Evaluation of sperm DNA fragmentation index, Zinc concentration and seminal parameters from infertile men with varicocele

Nguyen, Thi Trang; Trieu, Tien Sang; Tran, Thi Oanh; Luong, Thi Lan Anh

doi:10.1111/and.13184

Cited by 23 publications

(25 citation statements)

References 45 publications

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“…SDF testing can serve as an ancillary test to conventional semen analysis in specific clinical scenarios. Evidence indicates that higher levels of SDF are observed in patients who are unable to conceive naturally [149,[273][274][275], who present with UMI/idiopathic infertility [185,188,[276][277][278][279][280][281][282], have RPL [116,138,[180][181][182][183][283][284][285][286][287][288][289][290][291][292][293][294][295], are diagnosed with varicocele [169][170][171][172][173][174][296][297][298][299][300][301][302][303][304]…”

Section: Strengthsmentioning

confidence: 99%

“…SDF testing can serve as an ancillary test to conventional semen analysis in specific clinical scenarios. Evidence indicates that higher levels of SDF are observed in patients who are unable to conceive naturally [ 149 273 274 275 ], who present with UMI/idiopathic infertility [ 185 188 276 277 278 279 280 281 282 ], have RPL [ 116 138 180 181 182 183 283 284 285 286 287 288 289 290 291 292 293 294 295 ], are diagnosed with varicocele [ 169 170 171 172 173 174 296 297 298 299 300 301 302 303 304 305 ], have a negative ART outcome [ 13 41 88 117 306 307 308 309 310 311 312 313 314 315 316 317 318 319 320 321 322 323 324 325 326 327 …”

Section: Strengths-weaknesses-opportunities-threats (Swot) Analysis Omentioning

confidence: 99%

See 1 more Smart Citation

Sperm DNA Fragmentation: A New Guideline for Clinicians

Agarwal

Majzoub

Baskaran

et al. 2020

World J Mens Health

186

144

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Sperm DNA integrity is crucial for fertilization and development of healthy offspring. The spermatozoon undergoes extensive molecular remodeling of its nucleus during later phases of spermatogenesis, which imparts compaction and protects the genetic content. Testicular (defective maturation and abortive apoptosis) and post-testicular (oxidative stress) mechanisms are implicated in the etiology of sperm DNA fragmentation (SDF), which affects both natural and assisted reproduction. Several clinical and environmental factors are known to negatively impact sperm DNA integrity. An increasing number of reports emphasizes the direct relationship between sperm DNA damage and male infertility. Currently, several assays are available to assess sperm DNA damage, however, routine assessment of SDF in clinical practice is not recommended by professional organizations. This article provides an overview of SDF types, origin and comparative analysis of various SDF assays while primarily focusing on the clinical indications of SDF testing. Importantly, we report four clinical cases where SDF testing had played a significant role in improving fertility outcome. In light of these clinical case reports and recent scientific evidence, this review provides expert recommendations on SDF testing and examines the advantages and drawbacks of the clinical utility of SDF testing using Strength-Weaknesses-Opportunities-Threats (SWOT) analysis.

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Section: Strengthsmentioning

confidence: 99%

“…SDF testing can serve as an ancillary test to conventional semen analysis in specific clinical scenarios. Evidence indicates that higher levels of SDF are observed in patients who are unable to conceive naturally [ 149 273 274 275 ], who present with UMI/idiopathic infertility [ 185 188 276 277 278 279 280 281 282 ], have RPL [ 116 138 180 181 182 183 283 284 285 286 287 288 289 290 291 292 293 294 295 ], are diagnosed with varicocele [ 169 170 171 172 173 174 296 297 298 299 300 301 302 303 304 305 ], have a negative ART outcome [ 13 41 88 117 306 307 308 309 310 311 312 313 314 315 316 317 318 319 320 321 322 323 324 325 326 327 …”

