Evaluation of skin anti-aging potential of Citrus reticulata blanco peel

Abstract

Background:The peel of Citrus reticulata Blanco is traditionally used as tonic, stomachic, astringent, and carminative. It is also useful in skin care.Objective:To study the anti-aging potential of alcoholic extracts of C. reticulata Blanco peel using in vitro antioxidant and anti-enzyme assays.Materials and Methods:Plant extracts were obtained by Soxhlation (CR HAE- Hot Alcoholic Extract of Citrus reticulata) and maceration method (CR CAE- Cold Alcoholic Extract of Citrus reticulata). Qualitative and quantitative phytochemical analysis was performed. Further, in vitro antioxidant, anti-enzyme, and gas chromatography-mass spectrometry (GC-MS) analyses were performed.Results:Total phenolic and flavonoid contents of CR HAE were found to be higher than CR CAE. EC50 value of CR HAE and CR CAE for 1,1-Diphenyl-2-picrylhydrazyl, Superoxide anion, and 2, 2’-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) assays were 250.33 ± 40.16 μg/ml and 254.73 ± 15.78 μg/ml, 221.27 ± 11.25 μg/ml and 354.20 ± 23.79 μg/ml, and 59.16 ± 2.17 μg/ml and 59.12 ± 6.21 μg/ml, respectively. Oxygen radical absorbance capacity values for CR HAE and CR CAE were found to be 1243 and 1063 μmoles 6-hydroxy-2,5,7,8-tetra methylchromane-2-carboxylic acid equivalent/g of substance, respectively. Anti-collagenase and anti-elastase activities were evaluated for both CR HAE and CR CAE. EC50 values of CR HAE and CR CAE for anti-collagenase and anti-elastase were 329.33 ± 6.38 μg/ml, 466.93 ± 8.04 μg/ml and 3.22 ± 0.24 mg/ml, 5.09 ± 0.30 mg/ml, respectively. CR HAE exhibited stronger anti-collagenase and anti-elastase activity than CR CAE. GC-MS analysis of CR HAE was carried out because CR HAE exhibited higher antioxidant and anti-enzyme potential than CR CAE.Conclusion:C. reticulata peel can be utilized in anti-wrinkle skin care formulations.SUMMARY Skin anti-aging potential of Citrus reticulata Blanco peel was evaluated throughIn vitro antioxidant and anti-enzyme assaysTwo types of extraction were performed and extracts were subjected to qualitative and quantitative phytochemical analysis. Extract obtained by Soxhlation (CR HAE) showed higher total phenolic and flavonoid contents than extract obtained by maceration (CR CAE)CR HAE demonstrated strong DPPH and Superoxide free radical scavenging activity whereas, ABTS scavenging activity of both the extracts were found to be similar. Oxygen Radical Absorbance Capacity (ORAC) of CR HAE was found to be more; indicating its strong antioxidant potentialIn vitro collagenase and elastase enzyme inhibition activities were evaluated for both the extracts and CR HAE showed strong anti-collagenase and antielastase potential indicating its anti-aging abilityGC-MS analysis of CR HAE revealed the presence of various compounds mainly including Polymethoxyflavones. CR HAE exhibited promising antioxidant and anti-enzymatic activity and can be used as a potent antiwrinkle agent in anti-aging skin care formulations. Abbreviation Used: ECM: Extracellular matrix, UV: Ultra violet, ROS: Reactive Oxygen Species, MMP: Matrix ...

Inhibition of UVB‐induced pro‐inflammatory cytokines and MMP expression by Zanthoxylum rhetsa bark extract and its active constituent hesperidin

“…These results suggested that the chemical constituents in the extract may have antiaging properties via inhibiting elastase and delaying the degradation of elastin fibers. Previous studies reported that the plant extract and phytocompounds with good elastase inhibition activity such as white tea (89%), Citrus reticulate (80%), epigallocatechin gallate (84.79%), oleanolic acid (74.1%), and catechin (80.5%) can offer significant antiaging properties (Apraj & Pandita, ; Thring et al, ).…”

Gold lotion from citrus peel extract ameliorates imiquimod‐induced psoriasis‐like dermatitis in murine

“…Gold lotion (GL), a formulated product extracted from the peels of six citrus fruits (navel oranges, Citrus hassaku , Citrus limon , Citrus natsudaidai , Citrus miyauchi Iyo and Satsuma), has been developed in Japan. GL contains various flavonoids that have potential to protect the skin against UV‐induced damage and aging . In addition to skin protection, GL has also been demonstrated to suppress azoxymethane‐induced aberrant crypt foci formation in mice by downregulating inflammation‐related protein levels that may subsequently prevent inflammation‐associated colon tumorigenesis .…”

Antioxidant and Anti-Collagenase Activity of Sargassum Plagyophyllum Extract as an Anti-Wrinkle Cosmetic Ingredient

“…23,24 One of the most common method for testing preliminary antioxidant activity of plant extracts is DPPH radical-scavenging. 25 In this test, the antioxidant activity of S. plagyophyllum extract (50 mg/mL) was 41,61±0,02% (E1), 39,16±0,01% (E2), 37,58±0,03% (E3) and ascorbic acid 78.03±0,65 % (22.44 µg/mL) as antioxidant activity comparison (Figure 3.). Extract ethanol 25% of S. plagyophyllum had the highest antioxidant activity than other extract.…”