Evaluation of Reliability of FISH Versus Brightfield Dual-probe In Situ Hybridization (BDISH) for Frontline Assessment of HER2 Status in Breast Cancer Samples in a Community Setting

Abstract

SISH is the most reliable of the BDISH methods, with sensitivity and specificity highly comparable with FISH. It is also less deleterious than other BDISH methods, producing signals that were more distinct and therefore more readily analyzable even in poorly preserved tissue.

High agreement between whole slide imaging and optical microscopy for assessment of HER2 expression in breast cancer

“…We used the DDISH method that has been FDA approved as an in situ hybridization method, which is fully automated, has high concordance with FISH, and has the advantages of a bright field test [17,34].…”

Problems In Determining Her2 Status In Breast Carcinoma

“…FISH is the least affected method by pre-analytical processes and results in the least damage to the tissue. FISH to determine HER2 status is considered the gold standard (12). Disadvantages of FISH analysis are the long technical procedures, signals fading over time, and failure to store slides for a long-term, requirement of fluorescence microscopy and expertise for evaluation.…”

Determination of HER2 Gene Amplification in Breast Cancer using Dual-color Silver Enhanced in situ Hybridization (dc-SISH) and Comparison with Fluorescence ISH (FISH)

“…Spot-like fluorescent signals are evaluated under highly sensitive fluorescent microscopes in the FISH method; while shining opaque silver is easily seen under the light microscope in the SISH method. Moreover, the results can be evaluated quickly and histopathological evaluation of the same tissue section can be performed at the same time (Francis et al, 2009;Papouchado et al, 2010;Ying et al, 2010;Lee et al, 2012;Schiavon et al, 2012).…”

“…Pre-analytic factors affect the test results less than immunohistochemistry. Nevertheless, the FISH technique is more expensive, it necessitates special equipment and educated personnel, its application process lasts longer, and a fluorescent microscope is required for the evaluation of the test (Francis et al, 2009;Papouchado et al, 2010;Ying et al, 2010;Arnould et al, 2012;Schiavon et al, 2012). The SISH method, which can be an alternative to the FISH technique, is comprised of a multimer technology, combined with enzyme metallography, which allows for the identification of the HER2 oncogene amplification with silver marking.…”