Evaluation of Red Cell Hemolysis in Packed Red Cells During Processing and Storage

Makroo, Raj Nath; Raina, Vimarsh; Bhatia, Aakanksha; Gupta, Richa; Majid, Abdul; Thakur, Uday Kumar; Rosamma, N L

doi:10.1016/s0976-0016(12)60005-2

Cited by 15 publications

(26 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Corroborating previous veterinary and human studies, hemolysis increased during storage . The cumulative effect of proinflammatory substances, mostly released from leukocytes and platelets, along with the ATP depletion that reduces the integrity of RBCs membranes, may explain this finding .…”

Section: Discussionsupporting

confidence: 56%

In vitro hemolysis of stored units of canine packed red blood cells

Ferreira

Graça²,

Cardoso³

et al. 2018

J Vet Emergen Crit Care

View full text Add to dashboard Cite

Background -Hemolysis is an important quality parameter of packed red blood cells (pRBCs) that is used to assess the cellular integrity of stored blood units. According to human standards, hemolysis at the end of storage must not exceed 1%, as otherwise it may be responsible for decreased transfusion effectiveness and acute life-threatening reactions.Objectives -This prospective study was designed to evaluate the hemolysis of canine pRBCs stored in an additive solution containing adenine, dextrose, mannitol, and sodium chloride, and to assess its associations with storage time, duration of the collection process, collection disturbances, and with the final volume and PCV of the pRBCs units. Methods -One hundred eighty pRBCs units were collected from canine donors. Hemolysis of the pRBCs units was determined immediately after processing (t = 0). The units were then stored and retested (t = 1) either before administration (during weeks 2, 3, 4, 5, or 6 of storage) or at the end of the storage period (42 d) if not used. Results -Mean hemolysis at t = 0 was 0.09% (SD 0.06) and increased during storage, at a more pronounced rate from the 5th (mean values of 0.52%, SD 0.29) to the 6th week (1.2%, SD 0.72). Almost 51% of the units with 36-42 days of shelf-life showed more than 1% hemolysis. Disturbances in the collection process, the volume of the whole blood units, and the volume of stored pRBCs units or their PCV were not related to pRBCs hemolysis. Conclusions -According to human blood bank recommendations regarding acceptable hemolysis, canine pRBCs stored for more than 35 days should be tested to ensure <1% hemolysis prior to administration. (J Vet Emerg Crit Care 2018; 28(6): 512-517)

show abstract

Section: Discussionsupporting

confidence: 56%

In vitro hemolysis of stored units of canine packed red blood cells

Ferreira

Graça²,

Cardoso³

et al. 2018

J Vet Emergen Crit Care

View full text Add to dashboard Cite

show abstract

“…Proteases released by leukocytes during storage have been reported to cause RBC lysis during storage and are detrimental to their metabolism and viability [23,25]. Storage of red cells causes a progressive increase in hemolysis [26].…”

Section: Discussionmentioning

confidence: 99%

Study of Incubation Conditions for Erythrocytes Osmotic Fragility Testing in Dromedary Camel (Camelus dromedarius)

Islah¹,

Bargaa²,

Youssef³

et al. 2016

International Journal of Research in Environmental Science

View full text Add to dashboard Cite

show abstract

“…46 On the other hand, the US Food and Drug Administration recommends a threshold level for pRBC of <1% after 42 days of storage. 5,19,[47][48][49] In a recent study evaluating the effects of different storage solutions on canine pRBC, the mean percentage of hemolysis at time 31 ranged between 0.91% and 1.13%. 12 In our study, the mean value of the percentage of hemolysis at day 28 was 0.65%, much below the recommended level in human medicine, whereas at day 35 it was 0.84%, just slightly above it.…”

Section: 3-dpg Is a Glycolytic Intermediate That Can Be Found Withimentioning

confidence: 99%

In vitro assessment of quality of citrate‐phosphate‐dextrose‐adenine‐1 preserved feline blood collected by a commercial closed system

Crestani

Stefani

Carminato

et al. 2018

Veterinary Internal Medicne

View full text Add to dashboard Cite

BackgroundOptimal procedure for storage of feline blood is needed. Open‐collection systems have been employed in feline medicine, thus limiting the possibility for storage.ObjectivesTo evaluate indicators of quality of feline blood stored for 35 days at +4°C in a closed‐collection system specifically designed for cats.AnimalsEight healthy adult European domestic shorthair cats with a weight of 5‐6.8 kg.MethodsThis is a case series study. A bacteriological test, CBC, blood smear, pH, osmotic fragility, 2,3‐diphosphoglycerate (2,3‐DPG), and adenosine triphosphate (ATP) measurement were performed weekly on whole blood (WB) units from day 1 to day 35 after donation. The hemolysis index, lactate and potassium concentrations, prothrombin time (PT), activated partial thromboplastin time (aPTT), and fibrinogen were measured on plasma aliquots.ResultsOne out of eight blood units (BUs) had bacterial growth (Serratia marcescens) at day 35. No significant differences were found regarding CBC, morphology, pH, and osmotic fragility. Despite high inter‐individual variability and low starting levels, significant decreases in the mean concentrations of 2,3‐DPG (T0 1.99 mmol/g Hb, SD 0.52, T35 1.25 mmol/g Hb, SD 1.43; P = .003) and ATP (T0 1.45 mmol/g Hb, SD 0.71, T35 0.62 mmol/g Hb, SD 0.51; P < .001) were detected during the study, as opposed to an increase in hemolysis (T0 0.11 mmol/L, SD 0.07, T35 0.84 mmol/L, SD 0.19; P < .001), lactate (T0 3.30 mmol/L, SD 0.86, T35 13.36 mmol/L, SD 2.90; P < .001), and potassium (T0 3.10 mmol/L, SD 0.21, T35 4.12 mmol/L, SD 0.35; P < .001) concentrations.Conclusions and Clinical ImportanceThe commercial BU kit is appropriate for blood collection and conservation of WB in cats. The maintenance of WB quality indicators during storage is essential for future improvements of feline transfusion medicine.

show abstract

Evaluation of Red Cell Hemolysis in Packed Red Cells During Processing and Storage

Cited by 15 publications

References 12 publications

In vitro hemolysis of stored units of canine packed red blood cells

In vitro hemolysis of stored units of canine packed red blood cells

Study of Incubation Conditions for Erythrocytes Osmotic Fragility Testing in Dromedary Camel (Camelus dromedarius)

In vitro assessment of quality of citrate‐phosphate‐dextrose‐adenine‐1 preserved feline blood collected by a commercial closed system

Contact Info

Product

Resources

About