Evaluation of several adjuvants in avian influenza vaccine to chickens and ducks

Abstract

The effects of three different adjuvants, mineral oil, Montanide™ ISA 70M VG, and Montanide™ ISA 206 VG, were evaluated on reverse genetics H5N3 avian influenza virus cell cultured vaccine. The immune results of SPF chickens after challenging with highly pathogenic avian influenza (HPAI) virus demonstrated that mineral oil adjuvant group and 70M adjuvant group provided 100% protection efficiency, but 206 adjuvant group provided only 40%. Statistical analysis indicated that the protection effects of mineral oil adjuvant group and the 70M adjuvant showed no significant difference to each other, but with significant difference to 206 adjuvant group. All three groups could induce high titres of antibody after immunizing SPF ducks, but there was no significant difference among them. The immunization effect of 70M adjuvant group on SPF chickens were the best and showed significant difference compared with optimized 70Mi Montanide™ eight series adjuvants groups. These results suggest that 70M adjuvant could be a novel adjuvant for preparing avian influenza vaccine.

A user-friendly and scalable process to prepare a ready-to-use inactivated vaccine: The example of heartwater in ruminants under tropical conditions

“…ISA70+ER vaccine was also able to induce a good protection with a 80% survival rate. During the vaccination trial (at primary and boost injections), we also observed that ISA70M-based vaccines were easily injected and well tolerated by the animals, with no major side effects, as previously observed [28].…”

“…ISA70 and/or ISA70M adjuvants have already proven to induce strong humoral and cellular protective immune responses [28,29]. Herein, we observed a strong MAP-1B antibody response in animals vaccinated with all adjuvants.…”

Inactivated vaccine with adjuvants consisting of pattern recognition receptor agonists confers protection against avian influenza viruses in chickens

“…Vaccination is one of the primary strategies to prevent and control these infectious diseases spread in poultry industry in China and other countries (Swayne, 2012). However, influenza viruses with antigenic mutations in the hemagglutinin (HA) gene may escape host immunity elicited by the vaccine strain, and cause outbreaks (Connie Leung et al, 2013; Liu et al, 2011a; Sun et al, 2012). Furthermore, the pivotal steps of the traditional vaccine development processes involving identification of antigenicity match of the vaccine strain to the predicted circulating viruses in the coming season are time-costing and labor-consuming procedures (Sun et al, 2012).…”

Efficacy of Montanide ISA-70-VG as Adjuvant to Fowl Cholera Vaccine

“…Oil emulsion vaccine could promote antibody titer with prolonged immunity. However mineral oil long term stand at the site of injection causing inflammation and local tissue necrosis leading to lower commercial value of birds (Chun et al, 2011).…”