Evaluation of selective enrichment broths and chromogenic media for Salmonella detection in highly contaminated chicken carcasses

Hyeon, Ji-Yeon; Park, J.-H.; Chon, Jung‐Whan; Wee, S.-H.; Moon, Jin-San; Kim, Y.-J.; Seo, Kun‐Ho

doi:10.3382/ps.2011-01936

Cited by 11 publications

(9 citation statements)

References 29 publications

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“…Several studies have also found out that the low specificity of XLD medium created a high number of false positive identification of Salmonella species (Rall et al, 2005;Hyeon et al, 2012;Yhiler et al, 2015). Rall et al (2005) stated that XLD allows the growth of selective bacteria with Salmonella, Proteus, and Citrobacter that displayed similar characteristics as negative lactose fermentation and negative hydrogen sulfide (H 2 S) production on this classical medium.…”

Section: Discussionmentioning

confidence: 99%

Occurrence and antibiotic resistance of Salmonella spp. in raw beef from wet market and hypermarket in Malaysia

et al. 2018

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Salmonellae are highly pathogenic foodborne bacteria able to cause infection even at low doses. Infection by Salmonella from contaminated foods leads to gastrointestinal disease known as salmonellosis. Raw beef can be a source of human infection if the meat products are not properly handled, stored or cooked. This study aimed to investigate the prevalence and concentration of Salmonella in the raw beef sold at wet markets and hypermarkets in Serdang, Selangor, Malaysia, using MPN-PCR and MPN-plating on Xylose Lysine Deoxycholate (XLD) medium. In addition, Salmonella isolates recovered from the samples were tested for antibiotics susceptibility using Kirby-Bauer antibiotic susceptibility testing. The incidence of Salmonella in the raw beef samples using plating and PCR methods were 64.63% (53/82) and 17.07% (14/82) respectively. The microbial concentration of Salmonella in raw beef samples ranged between 3-4600 MPN/g by MPNplating and 3-30 MPN/g by MPN-PCR approach. All isolates were found to be susceptible to imipenem, gentamicin, kanamycin, and chloramphenicol but resistant to cephalothin. It can be deduced from the results that raw beef can be a reservoir for Salmonella infection and the use of cephalothin (30 μg) in the treatment of infection due to these strains could be ineffective. Preventive measures such as proper temperature control as well as proper handling of raw beef in the market place are crucial to the minimization of any potential health hazard posed by this foodborne pathogen.

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Section: Discussionmentioning

confidence: 99%

Occurrence and antibiotic resistance of Salmonella spp. in raw beef from wet market and hypermarket in Malaysia

et al. 2018

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“…All experimental procedures for the isolation of Salmonella were performed according to our previous study (Hyeon et al, 2012) with minor modification. Briefly, whole chicken carcasses were rinsed with 400 mL of buffered peptone water (BPW; Oxoid, Hampshire, UK) with gentle shaking for 1 min; 25 mL of the rinsate was combined with the same volume of BPW and incubated at 37°C for 24 h. For selective enrichment, 0.1 mL of enrichment was transferred to 10 mL of RappaportVassiliadis (RV) broth (bioMérieux, Marcy l'Etoile, France) and incubated at 42°C for 24 h. A loopful of RV broth culture was seeded onto xylose lysine deoxycholate agar (BD Difco, Detroit, MI) and incubated at 37°C for 24 h. A maximum of three presumptive colonies were subcultured onto nutrient agar (BD).…”

Section: Isolation Of Salmonella From Chicken Carcassesmentioning

confidence: 99%

High Occurrence of Extended-Spectrum β-Lactamase-ProducingSalmonellain Broiler Carcasses from Poultry Slaughterhouses in South Korea

Chon

Jung

Kuk

et al. 2015

Foodborne Pathogens and Disease

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The emergence of antibiotic-resistant foodborne Salmonella has become a major public health problem. Consumption of undercooked poultry contaminated with Salmonella can induce food poisoning in humans. In this study, we investigated the occurrence and antibiotic resistance patterns of Salmonella spp. isolated from 120 chicken carcasses produced in 6 poultry slaughterhouses in South Korea. A total of 11 samples (9.2%) were found contaminated with Salmonella: 5 isolates were serotyped as Salmonella Bellevue strain (slaughterhouse C) and 6 isolates were serotyped as Salmonella Enteritidis strain (slaughterhouse E). Salmonella Bellevue isolates were resistant to five antibiotics (ampicillin, chloramphenicol, nalidixic acid, tetracycline, and trimethoprim/sulfamethoxazole), while Salmonella Enteritidis isolates were resistant to nine antibiotics (ampicillin, cefotaxime, ceftazidime, cefazolin, cephalothin, amikacin, nalidixic acid, streptomycin, and tetracycline). All cephalosporin-resistant Salmonella Enteritidis isolates exhibited the extended-spectrum β-lactamase (ESBL) phenotype and carried the gene encoding CTX-M-15, the most prevalent ESBL enzyme worldwide. Based on molecular subtyping performed using the automated rep-polymerase chain reaction (PCR) system (DiversiLab), the isolates showing ≥ 95 similarity in their rep-PCR banding patterns were classified into 5 pulsotypes. Given that cephalosporins are the drugs of choice for invasive Salmonella infections, the high incidence of ESBL-producing strains in chicken should emphasize the necessity of regular monitoring of the occurrence of antibiotic-resistant ESBL-positive Salmonella strains in poultry meat.

