Evaluation of serum levels of p53 in hepatocellular carcinoma in Egypt

Far, Mohamed A. El; Atwa, Mohamed; Yahya, Raida S.; Basuni, Mohamed A. El

doi:10.1515/cclm.2006.091

Cited by 20 publications

(9 citation statements)

References 12 publications

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“…Serum anti-p53 [22,23] levels have been associated with survival of HCC patients, tumor size and tumor grade. Similarly, overexpression of p53 has correlated with overall survival rates of HCC patients [24][25][26] , tumor size and grade [22] . Thus, both serum p53 and anti-p53 have been suggested as biomarkers complementary to AFP [27][28] .…”

Section: R=04564 P=00051mentioning

confidence: 99%

P53 Inducible Gene-3: Potential Serum Marker for Hepatocellular Carcinoma Beyond Milan Criteria

Bhat

Goldstein

Ali

et al. 2015

Journal of Gastroenterology and Hepatology Research

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AIMS: The diagnosis of Hepatocellular carcinoma (HCC) is usually made late, precluding curative resection or liver transplantation in over 70% of HCC patients. P53 inducible gene 3 (PIG3) is a p53 downstream gene and a key component of the DNA damage response pathway, which is relevant in conditions with rapid cell turnover such as HCC. Our aim was to assess whether PIG3 could serve as a novel biomarker for HCC. METHODS: A pilot study of 37 consecutive patients with the diagnosis of HCC, 42 patients with hepatic cirrhosis versus healthy controlswas conducted. Serum AFP and PIG3 levels measured with enzyme-linked immunosorbent assay were compared across groups using student t-testand correlation coefficient (R) between AFP and PIG3 was calculated. RESULTS: Clinical characteristics were similar across the HCC and cirrhosis groups. Serum PIG3 levels were significantly higher among patients with cirrhosis or HCC (23.27±13.26 and 24.1±18.61 ng/ mL, respectively), as compared to healthy controls (3.71±1.79 ng/ mL, p<0.0001). PIG3 values were significantly different across the following tumor diameter ranges: less than 2 cm, 18.47 ng/mL; 2 to 5 cm, 18.54 ng/mL; and greater than 5 cm, 38.72 ng/mL; p=0.0012. The correlation coefficient between log (AFP) and PIG3 levels in HCC patients was r=0.4564, p=0.005. CONCLUSION: Serum p53-inducible gene 3 levels were significantly elevated in patients with HCC beyond 5 cm, indicating potential as an adjunct to imaging in determining eligibility for liver transplantation. The potential for PIG3 as a biomarker of HCC warrants further prospective study.

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Section: R=04564 P=00051mentioning

confidence: 99%

P53 Inducible Gene-3: Potential Serum Marker for Hepatocellular Carcinoma Beyond Milan Criteria

Bhat

Goldstein

Ali

et al. 2015

Journal of Gastroenterology and Hepatology Research

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“…Similarly, “informal care” dispensed by traditional healers has been associated with an increased risk of anti‐HCV seropositivity in countries neighbouring the Maghreb like Egypt [Habib et al, ; El et al, ]. Such “healers” are still in active in Maghreb countries (especially in Morocco) and may have played a role in the maintenance and spread of HCV within North African populations.…”

Section: Epidemiology Of Hepatitis C In the Maghreb Regionmentioning

confidence: 99%

Hepatitis C virus infection in the Maghreb region

Ezzikouri

Pineau

Benjelloun

2013

Journal of Medical Virology

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Hepatitis C is a global health problem with a worldwide prevalence of about 3% (around 170 million people). Hepatitis C virus (HCV) is major concern in the Maghreb countries, Algeria, Libya, Mauritania, Morocco, and Tunisia, but no detailed description of its epidemiology in the region is available. In the present review, a systematic search was undertaken covering HCV data available in peer-reviewed databases as well as institutional reports and regional conference meeting abstracts from the Maghreb countries. Reports written in English and French were included in this analysis. Estimates of national and regional prevalence of HCV infection (based on anti-HCV antibody) and of the size of patient populations were performed. In addition, the molecular features of the circulating viral strains in the region are discussed. A substantial proportion, 1.2-1.9% of the Maghreb inhabitants, have anti-HCV antibodies. Genotype 1b predominates among viral strains in all countries except in Libya, where genotype 4 is dominant as in neighboring Egypt. This epidemiological situation is of significant concern, and requires urgent, broad, and active intervention for the prevention and control of HCV. More specifically, the application of state-of-the-art hygiene procedures and rigorous controls in medical disciplines such as hemodialysis, transfusion, endoscopic procedures, and dentistry is necessary to reduce significantly the number of new infections in the region.

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“…All these experiments indicated that AMSNs could be used as an efficient gene transfer vector. There was evidence that the level of p53 alterations was high in HCC and p53 increased the frequency of HCC prediction from 79.5 to 86.3% [26]. Activation of Fig.…”

Section: Discussionmentioning

confidence: 90%

Novel amino-modified silica nanoparticles as efficient vector for hepatocellular carcinoma gene therapy

Xiao

Huang

2009

Med Oncol

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Due to the ineffective conventional treatment for hepatocellular carcinoma (HCC), the nonviral gene delivery system has been proved to be an attractive alternative to HCC therapy. In this work, we have developed a kind of new self-assembled nanoparticles, which were named as amino-modified silica nanoparticles (AMSNs). Scanning electron microscopy and zeta potential results demonstrated that AMSNs had a diameter of 20-30 nm and positive surface charges of +11.3 mV, respectively. The AMSNs could bind DNA strongly and protect DNA from degradation, which was confirmed by DNA-binding assay and serum protection assay. Furthermore, AMSNs could transfer foreign DNA into targeted cells with high transfection efficiency and little cytotoxicity. Combined with the p53 gene, AMSNs could transfect pp53-EGFP in HepG2 cells and result in a high-level of p53 mRNA and protein expressions. The nude mice treated with AMSNs/pp53-EGFP complexes showed significant tumor growth inhibition. Our results showed the AMSNs, an efficient gene vector, had the potential of gene therapy for HCC.

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Evaluation of serum levels of p53 in hepatocellular carcinoma in Egypt

Cited by 20 publications

References 12 publications

P53 Inducible Gene-3: Potential Serum Marker for Hepatocellular Carcinoma Beyond Milan Criteria

P53 Inducible Gene-3: Potential Serum Marker for Hepatocellular Carcinoma Beyond Milan Criteria

Hepatitis C virus infection in the Maghreb region

Novel amino-modified silica nanoparticles as efficient vector for hepatocellular carcinoma gene therapy

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