical studies. 13 – 15 A further advantage of this formulation is a longer residency time than hyaluronate alone, as recently shown in a randomized, double-masked study.…”

Section: Discussionmentioning

confidence: 99%

A randomized crossover study comparing trehalose/hyaluronate eyedrops and standard treatment: patient satisfaction in the treatment of dry eye syndrome

Pinto-Bonilla¹,

Olmo-Jimeno²,

Llovet‐Osuna³

et al. 2015

TCRM

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Dry eye is a common disorder in routine ophthalmological practice, and a better understanding of the complex pathophysiology is leading to improved treatment. Thealoz Duo® is a novel artificial tear preparation containing two active ingredients: Trehalose, a naturally occurring disaccharide with anhydrobiotic functions in many organisms, and hyaluronate, a widely distributed anionic glycosaminoglycan polysaccharide with lubricative and water-retaining properties in biological systems. In a randomized, single center, open label, crossover study, 17 adult patients with moderate-to-severe dry eye syndrome were randomized to treatment with Thealoz Duo® (combining trehalose and hyaluronic acid) or Systane®. Patients received 7 days of treatment. The primary efficacy variable was patient satisfaction evaluated by a 0–100 visual analog scale evaluated on days 0 and 7 of treatment. Secondary parameters included ocular surface disease index (OSDI), symptoms of dry eye, ocular staining scores (fluorescein and lissamine green), ocular clinical signs, Schirmer test, tear breakup time, and global efficacy assessed by the patient and the investigator. Seventeen patients were included. Patient satisfaction improved from 44.5±19.0 to 70.2±19.2 mm during Thealoz Duo® treatment and from 47.2±23 to 57.1±19.1 mm during Systane® treatment (P=0.043, mixed-effects analysis of covariance). Two secondary efficacy parameters (dry eye symptoms and the impact of their symptoms on work) showed statistically significant advantages for Thealoz Duo® over Systane®. There were no statistically significant advantages for Systane® over Thealoz Duo® for any measured parameter. No adverse events were reported. Thealoz Duo® appears to be an effective combination of two active ingredients for the treatment of dry eye and is at least as effective as Systane®.

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“…and water efflux resulting in cell shrinkage, distortion and preferential hydration (Huang and Tunnacliffe 2007). A wide variety of pre-incubation times have been documented for trehalose; for instance, Wikstr€ om et al (2012) reported an upper pre-incubation period of 12 days for the cryopreservation of microencapsulated human retinal pigment epithelial cells, while on the other extreme, the pre-incubation period was limited to 15 min for human corneal epithelium cells in 2D (Hovakimyan et al 2012).…”

Section: Effect Of Cell Culture Condition and Trehalose On Hek Cell Vmentioning

confidence: 99%

Trehalose effectiveness as a cryoprotectant in 2D and 3D cell cultures of human embryonic kidney cells

Hara

Tottori

Anders

et al. 2016

Artificial Cells, Nanomedicine, and Biotechnology

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Post cryopreservation viability of human embryonic kidney (HEK) cells under two-dimensional (2D) and three-dimensional (3D) culture conditions was studied using trehalose as the sole cryoprotective agent. An L (3) Taguchi design was used to optimize the cryoprotection cocktail seeding process prior to slow-freezing with the specific aim of maximizing cell viability measured 7 days post thaw, using the combinatorial cell viability and in-vitro cytotoxicity WST assay. At low (200 mM) and medium (800 mM) levels of trehalose concentration, encapsulation in alginate offered a greater protection to cryopreservation. However, at the highest trehalose concentration (1200 mM) and in the absence of the pre-incubation step, there was no statistical difference at the 95% CI (p = 0.0212) between the viability of the HEK cells under 2D and 3D culture conditions estimated to be 17.9 ± 4.6% and 14.0 ± 3.6%, respectively. A parallel comparison between cryoprotective agents conducted at the optimal levels of the L study, using trehalose, dimethylsulfoxide and glycerol in alginate microcapsules yielded a viability of 36.0 ± 7.4% for trehalose, in average 75% higher than the results associated with the other two cell membrane-permeating compounds. In summary, the effectiveness of trehalose has been demonstrated by the fact that 3D cell cultures can readily be equilibrated with trehalose before cryopreservation, thus mitigating the cytotoxic effects of glycerol and dimethylsulfoxide.

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Evaluation of Protective Effects of Trehalose on Desiccation of Epithelial Cells in Three Dimensional Reconstructed Human Corneal Epithelium

Cited by 31 publications

References 18 publications

Effect of different lubricant eye gels on tear film thickness as measured with ultrahigh‐resolution optical coherence tomography

Effect of different lubricant eye gels on tear film thickness as measured with ultrahigh‐resolution optical coherence tomography

A randomized crossover study comparing trehalose/hyaluronate eyedrops and standard treatment: patient satisfaction in the treatment of dry eye syndrome

Trehalose effectiveness as a cryoprotectant in 2D and 3D cell cultures of human embryonic kidney cells

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