Section: Strengths-weaknesses-opportunities-threats (Swot) Analysis Omentioning

confidence: 99%

Sperm DNA Fragmentation: A New Guideline for Clinicians

Agarwal

Majzoub

Baskaran

et al. 2020

World J Mens Health

186

144

View full text Add to dashboard Cite

show abstract

“…Zinc also plays an important role in testicular development and sperm formation [3]. Zinc deficiency significantly enhances apoptosis of germ cells in mouse testis and causes spermatogenesis arrest and fertilization damage [4]. Studies have shown that obese men are 3.5 times more likely to have oligozoospermia than men with normal weight [5,6].…”

Section: Introductionmentioning

confidence: 99%

The Mechanism of Zinc Sulfate in Improving Fertility in Obese Rats Analyzed by Sperm Proteomic Analysis

Ma¹,

Han²,

et al. 2020

BioMed Research International

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This study investigates the mechanism underlying the improving effect of zinc on fertility in obese rats using proteomics. The effects of three different doses of ZnSO4 on spermatogenesis and hormone levels were studied. Testicular spermatogenesis was observed by HE staining. Serum estrogen and testosterone levels were measured by chemiluminescent microparticle immunoassay. Sperm proteomic analysis was performed by liquid chromatography-mass spectrometry. The DAVID database was used to perform the GO enrichment analysis and KEGG pathway analysis of the differentially expressed genes, and the STRING online database was used to construct a PPI network. The sperm count, sperm motility, and testosterone hormones of the ZnSO4-treated rats group were increased. ZnSO4 improved testicular structure and spermatogenesis abnormalities caused by obesity. Proteomic analysis showed that there were 401 differentially expressed proteins in a total of 6 sperm samples from the ZnSO4-treated group and the obesity groups. Differential proteins were input into the DAVID website. The 341 identified proteins were then classified according to their biological functions. The KEGG analysis showed that the enriched signal pathways included glycolysis/gluconeogenesis, carbon metabolism, citrate cycle, fatty acid metabolism, and pyruvate metabolism. Some proteins were shown to be associated with valine, leucine, and isoleucine degradation pathways. STRING analysis obtained 36 node proteins. Cytoscape analysis showed that these proteins mainly participated in nine networks including metabolic process, oxidation-reduction, aerobic respiration, RNA splicing, and glutathione conjugation. ZnSO4 may improve the fertility of obese male rats by regulating protein expression related to metabolism, inflammation, and sperm maturation.

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“…In a prospective study, the semen quality of 179 infertile men with history of at least one-year VCL was examined. The findings recorded an inverse correlation between zinc concentration and DNA fragmentation degrees of spermatozoa (Nguyen et al, 2019).…”

Section: Zincmentioning

confidence: 83%

Dietary supplements in the management of varicocele‐induced infertility: A review of potential mechanisms

2020

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Varicocele is a main cause of lower production of spermatozoon and infertility with multiple pathophysiological mechanisms. In the past decades, the use of dietary supplements has significantly increased due to both the modern lifestyle and the food shortages of the industrialised countries. The purpose of this review paper is to collect scientific evidences from basic and clinical studies which support the use of dietary supplements to define the clinical framework for patients with varicocele. In the present review, we used keywords such as dietary supplements, varicocele, male infertility, oxidative stress, DNA fragmentation, sperm parameters to find the proper articles. The standard search biomedical engines were used for seeking the papers. The use of dietary supplements such as minerals, vitamins and antioxidants has an essential role in the prevention and treatment of varicocele by increasing the levels of antioxidant enzymes (e.g. peroxidase, superoxide dismutase and catalase) and decreasing the levels of inflammatory markers (e.g. tumour necrosis factor-α, interleukin-6 and interleukin-1) in testis. According to the results, the dietary supplements may alleviate the spermatogenesis in varicocele patients through different mechanisms such as suppression of stress oxidative and inflammation in testicular tissue.

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Evaluation of sperm DNA fragmentation index, Zinc concentration and seminal parameters from infertile men with varicocele

Cited by 23 publications

References 45 publications

Sperm DNA Fragmentation: A New Guideline for Clinicians

Sperm DNA Fragmentation: A New Guideline for Clinicians

The Mechanism of Zinc Sulfate in Improving Fertility in Obese Rats Analyzed by Sperm Proteomic Analysis

Dietary supplements in the management of varicocele‐induced infertility: A review of potential mechanisms

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