Evaluation of Salivary and Serum Antioxidant and Oxidative Stress Statuses in Patients with Chronic Periodontitis: A Case-Control Study

Abstract

Aim: Local bacteria stimulate polymorphonuclear neutrophils to release reactive oxygen species in periodontitis. Increased levels of oxidative stress play a significant role in the pathogenesis of periodontitis. Therefore, this study aimed to evaluate total salivary and serum antioxidant capacity and malondialdehyde in patients with chronic periodontitis.Materials and methods: Fifty-five healthy subjects and 55 patients with chronic periodontitis, with an age range of 30–50 years, were evaluated. After clinical examination and case selection, unstimulated whole saliva was collected in the morning. Blood samples were taken from the antecubital vein. Total antioxidant capacity and malondialdehyde levels were evaluated by spectrophotometric assay. Data were analyzed with t-test, using Stata.11 software program.Results: The periodontitis group exhibited lower salivary (0.16) and serum (0.36) total antioxidant capacity (P = 0.11) compared to the control group. Mean salivary malondialdehyde levels in the case and control groups were 0.80 ± 0.09 and 0.42 ± 0.08, respectively. The results showed significantly higher levels of salivary and serum malondialdehyde in the periodontitis group. Gender did not have any effect on antioxidant and oxidative stress levels.Conclusion: This study indicated increased levels of salivary and serum oxidative stresses in patients with chronic periodontitis. Total antioxidant capacity was mildly lower in the saliva and serum of these patients. Higher malondialdehyde levels with no changes in antioxidant status can result in systemic and local complications in these patients.

Superoxide Dismutase, Uric Acid, Total Antioxidant Status, and Lipid Peroxidation Assay in Chronic and Aggressive Periodontitis Patients

“…Significant changes in MDA levels with little or no change in antioxidant status were found in saliva and serum of patients with CP, indicating increased oxidative stress. 24,25,29,30 The review on studies regarding the activities of enzymatic antioxidants revealed that the results of the studies are contradicting to each other. Some studies showed that increase in SOD is associated with periodontitis, whereas others showed increased activities of enzymatic antioxidants as a protective reaction found among the people with periodontitis.…”

Comparative Estimation of Salivary Total Antioxidant Capacity in Periodontal Health and Chronic Periodontitis - A Pilot Study

“…Baltacıoğlu et al [14] found that both lipid peroxidation levels and TAOC in saliva and serum is reduced in patients with CP while Ahmadi-Motamayel et al [15] reported slightly lower TAOC in the periodontitis group compared to healthy control but was not statistically significant.…”

Effect of glutamine ingestion on the progression of induced periodontitis: experimental study in rats

“…This process is able to stimulate the inflammatory response that causes the destruction of the periodontal tissues 11,12 . In addition to the antimicrobial effect 13 , another relevant point is the effect of stimulating the immune system through glutamine.…”