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“…In recent years, the ISO 6579:2002 standard method for detection of Salmonella from primary animal production has been evaluated and widely adopted by several standard laboratories worldwide [8]. This standard method requires four relevant stages which involves 1) non-selective pre-enrichment in modified Buffered Peptone water (BPW); 2) the selective enrichment in Modified Semisolid Rappaport-Vassiliadis (MSRV) or Muller-Kauffmann tetrathionate-novobiocin broth (MKTTn); 3) the selective plating on xylose-lysine-deoxycholate (XLD) agar and an additional suitable selective plate of choice and; 4) confirmation characteristics [5] [9]- [12].…”

Section: Introductionmentioning

confidence: 99%

“…Previously, the Rappaport-Vassiliadis (RV) enrichment broth was modified to the Rappaport-Vassiliadis Soy (RVS) broth which has currently been further modified into the Modified Semisolid Rappaport-Vassiliadis (MSRV). These amendments were aimed at heightening the reliability of the enrichment medium [12] [13]. The MKTT enrichment broth was the first to develop by Muller andwas later supplemented with Ox bile and Brilliant Green in order to enhance selectivity by Kauffmann and Jeffries further included the addition of novobiocin at 40 mg/L in order to inhibit indigenous flora, such as Proteus spp., and henceimproving on the rate of recovery of Salmonella [14]- [16].…”

Section: Introductionmentioning

confidence: 99%

“…There are a good number of selective plating media that have been developed on the basis of the biochemical characteristics of Salmonella, such as α-galactosidase activity in the absence of β-galactosidase activity, C8-esterase activity, catabolism of glucuronate, glycerol, and propylene glycol, andhydrolysis of X-5-gal [19] [20]. In spite of the numerous studies that have aimed at determining the optimal selective media showing high level of sensitivity and specificity for the detection of Salmonella in food and environmental samples [12], this current study was aimed at evaluating the sensitivity and specificity of Brilliant Green (BG) selective plating medium compared with the preferential selective plating medium, Xylose lysine deoxicholate (XLD). This study determined the rate of recovery of Salmonella from the primary production of poultry and compared the sensitivity, specificity, and the rates of false positives and false negatives of the selective enrichment media (MSRV and MKTTn) and the selective plating media (XLD and BG) for the detection of Salmonella according to ISO 6579:2002.…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of the Performance of Two Selective Enrichment Media and Two Selective Plating Media for the Detection of <i>Salmonella</i> from Primary Poultry Production, According to ISO 6579:2002

Yhiler¹,

Bassey²,

Useh³

2015

OJMM

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Salmonellosis remains a major problematic food born zoonosis worldwide. Poultry and poultry products have been frequently incriminated as the major sources for the aetiological agent, Salmonella species. The implementation of appropriate surveillance and monitoring programme for food safety ultimately requires the examination of food for the presence of food borne pathogens. This study was aimed at evaluating the effectiveness of Modified Semisolid Rappaport-Vassiliadis (MSRV) and Muller-Kauffmann tetrathionate-novobiocin (MKTTn) selective enrichment media for the isolation of Salmonella, as well as assessment of the performance of xylose-lysine-deoxycholate (XLD) agar as the classical and preferential selective plating medium by comparing it with Brilliant Green (BG) agar for the detection of Salmonella in the primary production of poultry based on the ISO 6579:2002. Three hundred and seventy-four (374) samples from the primary poultry production system underwent the traditional culture method for the detection of Salmonella involving pre-enrichment, selective enrichment, selective plating and confirmation. The rate of recovery of Salmonella from the entire samples was 59.1%. The rate of recovery of Salmonella from XLD and BG selective plating media when MSRV selective enrichment media was used was 98.2% and 95.5% respectively whereas 90.0% and 64.3% respectively when MKTTn selective enrichment media was used. The highest sensitivity was observed in the combination of MSRV/XLD (0.98), followed by MSRV/BG (0.95). The combination of MSRV/BG had the highest specificity (0.88) followed by MKTTn/BG (0.65). Based on the results of this study, selective enrichment media play a substantial role on the sensitivity and specificity of the selective plating media. MSRV * Corresponding author. N. Y. Yhiler et al. 129 was revealed as the most important selective enrichment medium, necessary for the investigation of Salmonella and that its combination with BG selective plating media yielded the best result for the selective isolation of Salmonella in the primary production of poultry.

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Evaluation of selective enrichment broths and chromogenic media for Salmonella detection in highly contaminated chicken carcasses

Cited by 11 publications

References 29 publications

Occurrence and antibiotic resistance of Salmonella spp. in raw beef from wet market and hypermarket in Malaysia

Occurrence and antibiotic resistance of Salmonella spp. in raw beef from wet market and hypermarket in Malaysia

High Occurrence of Extended-Spectrum β-Lactamase-ProducingSalmonellain Broiler Carcasses from Poultry Slaughterhouses in South Korea

Evaluation of the Performance of Two Selective Enrichment Media and Two Selective Plating Media for the Detection of <i>Salmonella</i> from Primary Poultry Production, According to ISO 6579:2002

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Evaluation of selective enrichment broths and chromogenic media for Salmonella detection in highly contaminated chicken carcasses

Cited by 11 publications

References 29 publications

Occurrence and antibiotic resistance of Salmonella spp. in raw beef from wet market and hypermarket in Malaysia

Occurrence and antibiotic resistance of Salmonella spp. in raw beef from wet market and hypermarket in Malaysia

High Occurrence of Extended-Spectrum β-Lactamase-ProducingSalmonellain Broiler Carcasses from Poultry Slaughterhouses in South Korea

Evaluation of the Performance of Two Selective Enrichment Media and Two Selective Plating Media for the Detection of &lt;i&gt;Salmonella&lt;/i&gt; from Primary Poultry Production, According to ISO 6579:2002

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Evaluation of the Performance of Two Selective Enrichment Media and Two Selective Plating Media for the Detection of <i>Salmonella</i> from Primary Poultry Production, According to ISO 6579:2002