Evaluation of sodium dichloroisocyanurate treatment on recovered concentrations of Salmonella enterica, Escherichia coli O157:H7, and Listeria monocytogenes from cattle hide surfaces and culture medium

Abstract

A major concern of the cattle industry is cross-contamination of meat with pathogens. Cattle are exposed to fecal material, mud, and other contaminants which harbor pathogens that can be shed onto meat and meat processing equipment. Due to increased chances of meat contamination during processing, new antimicrobial formulations for carcass washing before hide removal needs to be identified and tested. Sodium dichloroisocyanurate (SDIC) has biocidal properties and belongs to the Nhalamine group of compounds. Disk diffusion assays revealed, 1,000 ppm SDIC effectively reduced pathogen concentrations. SDIC was evaluated for its effects on pathogens in Tryptic Soy Broth and results revealed that 1,000 ppm SDIC had a strong correlation with time and treatment with no bacterial growth in log CFU ml −1 observed at the lowest detection level. Treatment of inoculated hides with 1,000 ppm SDIC for 5 min resulted in reduction of Salmonella enterica, Escherichia coli O157:H7, and Listeria monocytogenes at 1.97, 2.02, and 2.84 log CFU cm −2 , respectively.

Efficacy of Fatty Acid Amide Derivatives against Listeria monocytogenes

“…The fatty acid amide derivatives were synthesized by activating the carboxylic acid using thionyl chloride followed by reacting the acid chloride with pyrrolidine or piperidine amines (The synthetic procedure is included in the supporting information). Solution test was performed to assess the antibacterial efficacy of the synthesized fatty acid amides against the cocktail of four Listeria monocytogenes (LMC) strains and the effectiveness was determined by calculating the log reduction in bacterial count as reported in the literature [42–46] …”