Evaluation of response of dynamics change in bioaugmentation process in diesel-polluted seawater via high-throughput sequencing: Degradation characteristic, community structure, functional genes

Xue, Junmin; Shi, Ke; Chen, Chuan; Bai, Yu; Cui, Qinqin; Li, Nana; Fu, Xinge; Qiao, Yanlu

doi:10.1016/j.jhazmat.2020.123569

Cited by 39 publications

(10 citation statements)

References 32 publications

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“…Moreover, examining the functions of bacterial communities is critical for estimating and understanding of how bacterial communities respond to the changes of ecosystem (Ward et al, 2017;Escales et al, 2019). Recent investigations on functional potential prediction of bacteria by employing PICRUSt2 in different environments, including soils (Toole et al, 2021), seawater (Xue et al, 2021), marine sediment (Su et al, 2018) are in help to decipher the detailed function variations successfully. In addition, benefiting from the function prediction of bacteria from the 16S rRNA gene sequences solely, diverse unknown microbes could be identified phylogenetically (Tsaban et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Switch of Bacteria Community Under Oxygen Depletion in Sediment of Bohai Sea

Guo

Song

et al. 2022

Front. Mar. Sci.

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Regular oxygen depletion is occurring every summer in the depression area of the Bohai Sea. The community structure and potential functions of microbes in expanding marine hypoxic area are of great importance due to their roles in biogeochemical cycling. In this study, the diversity and distribution of bacteria based on 16S rRNA gene in sediment along an inshore-offshore transect across the oxygen-depletion area in the Bohai Sea was investigated in June, July and August of 2018 by employing high-throughput sequencing. Results revealed that the bacteria community was dominated by Proteobacteria (42.67%), Actinobacteria (14.13%), Chloroflexi (13.02%), Acidobacteria (8.01%), and Bacteroidetes (6.30%). During oxygen depletion, the bacteria community from inshore site A3 subjected to dramatic variation from June to August, but the composition tended to be stable in sites from the depression area along the transect. Distinct switch of bacteria from aerobic to anaerobic group was observed when the DO concentration <4.2 mg/L, typically represented by dominance of Anaerolineaceae in August sample. Further, KEGG prediction by PICRUSt confirmed the variations by showing significant difference in functional pathways, especially the nitrogen metabolisms, before and after DO depletion (p < 0.05). These variations could be influenced by depth, NO2– concentration and DO availability based on RDA analysis. The details in diversity and composition of bacteria under continually observation provide insights into both instant and long-term response of bacteria community to oxygen depletion, and the distinct functional switch under this process expands our knowledge on the metabolic character of bacteria in worldwide hypoxia areas.

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Section: Introductionmentioning

confidence: 99%

Switch of Bacteria Community Under Oxygen Depletion in Sediment of Bohai Sea

Guo

Song

et al. 2022

Front. Mar. Sci.

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“…In recent years, with the rapid development of high throughput sequencing technology, plant rhizosphere microflora research has achieved great progress [10] [11], mainly reflected in plant rhizosphere microbial screening, the identification of genes that heavy metal tolerance, tolerance and the succession of microbial community characteristics and driving factors analysis, and artificial wetland biological strengthening technology [12] [13]. The study found that plant growth-promoting bacteria (PGPB) under Cr stress can promote plant growth through mineral phosphate solubilization, nitrogen fixation, indole-3-acetic acid, siderophores, hydrogen cyanide, and ammonia production [14].…”

Section: Introductionmentioning

confidence: 99%

Effects pf Cr Stress on Bacterial Community Structure and Composition in Rhizosphere Soils of <i>Iris Tectorum</i>

