What are the effects of vitamin C on sperm functional properties during direct swim-up procedure?

Raad, Georges; Mansour, Joyce; Ibrahim, Rim; Azoury, Jessica; Azoury, J.; Mourad, Youmna; Fakih, Chadi; Azoury, Joseph

doi:10.1017/s0967199419000030

Cited by 16 publications

(24 citation statements)

References 57 publications

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“…This process can lead to damages to polyunsaturated lipids present in sperm plasma membrane, as well as to the DNA, RNA and proteins (Atig, Kerkeni, Saad, & Ajina, ; Kamkar, Ramezanali, & Sabbaghian, ; Mello Filho et al, ; Sposito et al, ). This imbalance can also be observed in reproductive biotechnologies, (e.g., semen cryopreservation; Amidi, Pazhohan, Shabani, Khodarahmian, & Nekoonam, ; Panner Selvam et al, ), in vitro fertilisation and in protocols with an excessive manipulation of semen, as plasma separation through centrifugation (Donnelly et al, ) and also sperm separation (Raad et al, ). However ROS, under physiological conditions, are important to many process can occur, such as events involving reproductive process, like sperm capacitation, hypermotility, cellular activation, interaction with zona pellucida and acrosome reaction (Banihani et al, ; Moretti et al, ; Satorre et al, ).…”

Section: Discussionmentioning

confidence: 99%

“…Most reports describe that antioxidant supplementation in semen may improve the oxidative equilibrium (Bousnane, May, Yahia, & Abu‐Alhaija, ). This has been attempted during cryopreservation (Panner Selvam, Henkel, Sharma, & Agarwal, ; Satorre, Breininger, & Beconi, ), in vitro fertilisation (Griveau & Le Lannou, ) and semen processing (Hughes, Lewis, McKelvey‐Martin, & Thompson, ; Raad et al, ), to name a few. Moreover, semen processing by density gradient centrifugation, which is commonly used in assisted reproduction technologies (Harlev, Henkel, Samanta, & Agarwal, ; Inoue et al, ; Walters et al, ), has a demonstrated capacity to lead to sperm oxidative damage (Aitken & Clarkson, ; Henkel & Schill, ).…”

Section: Introductionmentioning

confidence: 99%

“…However some findings are inconclusive. Some studies demonstrating positive outcomes from sample antioxidant supplementation (Adami et al, ; Aitken, Clarkson, & Fishel, ; Burton & Ingold, ; Hughes et al, ; Ribas‐Maynou et al, ), others demonstrating no effect (Raad et al, ; Sigman, Glass, Campagnone, & Pryor, ) and others still a negative effect (Donnelly et al, ).…”

Section: Introductionmentioning

confidence: 99%

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Carnosine treatment during human semen processing by discontinuous density gradient

et al. 2019

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The aim of this article was to evaluate the effects of different concentrations of carnosine added during human semen processing. Semen samples from 34 patients were submitted to processing by discontinuous density gradient centrifugation without (control) or with different concentrations of carnosine supplementation as follows: (a) 20 mM of carnosine supplementation on the layers of Percoll; and (b) 50 mM carnosine supplementation. Sperm samples were then washed with human tubal fluid medium and evaluated according to sperm kinetics and functional assessment. For statistical analysis, data were evaluated by a general linear model or a Friedman test, whenever appropriate. The 50 mM carnosine supplementation led to improved sperm mitochondrial activity when compared to untreated samples. Motility variables, such as percentage of motile and progressively motile spermatozoa, average path velocity, straight line velocity, curvilinear velocity and linearity, showed an improvement after semen processing irrespective of carnosine supplementation. Both concentrations of carnosine increased the beat‐cross frequency (BCF) when compared to samples before processing. We conclude that carnosine supplementation in semen samples benefits sperm mitochondrial activity and BCF.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Carnosine treatment during human semen processing by discontinuous density gradient

et al. 2019

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“…In particular vitamin C and vitamin E are of interest because the body cannot synthesize them and therefore systemic concentrations directly relate to dietary intake [59,60]. Previous increased dietary intake of vitamin C in men reduced sperm oxidative DNA damage (8OHdG) [61], while in vitro exposure of sperm to 300 µM of ascorbic acid (the active component of vitamin C) had beneficial effects on sperm intracellular ROS levels and sperm chromatin integrity [62]. The same has also been shown for vitamin E both in vitro for protecting human sperm from induced oxidative stress at 40 μmol 1-1 vitamin E [63], and through oral supplementation of sub-fertile men to reduce sperm DNA fragmentation and oxidative stress [37].…”

