Evaluation of some therapies and meristem culture to eliminate Potato Y potyvirus from infected potato plants

Al-Maarri, K.; Massa, Rima; Al-Biski, F.

doi:10.5511/plantbiotechnology.12.0215a

Cited by 28 publications

(24 citation statements)

References 17 publications

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“…This procedure resulted in about 53% survival of the heat-treated shoots, 56% regeneration of shoot tips, and 91% and 74% of GLRaV-1- and GRSPaV-1-free plants. Thermotherapy followed by shoot tip culture was reported to eradicate PVY from infected potato [ 29 ]. In vitro virus-infected potato shoots were thermo-treated for 40 days at a consistent temperature of 37 °C, followed by shoot tip culture (0.1–0.3 mm).…”

Section: Thermotherapy-based Methods For Virus Eradicationmentioning

confidence: 99%

“…In vitro virus-infected potato shoots were thermo-treated for 40 days at a consistent temperature of 37 °C, followed by shoot tip culture (0.1–0.3 mm). Culture of 0.1 mm shoot tips resulted in about 88% survival levels of the treated shoots and 75–81% virus-free plants in two potato cultivars, and larger shoot tips decreased the virus eradication frequencies [ 29 ].…”

Section: Thermotherapy-based Methods For Virus Eradicationmentioning

confidence: 99%

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In vitro thermotherapy-based methods for plant virus eradication

et al. 2018

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Production of virus-free plants is necessary to control viral diseases, import novel cultivars from other countries, exchange breeding materials between countries or regions and preserve plant germplasm. In vitro techniques represent the most successful approaches for virus eradication. In vitro thermotherapy-based methods, including combining thermotherapy with shoot tip culture, chemotherapy, micrografting or shoot tip cryotherapy, have been successfully established for efficient eradication of various viruses from almost all of the most economically important crops. The present study reviewed recent advances in in vitro thermotherapy-based methods for virus eradication since the twenty-first century. Mechanisms as to why thermotherapy-based methods could efficiently eradicate viruses were discussed. Finally, future prospects were proposed to direct further studies.

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Section: Thermotherapy-based Methods For Virus Eradicationmentioning

confidence: 99%

Section: Thermotherapy-based Methods For Virus Eradicationmentioning

confidence: 99%

In vitro thermotherapy-based methods for plant virus eradication

et al. 2018

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“…Effects of different antiviral chemicals including 2-thiouracil on PLRV elimination from In vitro-grown potato was reported and found that ribavirin and 2-thiouracil are effective for the production of potato virus free plantlets (Singh 2015). 2-thiouracil has also been used during virus elimination in plum plants and found that this antiviral drug has elimination capacity on plum pox virus (PPV) and a number of plants can be regenerated during eradication process (AlMaarri et al 2012;Jakab-Ilyefalvi et al 2012). Dithioracil can be combined with ribavirin for cymbidium mosaic virus elimination in dendrobium orchid (Katrina and Adelheid 1997).…”

Section: Antiviral Activity Of Other Antiviral Reagents For Eradicatingmentioning

confidence: 99%

Major In Vitro Techniques for Potato Virus Elimination and Post Eradication Detection Methods. A Review

2019

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Potato is an economically important agro-industrial crop that is conventionally propagated, however; its potential transmission of viruses through seed tubers from generation to generation is a major limitation of potato yield production. In order to produce potato virus-free and sufficient amount of potato seed tubers, several approaches of in vitro methods for virus elimination have been developed. Meristem culture has been used alone or combined with techniques such as thermotherapy, electrotherapy, cryotherapy and chemotherapy as the best alternative method for treating potato infected by viruses. Recent literature has shown that to eliminate potato virus significantly depends upon the potato cultivar, antiviral agents, type of virus, the duration of heat treatment. Appropriate duration for efficiency elimination is still under investigation. Viral elimination rate can be detected through serological methods such as enzyme-linked immunosorbent assay (ELISA) and molecular biology technique such as real time reverse transcriptase polymerase chain reaction (real time RT-PCR) that are used for pre and post elimination virus detection to evaluate the efficiency and the accuracy of virus elimination method. The purpose of this review is to highlight virus elimination methods in potato and recommending the most effective tool for virus detection in order to ensure the production of potato plantlet free of viruses. Resumen La papa es un cultivo de importancia económica agro-industrial que se propaga de manera convencional, no obstante, la potencial transmisión de virus a través del tubérculo-semilla de generación en generación es una limitación mayor en la producción del rendimiento. Con el fin de producir papa libre de virus y suficiente cantidad de tubérculo-semilla de papa, se han desarrollado varias estrategias de métodos in vitro para la eliminación de los virus. El cultivo de meristemos se ha usado solo o combinado con técnicas tales como termoterapia, electroterapia, crioterapia y quimioterapia, como el mejor método alternativo para tratar papa infectada por virus. La literatura reciente ha demostrado que para eliminar virus significativamente se depende de la variedad de papa, agentes antivirales, tipo de virus, la duración del tratamiento térmico. Aun esta bajo investigación la duración apropiada para la eliminación eficiente. El nivel de la eliminación del virus puede detectarse por métodos serológicos, tales como el inmunoensayo con enzimas conjugadas (ELISA) y técnica de biología molecular, como la reverso-transcripción de reacción en cadena de la polimerasa en tiempo real (RT-PCR) que se usan para la detección del virus pre y post eliminación para evaluar la eficiencia y la precisión del método de eliminación del virus. El propósito de esta revisión es resaltar los métodos de eliminación de virus en papa y en la recomendación de la herramienta más efectiva para la detección de virus para asegurar la producción de plántulas de papa libres de virus.

