Evaluation of serum exosomal LncRNA‐based biomarker panel for diagnosis and recurrence prediction of bladder cancer

Abstract

Exosomes are small membrane vesicles released by many cells. These vesicles can mediate cellular communications by transmitting active molecules including long non‐coding RNAs (lncRNAs). In this study, our aim was to identify a panel of lncRNAs in serum exosomes for the diagnosis and recurrence prediction of bladder cancer (BC). The expressions of 11 candidate lncRNAs in exosome were investigated in training set (n = 200) and an independent validation set (n = 320) via quantitative real‐time PCR. A three‐lncRNA panel (PCAT‐1, UBC1 and SNHG16) was finally identified by multivariate logistic regression model to provide high diagnostic accuracy for BC with an area under the receiver‐operating characteristic curve (AUC) of 0.857 and 0.826 in training set and validation set, respectively, which was significantly higher than that of urine cytology. The corresponding AUCs of this panel for patients with Ta, T1 and T2‐T4 were 0.760, 0.827 and 0.878, respectively. In addition, Kaplan‐Meier analysis showed that non‐muscle‐invasive BC (NMIBC) patients with high UBC1 expression had significantly lower recurrence‐free survival (P = 0.01). Multivariate Cox analysis demonstrated that UBC1 was independently associated with tumour recurrence of NMIBC (P = 0.018). Our study suggested that lncRNAs in serum exosomes may serve as considerable diagnostic and prognostic biomarkers of BC.

Cancer‐associated fibroblasts–derived exosomes‐mediated transfer of LINC00355 regulates bladder cancer cell proliferation and invasion

“…Studies have found that long noncoding RNAs (lncRNAs) can be expressed in exosomes derived from tumor serum, cells, and microenvironment . Studies have shown that serum and urine exosome lncRNA can be used as biomarkers for the diagnosis and prognosis of BC . In addition, exosomal lncRNA is also involved in the regulation of BC development .…”

Identification of five long noncoding RNAs signature and risk score for prognosis of bladder urothelial carcinoma

“…1,4 Hence, great attention is required for early diagnosis and prognosis prediction. 27 In addition, there is growing evidence that lncRNA might act as prognostic indicators in BLCA development and progression. 25,26 Nowadays, a set of studies elucidated that lncRNAs exerted a lot in the pathogenesis of BLCA.…”

Abnormally expressed long noncoding RNA B3GALT5‐AS1 may serve as a biomarker for the diagnostic and prognostic of gastric cancer

“…25 Similarly, Lee et al validated the importance of serum exosomal ncRNAs (miRNA-21 and lncRNA-ATB) in the diagnosis and prognosis monitoring of human HCC. qRT-PCR, reverse transcription quantitative real-time polymerase chain reaction group and the control group was 0.857 and 0.826.…”