Evaluation of Radical Scavenging and Metal Chelating Potential of Cameroonian Propolis and Isolation of Some Chemical Constituents

Sawalda, Mathieu; Tamfu, Alfred Ngenge; Fadimatou, Fadimatou; Essongue, Cadet François; Talla, Emmanuel; Henoumont, Céline; Laurent, Sophie; Shaheen, Farzana; Mbafor, Joseph Tanyi

doi:10.25135/rfac.11.2206.2487

Cited by 5 publications

(3 citation statements)

References 29 publications

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“…Plants and foods contain various compounds with antioxidant properties and it requires a careful selection of multiple and complementary methods to decipher the antioxidant potential of samples since they involve different mechanisms and sensitivities. [53,54] Phenolic compounds and extracts that contain them have demonstrated remarkable bioactivities such as antioxidant, anticancer, antimicrobial, anti-inflammatory and antidiabetic activities and being capable of reducing neurodegenerative and cardiovascular diseases. [48,55] Both extracts displayed excellent antioxidant activity and in some assays were more active than the standards used.…”

Section: Discussionmentioning

confidence: 99%

HPLC-DAD Phenolic Composition, Antioxidant, Anticholinesterase, Antidiabetic and Anti-quorum Sensing Properties of Bitter Kola (Garcinia kola) and Kolanut (Cola acuminata)

Boudiba¹,

Küçükaydın²,

Tamfu³

et al. 2023

Pharmacogn. Res.

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Background: Eating Cola acuminata and Garcinia cola nuts in African societies symbolizes in socio-cultural hospitality. They stimulate the nervous system, reduce fatigue and sleep. Objectives: To determine the phenolic composition and bioactivities of G. kola and C. acuminata. Materials and Methods: Hydro-ethanol extracts of the nuts were prepared and their phenolic profiles determined using HPLC-DAD. Antioxidant, anticholinesterase, antidiabetic, antimicrobial, antibiofilm and anti-quorum sensing properties were determined. Results: The most abundant phenolic compound was caffeic acid (105.4±0.75 mg/g) in C. acuminata and myricetin (277.2±0.90 mg/g) in G. kola. The extracts showed good antioxidant activity in five complementary assays and G. kola was more active than both α-tocopherol and BHA standards in the DPPH • , CUPRAC and ABTS •+ assays while C. acuminata was more active than only the α-tocopherol standard in the same assays. Activities were close to those of standards in the β-Carotene-linoleic acid and metal chelation assays. Both extracts had good inhibition of Butyrylcholinesterase (BChE) and Acetylcholinesterase (AChE) with IC 50 values 63.27±0.98 µg/mL and 94.15±1.05 µg/mL for C. acuminata and G. kola respectively compared to 5.50±0.25 µg/mL for galantamine in the AChE assay. In the BChE assay, the inhibitory activity was higher for G. kola (IC 50 = 38.66±0.80 µg/ mL) that the standard galantamine (IC 50 = 42.20±0.48 µg/mL) while that for C. acuminata (IC 50 = 87.31±0.77 µg/mL) was moderate. The extracts inhibited α-amylase and α-glucosidase with G. kola (IC 50 =18.43±0.74 µg/mL) being more active than standard acarbose (IC 50 =20.52±0.84 µg/mL in the α-glucosidase assay. The nuts could inhibit expression of virulence factors in Chomobacterium violaceum CV12472 by disrupting violacein production and flagellated Pseudomonas aeruginosa PA01 by disrupting swarming motility. Conclusion: The results indicate good nutraceutical potential of both nuts.

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Section: Discussionmentioning

confidence: 99%

HPLC-DAD Phenolic Composition, Antioxidant, Anticholinesterase, Antidiabetic and Anti-quorum Sensing Properties of Bitter Kola (Garcinia kola) and Kolanut (Cola acuminata)

Boudiba¹,

Küçükaydın²,

Tamfu³

et al. 2023

Pharmacogn. Res.

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“…Lipid peroxidation inhibition measurement activity was performed by the β-carotene-linoleic acid test system with slight modifications [ 82 ]. The DPPH • and ABTS •+ radical scavenging activities were evaluated spectrophotometrically as previously described [ 83 , 84 ]. The cupric-reducing antioxidant capacity (CUPRAC) test was performed using the method reported earlier [ 85 , 86 ].…”

Section: Methodsmentioning

confidence: 99%

Assessment of Chemical Composition and In Vitro Antioxidant, Antidiabetic, Anticholinesterase and Microbial Virulence-Quenching Effects of Salad Burnet (Sanguisorba minor L.) Harvested from Algeria

