Evaluation of sperm head dimensions and chromatin integrity of epididymal sperm from domestic cats using the toluidine blue technique

Alves, Izabella Pazzoto; Cancelli, C. H. B.; Grassi, Thiago Luís Magnani; Oliveira, Patricia Ramos Heggendorn; Franciscato, Douglas Augusto; Carreira, Janaina Torres; Koivisto, Marion Burkhardt de

doi:10.1016/j.anireprosci.2018.08.001

Cited by 12 publications

(7 citation statements)

References 36 publications

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“…The sperm abnormalities found in the present study included doubleheader with the coiled tail, bent neck, double tail, macrocephalic with double tail, microcephalic, elongated head, and hairpin tail. The sperm abnormalities in this study were higher when compared with the normal value reported by Alves et al (2018). Alves et al (2018) reported that the normal value of cat sperm abnormalities was about 16.7 ± 9.9%.…”

Section: Discussioncontrasting

confidence: 67%

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Sperm Quality and Histology of the Testis and Epididymis in Chemical Castrated Male Cats with Intra-testicular Injection of Sodium Chloride Solution

Dayanti¹,

Berata²,

Puja³

2021

WVJ

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The high population of feral cats in Bali, Indonesia, could be a problem associated with cat welfare and the risk of zoonotic diseases. Gonadectomy or male castration is an option for population control; however, there would be more economical ways to reach this goal. Sterilization using chemicals is an alternative to the surgical method of castration as it is reasonably priced, activated in a short time, and does not affect animal activity after the chemical administration. The present study aimed to evaluate intratesticular injection of Sodium chloride as an agent of chemosterilants in male cats. In the current study, 16 healthy male cats aged nearly one year old were randomly selected. The male cats were allocated into four groups containing four cats in each. Group 1 (control) received bilateral intratesticular injection with 0.25 mL normal saline solution containing 2% Lidocaine. The cats in groups 2, 3, and 4 received bilateral intratesticular injection of 0.25 mL Sodium chloride solution containing 2% Lidocaine with a concentration of 15%, 20%, and 25%, respectively. At the end of the study (after 30 days of injection), the cats were castrated and their testes were removed for sperm quality and histopathological evaluation. The results of the present study indicated that intratesticular injection of Sodium chloride significantly decreased the motility and viability rates, and increased the abnormalities of the sperms. Histopathological studies revealed marked depletion of spermatozoa in the testes and seminiferous tubular degeneration, seminiferous tubular atrophy, and epithelial cell vacuolation. In conclusion, the injection of 20-25% solution of Sodium chloride in testes had the potential as a chemosterilant for chemical sterilization in cats.

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Section: Discussioncontrasting

confidence: 67%

“…The sperm abnormalities in this study were higher when compared with the normal value reported by Alves et al (2018). Alves et al (2018) reported that the normal value of cat sperm abnormalities was about 16.7 ± 9.9%.…”

Section: Discussioncontrasting

confidence: 67%

Sperm Quality and Histology of the Testis and Epididymis in Chemical Castrated Male Cats with Intra-testicular Injection of Sodium Chloride Solution

Dayanti¹,

Berata²,

Puja³

2021

WVJ

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“…Two hundred spermatozoa per smear were examined under a light microscope by two embryologists at ×100 magnification. The cells were classified into two groups: TB positive cells (TB+) (dark violet cells; altered chromatin) and TB negative cells (TB−) (light blue cells; normal chromatin structure) (Tsarev et al ., 2009; Alves et al ., 2018).…”

Section: Methodsmentioning

confidence: 99%

What are the effects of vitamin C on sperm functional properties during direct swim-up procedure?

Raad¹,

Mansour²,

Ibrahim³

et al. 2019

Zygote

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SummaryDirect swim-up procedure is widely used to separate the motile competent spermatozoa from the antioxidant-rich semen. Subsequently, spermatozoa become more vulnerable to reactive oxygen species (ROS) due to their cytological characteristics. The effect of vitamin C, a highly concentrated antioxidant in the semen, on direct swim-up-enriched sperm population is not fully investigated. Therefore, the aim of the present study was to assess the effect of vitamin C on sperm functional properties during direct swim-up procedure. Semen samples were collected from 22 participants. Each semen sample was divided into several aliquots. The first portion was overlaid with sperm medium without ascorbic acid (0 µM AA). The second and third fractions were overlaid with sperm medium supplemented with 300 µM and 600 µM AA; respectively. After 1 h of incubation, basic sperm parameters, intracellular ROS levels, acrosome reaction, chromatin integrity, and glucose uptake were assessed. Swim-up without AA significantly increased the percentage of ROS(+) spermatozoa compared with the raw semen (P<0.01). Interestingly, swim-up with 300 µM AA did not increase the percentage of ROS(+) sperm compared with the raw semen. In parallel, the percentage of sperm with altered chromatin integrity was significantly lower in the 300 µM AA group compared with that in the raw semen (P<0.05). These findings suggest that supplementation of vitamin C to sperm medium could be beneficial for direct swim-up-derived spermatozoa.

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“…In a study conducted on sperm collected from feline epididymides, smaller dimensions of sperm heads in the epididymal cauda were found than in the caput and corpus [23]. The study of these authors also found that the sperm head dimension was correlated with chromatin decondensation, and the sperm head size decreased as the sperm were trans-ported through the three epididymal regions.…”

Section: Discussionmentioning

confidence: 94%

Evaluation of the Morphometry of Sperm from the Epididymides of Dogs Using Different Staining Methods

Wysokińska

Wójcik

Chłopik

2021

Animals

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Evaluation of sperm morphometry is an important criterion in the diagnosis of a male animal’s suitability for breeding. The aim of the study was to evaluate the morphometry of sperm from the epididymides of dogs subjected to routine castration using various staining methods. The study was carried out on semen collected from ten healthy dogs. Gonads were obtained from each dog during routine castration at a veterinary surgery. Then, the epididymides (caput, corpus, cauda) were isolated from the gonads, semen was collected from them and microscope slides were prepared. The slides for evaluation of sperm morphometry were prepared by four methods: DiffQuik, SpermBlue, eosin-nigrosin and eosin-gentian. A total of 2400 sperm were analyzed (240 sperm from the dog). The sperm collected from the caput and corpus of the epididymis were found to have larger heads and tails than those collected from the cauda of the epididymis. The staining method was shown to affect the morphometry of sperm taken from the epididymides of dogs. The staining methods differentiate the dimensions of the head of sperm in different parts of the epididymis but do not affect the length of the sperm tail. The occurrence of differences in the head dimensions of sperm may be linked to the use of different fixatives and chemical reagents in the staining procedure. Sperm stained by the EN method had the smallest head and tail dimensions. The greatest head area was noted in the sperm stained by the EG method. In the slides stained by the SB method, the sperm heads were relatively long but narrow. The methods used are suitable for the evaluation of sperm structure, and the possibility of using all four methods enables a full characterization of sperm collected from the caput, corpus and cauda of the epididymides of dogs.

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Evaluation of sperm head dimensions and chromatin integrity of epididymal sperm from domestic cats using the toluidine blue technique

Cited by 12 publications

References 36 publications

Sperm Quality and Histology of the Testis and Epididymis in Chemical Castrated Male Cats with Intra-testicular Injection of Sodium Chloride Solution

Sperm Quality and Histology of the Testis and Epididymis in Chemical Castrated Male Cats with Intra-testicular Injection of Sodium Chloride Solution

What are the effects of vitamin C on sperm functional properties during direct swim-up procedure?

Evaluation of the Morphometry of Sperm from the Epididymides of Dogs Using Different Staining Methods

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