Evaluation of Simplified Antipyrine Test in Calves

Abstract

The purpose of this study was to test a modification of antipyrine test in calves. The experiment was carried out on 10 calves (bulls of black and white breed). Each subject received on days 10 and 20 of life. respectively. 15 mg.kg· l body mass of antipyrine as an intravenous bolus injection. followed by its oral administration "in substantia" in gelatin capsules 48 hours later. The concentrations of antipyrine in their saliva were repeatedly determined. Pharmacokinetics of antipyrine was followed in each subject (according to a one-compartment open model) using five modifications: a complete study (A). and four abbreviated studies. i.e using the 4. 8 and 12 h saliva collection times (B). the 4 and 8 h collection times (C), further the 4 and 12 h (D). and finally the 8 and 12 h saliva collection times (E). Correlational analysis was used to compare the substance kinetics from the complete set of data obtained from all saliva collections with the results using each of the abbreviated approaches.Based on the complete kinetics studies, mean variables for antipyrine were as follows: half-life In conclusion. we demonstrated that antipyrine pharmacokinetic parameters in calves can be determined with reasonable precision using a two-point or three-point saliva sampling procedure following a single oral dose. Moreover. this simplified sampling method can be used for largescale investigations. where estimation of hepatic mixed function oxygenases (MFO) activity is needed.Antipyrine, intravenous and oral administration. MFO system, calves

Effect of starvation for four days on antipyrine metabolism in calves

“…The renal clearance of unchanged antipyrine slightly decreased after starvation for four days. The results from previous experiments reported by the authors (J anus et al 1997) indicated that renal excretion and clearance of unchanged antipyrine in calves was practically independent of diuresis and (generally) renal function.…”