Haouam,

Boudiba,

Tamfu

et al. 2023

Plants

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Sanguisorba minor is a medicinal vegetable used in seasoning desserts, juices, and beverages. An evaluation of the total flavonoid, phenolic, tannin and anthocyanin contents indicated that these classes of compounds are distributed variably in the different fractions. In summary, the HPLC-DAD analyses enabled the identification and quantification of thirteen phenolic compounds in an ethyl acetate extract (EAE), nine in a dichloromethane extract (DCME), seven in an aqueous extract (AQE) and four in a butanol extract (BE). Rutin was the most abundant phenolic compound in the BE (278.4 ± 1.20 µg/g) and AQE (32.87 ± 0.23 µg/g) fractions, while apigenin was the most abundant in the DCME (84.75 ± 0.60 µg/g) and EAE (156.8 ± 0.95 µg/g) fractions. The presence of phenolic compounds in the fractions conferred good antioxidant capacity, especially the EAE and DCME fractions, which both exhibited higher antioxidant effects than BHA and α-tocopherol in DPPH• and CUPRAC assays. Additionally, in the ABTS•+ assay, EAE (IC50 = 9.27 ± 0.33 µg/mL) was more active than α-tocopherol (IC50 = 35.50 ± 0.55 µg/mL), and BHA (IC50 = 12.70 ± 0.10 µg/mL). At 200 µg/mL, the fractions inhibited acetylcholinesterase and butyrylcholinesterase as well as α-amylase and α-glucosidase, indicating that they can slow neurodegeneration and hyperglycemia. Minimal inhibitory concentration (MIC) values ranged from 0.312 mg/mL to 1.25 mg/mL, and fractions showed good biofilm inhibition against Staphylococcus aureus and Escherichia coli. The extracts exhibited good violacein inhibition in Chromobacterium violaceum CV12472 and Chromobacterium violaceum CV026, despite the supply of external acyl-homoserine lactone to CV026. The antioxidant, quorum-sensing, antibiofilm and enzyme inhibition attributes indicate the potential for the application of S. minor as a food preservative.

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“…Substances which can inhibit the destructive effects of ROS, RNS and free radicals and referred to as antioxidants. Oxidative stress occurs in the situation of an imbalance between ROS and RNS production with respect to antioxidants and the oxidative damage that results from this can spread over most cells and tissues (Talla et al, 2017;Alain et al, 2022;Sawalda et al, 2022). Most antioxidant substances from natural and synthetic sources exist and they are capable of of suppressing ROS and RNS and protect the living systems against oxidative stress (Tamfu et al, 2020d).…”

Section: Introductionmentioning

confidence: 99%

Chemical Composition and Evaluation of Anti-tyrosinase and Anti-Oxidative Effects of Topical Cream Formulation from Acacia sieberiana, Vitellaria paradoxa and Beeswax

et al. 2023

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Skin diseases can get natural therapies from medicinal plant-based products. In this study, a topical cream was formulated from ethanol extract of Acacia sieberiana, beeswax and Vitellaria paradoxa (shea) butter. GC-MS characterization with co-injection of the topical cream revealed Stearic acid (31.43%), Palmitic acid (23.15%), Oleic acid (21.44%) and Linoleic acid (16.20%) as the major components. Seven phenolic conpounds were identified and quantified by HPLC- DAD and Ferulic acid (12.81±0.26 mg/g) was the most abundant. The cream showed good antioxidant properties evaluated through ?-Carotene-linoleic acid assay, DPPH• radical scavenging, ABTS•+ assay, CUPRAC assay, and metal chelating assay. The cream had higher activity in the DPPH• assay (IC50 = 32.10±0.84 µg/mL), ABTS•+ assay (IC50 = 22.49±0.62 µg/mL) and CUPRAC assay (IC50 = 49.27±0.79 µg/mL) than ?-Tocopherol. The antioxidant effects are an indication that the cream can reduce oxidative stress on the skin including aging, carcinogenesis and inflammation. At 100 µg/mL, the topical cream showed tyrosinase inhibition of 48.23±0.87% regarded as relatively good compared to the standard tyrosinase inhibitor kojic acid, which showed 79.50±0.32% inhibition at the same concentration. The cosmetic cream was able to inhibit the melanin production rate-limiting enzyme, tyrosinase, indicating that it can control hyperpigmentation and skin spots.

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Evaluation of Radical Scavenging and Metal Chelating Potential of Cameroonian Propolis and Isolation of Some Chemical Constituents

Cited by 5 publications

References 29 publications

HPLC-DAD Phenolic Composition, Antioxidant, Anticholinesterase, Antidiabetic and Anti-quorum Sensing Properties of Bitter Kola (Garcinia kola) and Kolanut (Cola acuminata)

HPLC-DAD Phenolic Composition, Antioxidant, Anticholinesterase, Antidiabetic and Anti-quorum Sensing Properties of Bitter Kola (Garcinia kola) and Kolanut (Cola acuminata)

Assessment of Chemical Composition and In Vitro Antioxidant, Antidiabetic, Anticholinesterase and Microbial Virulence-Quenching Effects of Salad Burnet (Sanguisorba minor L.) Harvested from Algeria

Chemical Composition and Evaluation of Anti-tyrosinase and Anti-Oxidative Effects of Topical Cream Formulation from Acacia sieberiana, Vitellaria paradoxa and Beeswax

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