Wang¹,

Gu²,

Yang³

et al. 2022

Preprint

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With the rapid development of industry, Cr has become one of the major heavy metal pollutants in soil, which has caused serious effects on the soil's ecological environment. However, the effects of Cr stress on bacterial communities in plant rhizosphere soils remain unclear. In this study, I. tectorum was selected as the research object, and 16S rRNA high-throughput sequencing technology was used to analyze the effects of Cr stress on the structure and diversity of the bacterial community in the rhizosphere soil of I. tectorum. The results showed that I. tectorum had strong tolerance and enrichment to Cr. However, under Cr stress, the diversity and abundance index of rhizosphere bacteria decreased by 8.5% and 6.8% on average, and the Sobs index decreased by 7.6%. Moreover, the bacterial community changed by 20.1% due to the addition of Cr, further leading to a 15.9% decrease in the common species of the bacterial community, among which Proteobacteria, Actinobacteria, Chloroflexi and Acidobacteriota accounted for more than 74.8% of the total sequence. According to the symbiosis network diagram, it was found that under a two-cultivated pattern, the synergizing effect between dominant bacteria was significantly enhanced, and the soil microenvironment was improved. Redundancy analysis showed that C, N, and P nutrient elements and Cr contents in uncontaminated and contaminated soils were the primary driving factors for the succession of I. tectorum rhizosphere bacterial community, and the response was stronger after Cr(Ⅵ) was added. In conclusion, the results of this study will provide insights into the response of rhizosphere bacterial communities to heavy metal Cr and the interactions between wetland plants and rhizosphere bacteria in wetland phytoremediation.Keywords: Cr stress; rhizosphere bacterial community; Iris tectorum; 16S rRNA sequencing technology; Phytoremediation

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“…Microbial communities responsible for the biogeochemical cycles are being truncated since diesel oil is toxic to general organisms (Kayode-Isola et al 2008). The quantity and quality of oil mixtures and the properties of the affected ecosystems determine the durability of oil pollution (Xue et al 2021). Conventional marine oil pollution remediation techniques include physical, chemical and thermal treatments which are Edited by Xiu-Qiu Peng costly and often cause secondary pollution.…”

Section: Introductionmentioning

confidence: 99%

Isolation and characterization of a high-efficiency marine diesel oil-degrading bacterium

Jin

Mei

et al. 2021

Pet. Sci.

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In the recent 50 years, marine oil spills had resulted in severe environmental pollution problems worldwide. In this study, 12 petroleum-degrading strains named MJ1 to MJ12, which can use diesel oil as the sole carbon source for growth, were isolated from the seawater in Jiaozhou Bay, China. Strain MJ4 has the highest diesel-degrading rate which is up to 26.54% in 5 days with the diesel oil concentration of 10 g/L. According to the BLAST research, 16SrRNA sequence of MJ4 showed 99% similarity to Bacillus megaterium strain. Single-factor experiments and response surface methodology were carried out to optimize the environmental factors and their reciprocal action for affecting the diesel oil degradation process of Bacillus sp. MJ4. Results of single-factor experiments revealed that the highest degradation rate was obtained with temperature of 28 °C, pH of 8.8, diesel oil concentration of 25 g/L, P/N ratio of 0.56, nitrogen and phosphorus dosage quantity of 0.35 g/L and 0.18 g/L, respectively. A nonlinear regression equation of diesel oil degradation rate and pH, temperature, P/N ratio was obtained. The model predicted the maximum degradation rate of 72.21% with temperature of 28 °C, pH of 8.88, P/N ratio of 0.31, respectively.

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Evaluation of response of dynamics change in bioaugmentation process in diesel-polluted seawater via high-throughput sequencing: Degradation characteristic, community structure, functional genes

Cited by 39 publications

References 32 publications

Switch of Bacteria Community Under Oxygen Depletion in Sediment of Bohai Sea

Switch of Bacteria Community Under Oxygen Depletion in Sediment of Bohai Sea

Effects pf Cr Stress on Bacterial Community Structure and Composition in Rhizosphere Soils of <i>Iris Tectorum</i>

Isolation and characterization of a high-efficiency marine diesel oil-degrading bacterium

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