Section: Discussionmentioning

confidence: 99%

Dietary Micronutrient Supplementation for 12 Days in Obese Male Mice Restores Sperm Oxidative Stress

et al. 2019

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Male obesity, which often co-presents with micronutrient deficiencies, is associated with sub-fertility. Here we investigate whether short-term dietary supplementation of micronutrients (zinc, selenium, lycopene, vitamins E and C, folic acid, and green tea extract) to obese mice for 12 days (designed to span the epididymal transit) could improve sperm quality and fetal outcomes. Five-week-old C57BL6 males were fed a control diet (CD, n = 24) or high fat diet (HFD, n = 24) for 10 weeks before allocation to the 12-day intervention of maintaining their original diets (CD, n = 12, HFD n = 12) or with micronutrient supplementation (CD + S, n = 12, HFD + S, n = 12). Measures of sperm quality (motility, morphology, capacitation, binding), sperm oxidative stress (DCFDA, MSR, and 8OHdG), early embryo development (2-cell cleavage, 8OHdG), and fetal outcomes were assessed. HFD + S males had reduced sperm intracellular reactive oxygen species (ROS) concentrations and 8OHdG lesions, which resulted in reduced 8OHdG lesions in the male pronucleus, increased 2-cell cleavage rates, and partial restoration of fetal weight similar to controls. Sub-fertility associated with male obesity may be restored with very short-term micronutrient supplementation that targets the timing of the transit of sperm through the epididymis, which is the developmental window where sperm are the most susceptible to oxidative damage.

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“…The average age of first-time fathers is increasing worldwide. In the past 60 years in Australia, the median age of fathers has increased from 29.0 years to now 33.1 years (~15%) [1], with 10% of all naturally conceived births now coming from fathers ≥40 years [2]. These statistics are heightened within couples seeking assisted reproductive technology (ART) treatment, due to the strong relationship between paternal age and sub-fertility [3][4][5][6].…”

Section: Introductionmentioning

confidence: 99%

Improving Sperm Oxidative Stress and Embryo Quality in Advanced Paternal Age Using Idebenone In Vitro—A Proof-of-Concept Study

2021

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Advanced paternal age is associated with increased sperm reactive oxygen species (ROS) and decreased fertilization and pregnancy rates. Sperm washing during infertility treatment provides an opportunity to reduce high sperm ROS concentrations associated with advanced paternal age through the addition of idebenone. Sperm from men aged >40 years and older CBAF1 mice (12–18 months), were treated with 5 µM and 50 µM of idebenone and intracellular and superoxide ROS concentrations assessed. Following in vitro fertilization (IVF), embryo development, blastocyst differentiation, DNA damage and cryosurvival, pregnancy and implantation rates and fetal and placental weights were assessed. Five µM of idebenone given to aged human and mouse sperm reduced superoxide concentrations ~20% (p < 0.05), while both 5 and 50 µM reduced sperm intracellular ROS concentrations in mice ~30% (p < 0.05). Following IVF, 5 µM of idebenone to aged sperm increased fertilization rates (65% vs. 60%, p < 0.05), blastocyst total, trophectoderm and inner cell mass cell numbers (73 vs. 66, 53 vs. 47 and 27 vs. 24, respectively, p < 0.01). Treatment with idebenone also increased blastocyst cryosurvival rates (96% vs. 78%, p < 0.01) and implantation rates following embryo transfer (35% vs. 18%, p < 0.01). Placental weights were smaller (107 mg vs. 138 mg, p < 0.05), resulting in a larger fetal to placental weight ratio (8.3 vs. 6.3, p = 0.07) after sperm idebenone treatment. Increased sperm ROS concentrations associated with advanced paternal age are reduced with the addition of idebenone in vitro, and are associated with improved fertilization rates, embryo quality and implantation rates after IVF.

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What are the effects of vitamin C on sperm functional properties during direct swim-up procedure?

Cited by 16 publications

References 57 publications

Carnosine treatment during human semen processing by discontinuous density gradient

Carnosine treatment during human semen processing by discontinuous density gradient

Dietary Micronutrient Supplementation for 12 Days in Obese Male Mice Restores Sperm Oxidative Stress

Improving Sperm Oxidative Stress and Embryo Quality in Advanced Paternal Age Using Idebenone In Vitro—A Proof-of-Concept Study

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