Evaluation of Spoligotyping and MIRU-VNTR for Mycobacterium bovis in Xinjiang, China

Sun, Zhonghe; Cao, Rui; Tian, Miao; Zhang, Xuxia; Zhang, Xiyue; Li, Yan; Xu, Yuhui; Fan, Weixing; Huang, Biao; Li, Chuanyou

doi:10.1016/j.rvsc.2011.03.012

Cited by 13 publications

(17 citation statements)

References 25 publications

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“…Interestingly, the genotype L has the same copy numbers in 7 MIRUs (MIRUs 2,10,23,27,31,39,and 40) and 2 ETRs (ETRs A and C) as the main genotype reported previously in China (Sun et al, 2012). However, these markers were similar to those reported in Chad and Belgium thus indicating that such patterns may not be specific to M. bovis isolates in Asia (Hilty et al, 2005;Allix et al, 2006).…”

Section: Discussionsupporting

confidence: 48%

“…Of 131 M. bovis isolates, 91 (69.5%) have 2-4-4-3 copies and 40 (30.5%) have 3-3-5-2 copies of MIRU 31, QUB 26, VNTR 2401, and VNTR 3171, respectively. Interestingly, these 4 VNTR markers have no linkage with other polymorphic VNTR loci, and studies in other countries did not show such marker linkage Supply et al, 2006;Martinez et al, 2008;Sun et al, 2012). According to the linkage of 4 markers, the dendrogram or MST analysis showed the isolates are classified into 2 different groups and 2 genotypes (K and L) occupy most of each group.…”

Section: Discussionmentioning

confidence: 90%

“…For molecular epidemiology of M. bovis, therefore, we recommend VNTRs including the 15 loci in this study, which are also common in the previous studies from other regions. These loci can be thus useful to share of data for the international research (Hilty et al, 2005;Allix et al, 2006;Sun et al, 2012). Furthermore, because recently developed VNTR markers are more hypervariable than previous MIRUs and ETRs, new VNTR loci can be identified and applicable for closer analysis of M. bovis transmission among animal populations.…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Extent of Mycobacterium bovis transmission among animals of dairy and beef cattle and deer farms in South Korea determined by variable-number tandem repeats typing

Jeon

et al. 2015

Veterinary Microbiology

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Section: Discussionsupporting

confidence: 48%

Section: Discussionmentioning

confidence: 90%

Section: Discussionmentioning

confidence: 99%

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Extent of Mycobacterium bovis transmission among animals of dairy and beef cattle and deer farms in South Korea determined by variable-number tandem repeats typing

Jeon

et al. 2015

Veterinary Microbiology

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“…Genetic diversity for a locus is calculated as h = 1 – Σ x i 2 [ n /( n – 1)], where x i is the frequency of the i th allele at the locus, and n is the number of isolates [18]. …”

Section: Methodsmentioning

confidence: 99%

“…This entails fundamental methods that could initially prohibit the generation of infectious particles and then prevent their spread and reduce or eliminate them. The promptitude of diagnosis, isolation, and treatment of people with active tuberculosis is an effective control program [2, 3]. …”

Section: Introductionmentioning

confidence: 99%

Determining the risk of intra-community transmission of tuberculosis in the northwest of Iran through 15 loci MIRU-VNTR typing

Afaghi-Gharamaleki

Moaddab

Darbouy

et al. 2017

EuJMI

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This study was carried out in order to investigating the effect of travelling on the transmission of tuberculosis from high- to low-burden TB countries. Mycobacteria samples isolated from patients of distinct and relatively co-related countries (Azerbaijan Republic and Tabriz [located in the northwest of Iran]) were analyzed through 15 loci MIRU-VNTR typing method. PCR was done using special primers for each of the loci; then the number of allele repeats for all loci were determined by the size of their fragments. Finally, the created numeric patterns for each isolate were analyzed and clustered, using MIRU-VNTRplus.org website. All 119 isolates dispersing at 106 distinct patterns were composed of 10 clusters with 23 members and 96 unique patterns. Nine and five loci had high and moderate discriminatory power, respectively, but only one of them was poor in clustering. The study showed that 89.08% of TB cases involved resulted from the reactivation pattern and 10.92% were related to ongoing transmission. Although Azerbaijan Republic is a higher-burden TB region than Tabriz and Azerbaijan people make frequent tours to Tabriz to receive low or free medical services, the findings showed no TB transmission from the regions at least during the year of the study.

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Isolation and molecular characterization of Mycobacterium bovis from Kafue lechwe (Kobus leche kafuensis) from Zambia

Malama

Johansen

Muma

et al. 2013

Trop Anim Health Prod

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Bovine tuberculosis (BTB) is a chronic bacterial disease caused by Mycobacterium bovis. Infections due to M. bovis, which serves as a stable reservoir, can pose serious challenge to control and eradicate in both wildlife and livestock at the interface. This study aimed at isolating and characterizing M. bovis from Kafue lechwe (Kobus leche kafuensis) and black lechwe (Kobus leche smithemani) at the animal/human interface in Zambia. The samples with lesions compatible with BTB collected during the hunting seasons of 2009 and 2010 were cultured for isolation of mycobacteria using Stonebrink with pyruvate (BD Diagnostics, MD, USA) and Middlebrook 7H10 (BD Diagnostics) slants. Isolated mycobacteria were identified using IS6110 polymerase chain reaction and deletion analysis. Molecular characterization of the isolates was performed using spoligotyping and mycobacteria interspersed repetitive unit-variable number tandem repeat (MIRU-VNTR) with nine loci. Data was analyzed using BioNumerics software 6.1. Out of the 39 samples, acid fast bacilli were detected in 27 (69.2 %) based on smear microscopy. Seven isolates were found to belong to Mycobacterium tuberculosis complex, and all were identified as M. bovis based on deletion analysis. All seven isolates were identical on spoligotyping as belonging to the SB0120 (SIT 482). MIRU-VNTR differentiated the isolates into five different patterns. This study has confirmed that M. bovis circulates in the Kafue lechwe, and non-tuberculous mycobacteria were detected in the black lechwe in Zambia which represents a wildlife reservoir, with a potential to spillover to cattle and humans. Isolates of M. bovis from lechwe antelopes are much conserved as only one spoligotype was detected. The study has shown that three loci differentiated fairly well. This option is cheap and less laborious, and hence a better option in resource-strained country like Zambia. The study further showed that some of the loci recommended by the European Reference Laboratory are not suitable for typing M. bovis in Zambia.

show abstract

Evaluation of Spoligotyping and MIRU-VNTR for Mycobacterium bovis in Xinjiang, China

Cited by 13 publications

References 25 publications

Extent of Mycobacterium bovis transmission among animals of dairy and beef cattle and deer farms in South Korea determined by variable-number tandem repeats typing

Extent of Mycobacterium bovis transmission among animals of dairy and beef cattle and deer farms in South Korea determined by variable-number tandem repeats typing

Determining the risk of intra-community transmission of tuberculosis in the northwest of Iran through 15 loci MIRU-VNTR typing

Isolation and molecular characterization of Mycobacterium bovis from Kafue lechwe (Kobus leche kafuensis) from Zambia

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