Evaluation of selected Indian traditional folk medicinal plants against Mycobacterium tuberculosis with antioxidant and cytotoxicity study

Tawde, K.V.; Gacche, Rajesh N.; Pund, Mahesh

doi:10.1016/s2222-1808(12)60244-8

Cited by 16 publications

(7 citation statements)

References 15 publications

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“…10 The leaf extracts of the plant were found to possess moderate activity against M. tuberculosis but the active principles were not isolated and identified. 22 However, there are very few reports on the anti-mycobacterial activities of the fruit extract of this plant. Recently, hexane extracts of the leaves were found to possess anti-TB activity at an MIC of 50 μg/mL.…”

Section: Discussionmentioning

confidence: 99%

Isolation and Characterization of Antimycobacterial Compounds from Fruits of Aegle marmelos (L.) Correa

Suja

Jose

Lakshmanan

et al. 2018

JCD

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Root extracts of Aegle marmelos, an evergreen tree indigenous to India, is an important ingredient in Ayurvedic preparations used for the treatment of pulmonary tuberculosis. Leaves of the tree are used as a remedy for respiratory ailments in folk medicine. The fruit is extensively used in the treatment of various bacterial diseases such as dysentery, diarrhea, etc. The present study was designed to screen organic solvent extracts of fruits of A. Marmelos for their inhibitory activity on Mycobacterium tuberculosis (M. tuberculosis), and to isolate and identify the compounds responsible for antimycobacterial activity. The minimum inhibitory concentration (MIC) of the extract and purified molecules on virulent laboratory strain M. tuberculosis H37Rv was determined by resazurin microtiter assay (REMA). Structural elucidation of the compounds was carried out employing NMR. Cytotoxicity of the compounds was evaluated against human monocyte THP1-derived macrophages by MTT assay. The hexane extract of A. marmelos fruits inhibited the growth of M. tuberculosis at an MIC of 50 µg/mL. Fractionation of the hexane extract by column chromatography led to the isolation of imperatorin, β-sitosterol, plumbagin, marmesin, marmin, and stigmasterol which individually inhibited the growth of M. tuberculosis. In vitro cytotoxicity assay revealed that the compounds are not cytotoxic at the concentrations that inhibited the growth of M. tuberculosis.

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Section: Discussionmentioning

confidence: 99%

Isolation and Characterization of Antimycobacterial Compounds from Fruits of Aegle marmelos (L.) Correa

Suja

Jose

Lakshmanan

et al. 2018

JCD

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“…Several previous studies showed that the terpenoids present in extract of A. paniculata leaves have enough potential to kill drug resistant Gram positive bacteria, including MTB [11,12]. The anti-inflammatory properties of Andrographis paniculata might be resulted from the inhibition of pro-inflammatory mediators, at least in part via suppression of a signaling pathway such as NF-κB [24].…”

Section: Discussionmentioning

confidence: 99%

“…F.) Nees (AP) (Family: Acanthaceae) have been used in traditional systems of medicine for the treatment of several diseases [10]. Previous studies note that ethanol extract of AP leaf showed promising activity against M. tuberculosis [11]. The methanol extract of AP leaves was exhibited strong in vitro antibacterial activity against Gram positive bacteria including clinical isolates of M.…”

Section: Introductionmentioning

confidence: 99%

Andrographis Paniculata (Burm. F.) Nees Induces Clinical and Sputum Conversion in Pulmonary Tuberculosis Patients

Widhawati¹,

Hanani²,

Zaini³

2015

Med-Science

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“…The antimycobacterial efficacy of ethanolic extract of leaves of Andrographis paniculata has been reported. 17 Andrographolide, an active constituent of the plant, is known to be active against pulmonary tuberculosis, tuberculous meningitis, and pyelonephritis. 7 A randomized double blind placebo controlled clinical evaluation of extract of Andrographis paniculata (KalmCold) reported its efficacy in patients with uncomplicated upper respiratory tract infection.…”

Section: Discussionmentioning

confidence: 99%

Antimycobacterial Efficacy of Andrographis paniculata Leaf Extracts Under Intracellular and Hypoxic Conditions

Bhatter

Gupta

Daswani

et al. 2014

J Evid Based Complementary Altern Med

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The inhibition of the growth of Mycobacterium tuberculosis by the extracts of Andrographis paniculata has been studied using intracellular and axenic hypoxic conditions. The inhibition (confirmed using the gold standard colony forming unit assay) was found to increase with "double stimuli" or higher concentration of the extract. Organic solvent extracts were found to inhibit bacterial growth more than the aqueous extracts under microaerophilic conditions mimicked through axenic and intracellular assays. This could be further explored to evaluate the potential of the plant to be used against nonreplicating/dormant bacilli.

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Evaluation of selected Indian traditional folk medicinal plants against Mycobacterium tuberculosis with antioxidant and cytotoxicity study

Cited by 16 publications

References 15 publications

Isolation and Characterization of Antimycobacterial Compounds from Fruits of Aegle marmelos (L.) Correa

Isolation and Characterization of Antimycobacterial Compounds from Fruits of Aegle marmelos (L.) Correa

Andrographis Paniculata (Burm. F.) Nees Induces Clinical and Sputum Conversion in Pulmonary Tuberculosis Patients

Antimycobacterial Efficacy of Andrographis paniculata Leaf Extracts Under Intracellular and Hypoxic Conditions

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