The cultivation and breeding of new pear tree varieties have great potential, especially with the changing climate. The introduction of genetic resources and varieties from regions with a high genetic diversity can have a positive effect on the cultivation of pear trees in our region. In this experiment, a total of 23 Asian pear varieties originating from Japan and China were assessed. Based on the values obtained, the varieties were sorted, and suitable varieties for the conditions of the Czech Republic were selected. The early-maturing varieties that proved to be promising were ‘Shinseiki’ and ‘Zao Su Li’ due to their refreshing juiciness, plasticity and ease of cultivation. generally low demand. The ‘Shinseiki’ bears medium- to small-sized fruits (103 g on average). The size of the fruits of the evaluated cultivar ‘Zao Su Li’ can reach very large sizes (255 g on average). The medium-maturity varieties suitable for the conditions of the Czech Republic were ‘Hosui’ and ‘Dangshansu Li’ (ripening on the September 11–19 on average) and another interesting variety, ‘Kirgizkaja zimnaja’. Other suitable medium-maturity varieties are ‘Nijisseiki’, ‘Sha Li’ and ‘Pung Su’. Of the assessed late-maturing varieties, the promising ones were ‘Ya Li’ and ‘Mut Chen’, especially due to their relatively high storability under cold storage.