Evaluation of spermatogenic activity of Annona muricata methanolic bark extract in Albino rats

Abstract

Annona muricata is employed in the treatments of variety of diseases ranging from arthritic pains, investigations have substantiated its use as hypoglycemic, anticancer and antidiabetic agent [6].Despite its medicinal uses, the plant has a very important compound called Annonacin which B ackground: Annona muricata has been widely used by majority of people in the treatment of diabetes, pain, fever, cancer and many others diseases. Despite these medicinal values of this plant, it is important to study its effect on semen factors that determines reproductive health. Materials and method:Sixteen male rats weighing 150-200g were given Annona muricata methanolic bark extract (AMMBE) for fourteen days at the dose rate of 0 mg kg -1 , 200 mgkg -1 , 400 mgkg -1 and 800 mgkg -1 representing groups A, B, C and D respectively. The sperm cells were analyzed to determine the sperm counts, morphological appearances and survivability. Testes were also fixed in formalin for histopathology. The results obtained were analyzed using standard statistical package. Results:The results showed that the sperm liveability of rats treated with Annona muricata methanolic bark extract (AMMBE) were statistically significantly lower in groups B, C and D having mean values of 78.50±1.62, 86.50±3.11 and 68.00±1.96 respectively while the control group had 91.00±2.86, sperm motility was found to be highest in control group with 93.50±3.22 while other groups (B, C and D) had 82.25±2.20, 87.50±1.96 and 71.25±2.34 respectively. This finding showed strong correlation between the sperm motility and live-death ratio. In addition, there was reduction in the sperm concentration in a dose dependent manner, while the testicular histology revealed lesions that indicate potential for structural damages.Conclusion: It was concluded that due to the reductions in sperm motility, sperm concentration and live-death ratio, Annona muricata prolonged use may be detrimental.

The impact of Annona muricata and metformin on semen quality and hormonal profile in Arsenic trioxide-induced testicular dysfunction in male Wistar rat

“…The mechanism of action linked to ELAM improved sperm count is flavonoids and saponins, which tend to repair tissue proteins and combat ROS formation in the testicular plasma membrane. The study has accordance with the reports of the study (65,66,68,78,79), revealing improved sperm count in testicular insult following Annona muricata extract, but contradict the findings of Oladipo et al (80) indicating significantly lowered levels of total sperm count following Annona muricata bark extracts. However, MET treatment significantly improved total sperm count, which is linked to the decline in oxidative stress and lipid peroxidation, enhancement of 5'-AMP-activated protein kinase activity, and restoration to the expected levels of pituitary-gonadal hormones (28).…”