Evaluation of salivary and serum lipid peroxidation, and glutathione in oral leukoplakia and oral squamous cell carcinoma

Abstract

Lipid peroxidation induced by reactiveoxygen species (ROS) is involved in the pathogenesis of malignancy. Overall, lipid peroxidation levels are indicated by malondialdehyde (MDA), which is the most frequently used biomarker to detect oxidative changes. Antioxidant defense systems such as glutathione (GSH) limit cell injury induced by ROS. Therefore, MDA and GSH can be used to monitor oxidative stress (OS). Hence, this study aimed to evaluate and compare both salivary and serum levels of MDA and GSH in oral leukoplakia and oral squamous cell carcinoma (OSCC) patients, and healthy controls. The study included 100 subjects comprising 30 apparently healthy controls, 30 patients with oral leukoplakia and 40 clinically and histologically diagnosed patients with OSCC. Saliva and blood samples were obtained and evaluated for MDA and GSH. The study revealed enhanced MDA levels in saliva and serum in oral leukoplakia and OSCC patients as compared to controls. On the other hand, significant decreases were seen in serum and salivary GSH levels in oral leukoplakia and OSCC patients as compared to controls. Augmentation of OS in blood and saliva is reflected by increase in MDA and decrease in GSH levels, indicating that tumor processes cause an imbalance of oxidant-antioxidant status in cell structures.(J Oral Sci 56, 135-142, 2014)

Oxidative Stress and Oral Mucosal Diseases: An Overview

“…Metgud et al showed a significant increase of MDA and reduction of GSH progressively from healthy controls to pre-cancerous and to patients with OSCC, in line with a state of oxidative stress related to OSCC. This finding supports the hypothesis that ROS metabolism is markedly altered in cancer cells, leading to enhanced production of ROS compared to non-neoplastic cells, and the suppression of the antioxidant system that mediate body's defense mechanisms (90). For OSCC, as for most other cancer types, the prognosis depends largely on lifestyle factors, medical comorbidity, grading and tumor staging.…”

“…Another interesting study, carried out by Metgud et al, reported the diagnostic efficacy of saliva in evaluating levels of MDA and GSH in smoking patients with OSCC (90). Tobacco represents an exogenous source of ROS that subsequently leads to oxidative stress.…”

Validated analytical method to determine new salivary lipid peroxidation compounds as potential neurodegenerative biomarkers

“…Some analytical methods have been developed to study salivary oxidative stress biomarkers (glutathione peroxidase, catalase, protein carbonyls, 8-hydroxy-2'-deoxyguanosine), but few of them determined lipid peroxidation compounds, such as malondialdehyde (MDA) [14][15][16], and prostanoids (e.g. 15-F 2t -IsoP) [10].…”

Effect of iron nanoparticles on free radical oxidation process in blood of rats with Pliss lymphosarcoma

“…Принимая во внимание вышеизложенное и несомненную патогенетическую роль свободнорадикальных процессов в канцерогенезе и прогрессии опухолей [10] В работе использовали ультрадисперсный порошок железа, полученный из крупнодисперсных порошков с помощью плазменной технологии, основанной на испарении сырья (крупнодисперсного порошка или прутка) в плазменном потоке с температурой 5000-6000 К и конденсации пара до ультрадисперсных частиц требуемого размера (дисперсность частиц 30-50 нм). Форма НЧ была близка к сферической.…”