Evaluation of seven X-chromosomal short tandem repeat loci located within the Xq26 region

Rodig, Heike; Kloep, Frank; Weißbach, Lydia; Augustin, Christa; Edelmann, Jeannett; Hering, Sandra; Szibor, R.; Götz, Frank; Brabetz, Werner

doi:10.1016/j.fsigen.2009.08.010

Cited by 22 publications

(15 citation statements)

References 19 publications

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“…We also typed the present 10-X-STRs for control reference sample 9947A to compare our results with those of panel cells. All of the allele types were identical to those described at X-STR org or in other reports [4,6] except that for DXS6799.…”

Section: Construction Of Decaplex Pcr System and Allele Designationmentioning

confidence: 99%

“…Although the STR combination in group 6 spans distances of as much as 140 kb, no significant linkage disequilibrium was observed in the present Japanese samples. The trio STR clusters of DXS6801-DXS6809-DXS6789, DXS10079-DXS10074-1DXS10075, DXS10146-DXS10134-DXS10147, DXS10148-DXS10135-DXS8378, and DXS10103-HPRTB-DXS10101 have been proposed as haplotypes [1][2][3][4]6]. These STRs were studied further in other populations [7][8][9].…”

Section: Linkage Equilibrium Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…The primer sequences, concentrations used in the multiplex, type of labeling dye, and range of amplified fragment sizes are listed in Table 1. Sequences of primers were obtained from X-STR org (http://www.chrx-str.org) and other studies [4,6,11] but were partly changed except for one sequence depending on the profile of the multiplex electrophoretogram. Multiplex PCR was performed in a volume of 20 μl reaction mix containing: 3 ng genomic DNA, 1×10 mM Tris-HCl at pH 8.3, 50 mM KCl, 2.5 mM MgCl2, 0.001% gelatin, 200 μM dNTP, 1.75 U AmpliTaq Gold (Applied Biosystems), and an appropriate volume of each primer ( Table 1).…”

Section: Introductionmentioning

confidence: 99%

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Population genetic study of six closely linked groups of X-STRs in a Japanese population

2011

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X chromosome STR (X-STR) polymorphisms are a useful tool in the fields of human population genetics and personal identification and are quite informative in the investigation of complex kinship or deficiency cases, especially where it is necessary to determine relationships with second-generation offspring in which the same X chromosome may have been inherited. We investigated eight X-STR systems using the Mentype Argus X-8 kit and further developed decaplex PCR for the DXS10148, DXS10161, DXS10160, DXS10159, DXS10079, DXS10075, DXS6799, DXS10102, DXS10106, and DXS10146 loci with the aim of constructing closely linked groups on the X chromosome. The studied population comprised 569 Japanese individuals (390 males and 179 females). Heterozygosity among the present 18 X-STRs showed a distribution of from 54.2% to 90.5%. We constructed six closely linked groups, each comprising three to five X-STRs: DXS10148-DXS10135-DXS8378, DXS10161-DXS10160-DXS10159, DXS7132-DXS10079-DXS10074-DXS10075-DXS981, DXS6809-DXS6789-DXS6799, DXS10102-HPRTB-DXS10101-DXS10106, and DXS8377-DXS10146-DXS10134-DXS7423. The forensic utility of these groups as haplotypes was then evaluated. Haplotype diversity values showed a distribution of from 0.9699 to 0.9959. Analysis of the present closely linked haplotypes will contribute to solving complex kinship cases involving X chromosome inheritance.

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Section: Construction Of Decaplex Pcr System and Allele Designationmentioning

confidence: 99%

Section: Linkage Equilibrium Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Population genetic study of six closely linked groups of X-STRs in a Japanese population

2011

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“…The human X chromosome has been the focus of much research in the fields of population genetics and forensics in recent years, and closely linked groups of markers are becoming more attractive [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15]. X-chromosomal short tandem repeats (X-STRs) can be used to complement autosomal STRs in paternity testing in female children.…”

Section: Introductionmentioning

confidence: 99%

Genetic study of 12 X-STRs in Malay population living in and around Kuala Lumpur using Investigator Argus X-12 kit

et al. 2012

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We investigated 12 X-chromosomal short tandem repeat (STR) polymorphisms in 283 unrelated Malay individuals (160 males and 123 females) living in and around Kuala Lumpur using the Investigator Argus X-12 kit. Heterozygosity among the present 12 X-STRs showed a distribution of from 55.3 to 93.5 %. The diversity values of the haplotypes constructed using four closely linked groups were all higher than 0.9865. A comparison of allelic frequency in each system and haplotype variation indicated that the nature of these X-STRs in the Malay population differed from that in East Asian, European, or African populations. Several microvariant alleles found in the Malay population were characterized and compared with known sequence data. The present data may be helpful in forensic casework such as personal identification and kinship testing in the Malay population in Malaysia.

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Development and validation of a new 18 X‐STR typing assay for forensic applications

Zhang

et al. 2021

Electrophoresis

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With a unique inheritance pattern compared to autosomal short tandem repeats (A‐STRs), X chromosomal STRs (X‐STRs) have special usage in forensic relationship testing. In this study, we designed a multiplex amplification system (named TYPER‐X19 multiplex assay) consisting of 18 STR loci spreading from 7.837 to 149.460 Mb on the X chromosomes (DXS9895, DXS8378, DXS9902, DXS6810, DXS7132, DXS10079, DXS6789, DXS7424, DXS101, DXS6797, DXS7133, DXS6804, GATA165B12, DXS10103, HPRTB, GATA31E08, DXS8377, and DXS7423), and the amelogenin. PCR primers were marked with four kinds of fluorophores including FAM, HEX, TAMRA, and ROX. The multiplex system was optimized and tested for precision, concordance, reproducibility, sensitivity, stability, DNA mixture, and species specificity according to the conventional validation guidelines. The results indicated that the system was accurate, reliable, and sensitive enough, and was suitable for common forensic case‐type samples. In the population genetic study, a total of 148 alleles were detected at the 18 X‐STR loci in 398 Southern Han Chinese. Relatively high combined power of discrimination in male (PDm), power of discrimination in female (PDf), mean paternity exclusion chance in trios (MECtrio), and mean paternity exclusion chance in duos (MECDuo) by Desmarais were detected, and HPRTB‐DXS10103 was in linkage disequilibrium. The results suggested that the TYPER‐X19 multiplex assay was suitable for forensic applications.

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Evaluation of seven X-chromosomal short tandem repeat loci located within the Xq26 region

Cited by 22 publications

References 19 publications

Population genetic study of six closely linked groups of X-STRs in a Japanese population

Population genetic study of six closely linked groups of X-STRs in a Japanese population

Genetic study of 12 X-STRs in Malay population living in and around Kuala Lumpur using Investigator Argus X-12 kit

Development and validation of a new 18 X‐STR typing assay for forensic applications

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