Evaluation of serological transfusion-transmitted viral diseases and mutliplex nucleic acid testing in malaysian blood donors

Yaseen, Saif; Ahmed, Suhair Abbas; Johan, Muhammad Farid; Kiron, R.; Daher, Aqil Mohammad

doi:10.1016/j.transci.2013.07.003

Cited by 16 publications

(13 citation statements)

References 24 publications

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“…In the present study, the HCV positive donations were 1:1698, which is much higher than studies done in the USA and Asia. NAT yield in the present study was even higher than the rate previously reported in similar studies conducted in Egypt (14,15).…”

Section: Discussioncontrasting

confidence: 73%

Optimization of Pooling Technique for Hepatitis C Virus Nucleic Acid Testing (NAT) in Blood Banks

et al. 2020

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Background: Blood safety is an important health issue, especially in Egypt with a high prevalence of HCV infection and recent massive efforts for control. Objectives: The study was conducted to evaluate the feasibility of the nucleic acid amplification technique (NAT) in Egyptian blood banks. Methods: A total of 12,036 plasma sample donations collected from two Egyptian blood banks were screened by NAT using a chessboard pooling scheme. The donations were divided into two groups: Group 1 included 10,020 seronegative, while Group 2 included 2,016 blindly tested donations. Results: Out of the total donations, seven tests were positive by NAT. The serology results of those seven seronegative donations were confirmed. They were also retested individually by NAT. The pool format 6x12 was the most reliable for screening. The cost of NAT was reduced to one-quarter and the positive sample in the pool was easily pointed by using the chessboard format. Conclusions: The study demonstrated the importance of introducing NAT for HCV screening in Egyptian blood banks by adopting a pooling scheme to reduce cost.

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Section: Discussioncontrasting

confidence: 73%

Optimization of Pooling Technique for Hepatitis C Virus Nucleic Acid Testing (NAT) in Blood Banks

et al. 2020

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“…HCV RNA in infected samples can be detected within one week of initial exposure 19 . Due to high sensitivity and specificity, NAT is used for the resolution of samples which are reported as indeterminate and or false negative by serological methods 85 .…”

Section: Diagnostic Methods For Hcvmentioning

confidence: 99%

Genotyping & diagnostic methods for hepatitis C virus

Kumar¹,

Rajput²,

Paliwal³

et al. 2018

Indian Journal of Medical Research

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Hepatitis C virus (HCV) infection is a blood borne and transfusion-transmitted infection (TTI). It has emerged as one of the major health challenges worldwide. In India, around 12-18 million peoples are infected with HCV, but in terms of prevalence percentage, its looks moderate due to large population. The burden of the HCV infection increases due to lack of foolproof screening of blood and blood products before transfusion. The qualified screening and quantification of HCV play an important role in diagnosis and treatment of HCV-related diseases. If identified early, HCV infection can be managed and treated by recently available antiviral therapies with fewer side effects. However, its identification at chronic phase makes its treatment very challenging and sometimes ineffective. The drugs therapy for HCV infection treatment is also dependent on its genotype. Different genotypes of HCV differ from each other at genomic level. The RNA viruses (such as HCV) are evolving perpetually due to interaction and integration among people from different regions and countries which lead to varying therapeutic response in HCV-infected patients in different geographical regions. Therefore, proper diagnosis for infecting virus and then exact determination of genotype become important for targeted treatment. This review summarizes the general information on HCV, and methods used for its diagnosis and genotyping.

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“…Asian countries for example have intermediate or highly endemic HBV infection where 16-90% of adults had either past or ongoing HBV infections [25]. A study by a Malaysian group showed that 1,388 donor samples tested by serology methods were in fact non-reactive by NAT [26]. These samples were confirmed to be "false-reactive" on confirmatory serological tests.…”

Section: Province Location Sample Size Hbsag Anti-hbc Anti-hcv Anti-hmentioning

confidence: 99%

Prevalence and Trends of Common Transfusion Transmitted Infections Using Serological and Nucleic Acid Markers in Saudi Blood Donors

2015

J Blood Disord Transfus

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Background: Epidemiologic studies on the prevalence of transfusion-transmitted infections (TTIs) in Saudi Arabia (SA) regions are limited. This study investigated the prevalence of HBV, HCV, and HIV using both serological and nucleic acid testing (NAT) methods to determine temporal and geographic trends among blood donors in Makkah. Our secondary objective was to realize the most suitable NAT format, without compromising sensitivity of NAT results by using individualized or mini-pool testing.

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Evaluation of serological transfusion-transmitted viral diseases and mutliplex nucleic acid testing in malaysian blood donors

Cited by 16 publications

References 24 publications

Optimization of Pooling Technique for Hepatitis C Virus Nucleic Acid Testing (NAT) in Blood Banks

Optimization of Pooling Technique for Hepatitis C Virus Nucleic Acid Testing (NAT) in Blood Banks

Genotyping & diagnostic methods for hepatitis C virus

Prevalence and Trends of Common Transfusion Transmitted Infections Using Serological and Nucleic Acid Markers in Saudi Blood Donors

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