Evaluation of recombinant outer membrane protein based vaccine against Salmonella Typhimurium in birds

Prejit,; Agarwal, Rajesh; Porteen, K.; Dubal, Z. B.; Asha, K.; Shweta, Singh; Ripan, Biswas

doi:10.1016/j.biologicals.2013.01.003

Cited by 18 publications

(13 citation statements)

References 34 publications

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“…14,15 Partially purified OMPs have better immunogenicity than both killed and live Salmonella vaccines. 15,16 Salmonella OMPs delivered orally induced mucosal immune response in chickens. 16 Immunogenicity of subunit vaccines could be augmented when delivered with adjuvants.…”

Section: Introductionmentioning

confidence: 99%

Surface engineered polyanhydride-based oral <em>Salmonella </em>subunit nanovaccine for poultry

Sankar¹,

Markazi²,

Dhakal³

et al. 2018

IJN

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PurposeSalmonellosis is a severe economic threat in poultry and a public health concern. Currently available vaccines are ineffective, and thus, developing effective oral Salmonella vaccine is warranted. Especially, a potent oral vaccine such as the mucoadhesive polyanhydride nanoparticle (PNP) protects the vaccine cargo and delivers to intestinal immune sites to elicit robust mucosal immunity and mitigate Salmonella colonization and shedding.Materials and methodsWe designed a Salmonella subunit vaccine using PNP containing immunogenic Salmonella outer membrane proteins (OMPs) and flagellar (F) protein-entrapped and surface F-protein-coated PNPs (OMPs-F-PNPs) using a solvent displacement method. Using high-throughput techniques, we characterized the OMPs-F-PNPs physicochemical properties and analyzed its efficacy in layer birds vaccinated orally.ResultsThe candidate vaccine was resistant in acidic microenvironment and had ideal physicochemical properties for oral delivery in terms of particle size, charge, morphology, biocompatibility, and pH stability. In vitro, in vivo, and ex vivo studies showed that F-protein surface-anchored nanoparticles were better targeted to chicken immune cells in peripheral blood and splenocytes and intestinal Peyer’s patch sites. In layer chickens inoculated orally with OMPs-F-PNPs, substantially higher OMPs-specific IgG response and secretion of Th1 cytokine IFN-γ in the serum, enhanced CD8+/CD4+ cell ratio in spleen, and increased OMPs-specific lymphocyte proliferation were observed. OMPs-F-PNPs vaccination also upregulated the expression of toll-like receptor (TLR)-2 and -4, TGF-β, and IL-4 cytokines’ genes in chicken cecal tonsils (lymphoid tissues). Importantly, OMPs-F-PNPs vaccine cleared Salmonella cecal colonization in 33% of vaccinated birds.ConclusionThis pilot in vivo study demonstrated the targeted delivery of OMPs-F-PNPs to ileum mucosal immune sites of chickens and induced specific immune response to mitigate Salmonella colonization in intestines.

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Section: Introductionmentioning

confidence: 99%

Surface engineered polyanhydride-based oral <em>Salmonella </em>subunit nanovaccine for poultry

Sankar¹,

Markazi²,

Dhakal³

et al. 2018

IJN

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“…The immunogenic potential was earlier studied and it was observed to be imunodominant and thermostable protein (Verma et al, 2009;Jailkhani et al, 2007). Hence, OmpC is considered as a potential candidate for the development of a subunit vaccine against Salmonellosis (Prejit et al, 2013). Further, OmpC-OmpF mutant was found to possess attenuated virulence (Chatfield et al, 1991) revealing its role in pathogenesis and was also observed to confer long lasting and specific humoral response against the pathogen (Secundino et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

“…Porins represent the most abundant class of Omp(s) that have been elucidated to be protective (Calderon et al, 1986;Isibasi et al, 1988) in few serovars of Salmonella (Prejit et al, 2013). They have important role in the maintenance of bacterial physiology aiding transport of small hydrophilic molecules (Nikaido, 1996), having a potential role as immunogens in diagnostic assays and vaccination (Hamid and Jain, 2008;Aron et al, 1993;Secundino et al, 2006;Singh and Sharma, 1999).…”

Section: Introductionmentioning

confidence: 99%

“…They have important role in the maintenance of bacterial physiology aiding transport of small hydrophilic molecules (Nikaido, 1996), having a potential role as immunogens in diagnostic assays and vaccination (Hamid and Jain, 2008;Aron et al, 1993;Secundino et al, 2006;Singh and Sharma, 1999). Specific humoral and cellular immune responses are mounted against Salmonella Omp(s) (Aron et al, 1993;Secundino et al, 2006;Begum et al, 2008;Bhat and Jain, 2010;Prejit et al, 2013). Several outer membrane Salmonella proteins have been characterized, evaluated and found to be immunodominant in poultry (Wyszynska et al, 2004;Begum et al, 2008;Meenakshi et al, 1999;Nicholas and Andrews, 1991).…”

Section: Introductionmentioning

confidence: 99%

“…Outer membrane proteins when administered together were found to control fecal shedding of organisms in vaccinated birds (Meenakshi et al, 1999;Prejit et al, 2013). These porins of Salmonella contain T cell immunodominant antigenic regions and are involved in providing protection in typhoid fever and murine salmonellosis (Quinonez et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

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Heterogeneous expression and functional evaluation of in silico characterized recombinant OmpC of Salmonella Typhimurium as a functional poultry vaccine to eradicate zoonotic transmission

Jha¹,

Kumar²,

Saxena³

et al. 2015

Afr. J. Biotechnol.

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Salmonellosis is one of the major global health concerns leading to millions of deaths annually. The present vaccines not being up to the mark necessitate the need for the development of new generation vaccines. Outer membrane proteins (Omps) of several Gram negative bacteria have been investigated and found to be immunogenic and protective. The present study explores the potential of a major porin protein (OmpC) of Salmonella Typhimurium, as a vaccine candidate. The OmpC 3D structure and its potential to bind effectively with antibodies and generate humoral response was investigated using in silico docking, and expressed in a heterogeneous Escherichia coli M15 host strain. The rOmpC was purified and its immunopotential was evaluated in vitro by western blotting and in vivo in three weeks old chicks. The recombinant OmpC produced a significant humoral response and in vaccinated birds 100% survival rate was observed along with delay in the shedding of organism in droppings. These findings indicate that the rOmpC vaccination prevents mortality in chicken and lowers fecal shedding in droppings.

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Advances in Vaccines for Controlling Foodborne Salmonella spp. in Poultry

Kogut

Santín

2019

Food Safety in Poultry Meat Production

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Evaluation of recombinant outer membrane protein based vaccine against Salmonella Typhimurium in birds

Cited by 18 publications

References 34 publications

Surface engineered polyanhydride-based oral <em>Salmonella </em>subunit nanovaccine for poultry

Surface engineered polyanhydride-based oral <em>Salmonella </em>subunit nanovaccine for poultry

Heterogeneous expression and functional evaluation of in silico characterized recombinant OmpC of Salmonella Typhimurium as a functional poultry vaccine to eradicate zoonotic transmission

Advances in Vaccines for Controlling Foodborne Salmonella spp. in Poultry

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