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“…Karena cara kerja khemoterapi adalah menghambat terjadinya replikasi virus, menghambat transport virus antarsel dan antar jaringan tanaman. Beberapa jenis bahan kimia antiviral banyak dimanfaatkan dalam kultur in vitro adalah analog sintetik dari guanosin, yaitu ribavirin (Al Maari, et al, 2012), dithiouracyl (Loythala et al, 2008) dan saat ini yang banyak dipakai adalah acycloguanin atau acyclovir. Acyclovir berfungsi sebagai antimetabolite yang dapat bersifat sebagai inhihibitor DNA Polymerase dari virus.…”

Section: Pendahuluanunclassified

“…Terlihat bahwa terdapat perbedaan panjang akar yang sangat berbeda nyata pada pemberian konsentrasi aniviral acyclovir yang semakin tinggi. Menurut (Al Maari et al, 2012) bahwa konsentrasi antiviral yang semakin tinggi akan mengakibatkan pertumbuhan yang melemah sebagai akibat dari kematian meristem atau penurunan metabolisme seluler pada tanaman inang. Pengaruh Waktu pemaparan Hasil uji beda nyata jujur (uji Tukey) taraf 5% menunjukkan bahwa perlakuan waktu pemaparan berpengaruh terhadap panjang akar tanaman,jumlah tunas, dan tinggi tanaman semakin lama waktu pemaparan maka semakin tinggi nilai panjang akar tanaman, jumlah tunas, dan tinggi tanaman(Gambar 5, 6, 7).…”

Section: Hasil Dan Pembahasan Pengaruh Interaksi Konsentrasi Antiviraunclassified

The Effect of Concentration and Exposure Time Acyclovir for Elimination Sugarcane Mosaic Virus (SCMV) on The Apical Bud Culture of Sugarcane PS 881

Maisaro

Sugiharto²,

Dewanti

2017

J. I. Dasar

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SCMV (Sugarcane Mosaik Virus) is sugarcane crop disease resulting in chlorosis in leaves with the formation of the colors yellow and green intermittents. Based on survey information obtained that the air observation of all varieties of sugarcane was already stricken with the virus SCMV. Even the most formidable attack is on PS881 varieties with the intensity of the attacks reached 80%, so that it is estimated will lose up to 40% of the harvest. The sugarcane is virus free can be obtained via organogenesis in tissue culture method directly on the apical meristem, somatic embryogenesis at, and also with the addition of khemoterapeutan (acyclovir).The workings of the khemoterapi materials are the chemotherapi will interfere with replication and synthesis of genetic material of the virus but also cause the same effect against the mechanism of synthesis of nuklet acid on plants hosts. This research aims to find the best concentration and exposure time the most good in eliminating viruses SCMV in the apical bud culture of sugarcane PS881, using the antiviral acyclovir in conditions of invitro, so that the resulting plant will be virus free. The methods used to detect the presence of the virus by using the two ways, the first is by serology test through the protein content of the virus / checking nucleic acid virus with ELISA and the second is by RT-PCR. The results of the analysis showed that the interaction between the concentration of khemoterapi and exposure time produces the best treatment in eliminating the virus was . treatment with acyclovir concentration of 20 ppm and exposure time of 5 weeks. Keywords : ELISA, RT-PCR, sugarcane Mosaic virus, SCMV, PS 881 PENDAHULUANTanaman tebu merupakan bahan baku utama pembuatan gula. Gula merupakan komoditi penting bagi masyarakat Indonesia bahkan bagi masyarakat dunia. Kondisi geografis Indonesia sangat berpotensi untuk menghasilkan tanaman tebu sehingga menjadikan Indonesia sebagai negara yang berpotensi sebagai produsen gula terbesar di dunia. Namun pada tahun 2008 Indonesia mengimpor gula sebesar 2,3 juta ton setara raw sugar, yang terdiri dari gula putih, gula rafinasi dan gula mentah. Kebutuhan impor gula ini akan semakin tinggi seiring dengan banyaknya tanaman tebu yang terserang panyakit, sehingga dapat menurunkan produksi gula. Berdasarkan survei pengamatan lapang, mendapatkan informasi bahwa semua varietas tebu sudah terserang virus SCMV.SCMV adalah penyakit pada tanaman tebu dengan gejala yang sangat penting yaitu adanya garis-garis berwarna kuning kehijauan dengan latarbelakang warna hijau daun sehingga menyerupai gambaran mosaik pada daun, yang sangat tampak pada daun yang masih muda (Lilik & Tri 2009). Penyakit ini adalah penyakit yang sangat serius karena dapat menurunkan produksi gula 0,2% sampai dengan 50% tergantung tingkat infeksi virus dan ketahanan varietas terhadap penyakit mosaik (M. Andriani et al., 2012). Menurut observasi survei pengamatan lapang yang kami lakukan bersama petugas di perkebunan Jatiroto Jember, di dapatkan bahwa sekitar 60...

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Evaluation of some therapies and meristem culture to eliminate Potato Y potyvirus from infected potato plants

Abstract: ). Finally, the highest percentage virus free plantlets of PVY (93% in Binella and 87% in Burren) were obtained from meristem-tips 100 µm in length excised a er electric treatments (15 mA/10 min).

Cited by 28 publications

References 17 publications

In vitro thermotherapy-based methods for plant virus eradication

In vitro thermotherapy-based methods for plant virus eradication

Major In Vitro Techniques for Potato Virus Elimination and Post Eradication Detection Methods. A Review

The Effect of Concentration and Exposure Time Acyclovir for Elimination Sugarcane Mosaic Virus (SCMV) on The Apical Bud Culture of Sugarcane PS 881

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