Antagonistic fungi naturally occurring on faba bean leaf surface were isolated and evaluated for their activity as bioagents for Botrytis fabae the causative agent of chocolate spot disease. Thirty isolates were purified and identified as 26 isolates of Trichoderma species (Trichoderma album, T. aureoviride , T. hamatum, T. harzianum and T. viride) and 4 isolates belonging to the genera of Cladosporium, Gliocladium, Epicoccum and Paecilomyces. The inhibitory effect of these isolates was assessed in vitro against the growth of B. fabae, which decreased it's mycelial growth on PDA plates. The inhibitory effect of Trichoderma spp. ranged between 51.11 -77.78%. In addition, T. album (Isolate 2) gave the highest inhibition followed by T. harzianum (Isolate 6). Furthermore, under greenhouse conditions spraying of faba bean plants with any of Trichoderma spp. and Bio-Zeid as a biofungicide, 24 h before inoculation with B. fabae significantly reduced the severity of the disease after 14 days in the range of 3.0 -4% compared with the control (8.7%). T. album (Isolate 2) was the highest antagonistic isolate (3.0%) followed by T. harzianum (Isolate 6) then T. hamatum (Isolate 6) and T. viride (Isolate 2), being 3.24, 3.30 and 3.40%, respectively. Volatile and non-volatile compounds produced by T. album (Isolate 2) exhibited the highest inhibition to the mycelial growth of B. fabae followed by T. harzianum (Isolate 6).