Evaluation of stationary phases based on silica hydride for the analysis of drugs of abuse

Pesek, Joseph J.; Matyska, Maria T.; Kim, Amy

doi:10.1002/jssc.201300377

Cited by 20 publications

(16 citation statements)

References 20 publications

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“…Another type of bonded stationary phase that contains phenyl ligand is a phenyl‐hydride material . This type of stationary phase may be applied in both RP and aqueous normal‐phase chromatography.…”

Section: Introductionmentioning

confidence: 99%

Phenyl‐bonded stationary phases—The influence of polar functional groups on retention and selectivity in reversed‐phase liquid chromatography

Bocian

Buszewski

2014

J of Separation Science

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The chromatographic properties of four phenyl-bonded phases with different structures were studied. The columns used were packed with a stationary phase containing a phenyl ring attached to the silica surface using different types of linkage molecules. As a basic characteristic of the bonded phases, the hydrophobicity and silanol activity (polarity) were investigated. The presence of the polar amino and amide groups in the structure of the bonded ligand strongly influences the polarity of the bonded phase. Columns were compared according to methylene selectivity using a series of benzene homologues and according to their shape and size selectivity using polycyclic aromatic hydrocarbons. The measurements were done using methanol/water and acetonitrile/water mobile phases. The presented results show that the presence of polar functional groups in the ligand structure strongly influences the chromatographic properties of the bonded phase.

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Section: Introductionmentioning

confidence: 99%

Phenyl‐bonded stationary phases—The influence of polar functional groups on retention and selectivity in reversed‐phase liquid chromatography

Bocian

Buszewski

2014

J of Separation Science

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“…49 When this review was written, core-shell stationary phases were available just as type B silica materials, while ZirChrom PDB, which is a polybutadiene-coated zirconia stationary phase, was the only sub-2 mm non-silica stationary phase available in the market. 50 The type C silica [51][52][53][54][55][56] is a very promising material, and it is possible to prepare sub-2 mm totally porous and coreshell stationary phases based on type C silica. However, coreshells and sub-2 mm stationary phases based on Type C silica were not available, which may be attributed to the fact that type C silica has a small market.…”

Section: Sub-2 MM Stationary Phasesmentioning

confidence: 99%

Sub-2 μm fully porous and partially porous (core–shell) stationary phases for reversed phase liquid chromatography

2014

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“…Salt buffers are often critical additives to HPLC mobile phases in any separation mode, but are potentially detrimental to CAD performance. In HILIC, salt buffers can lead to better peak shape than simple acid solutions [13][14][15], thus we wished to investigate their influence on CAD sensitivity. Furthermore, as with other aerosol-based detectors, detector response is dependent on organic solvent content.…”

Section: Introductionmentioning

confidence: 99%

Performance of charged aerosol detection with hydrophilic interaction chromatography

Russell

Heaton

Underwood

et al. 2015

Journal of Chromatography A

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The performance of the charged aerosol detector (CAD) was investigated using a diverse set of 29 solutes, including acids, bases and neutrals, over a range of mobile phase compositions, particularly with regard to its suitability for use in hydrophilic interaction chromatography (HILIC). Flow injection analysis was employed as a rapid method to study detector performance. CAD response was 'quasi-universal', strong signals were observed for compounds that have low volatility at typical operating (room) temperature. For relatively involatile solutes, response was reasonably independent of solute chemistry, giving variation of 12-18% RSD from buffered 95% ACN (HILIC) to 10% ACN (RP). Somewhat higher response was obtained for basic compared with neutral solutes. For cationic basic solutes, use of anionic reagents of increasing size in the mobile phase (formic, trifluoroacetic and heptafluorobutyric acid) produced somewhat increased detector response, suggesting that salt formation with these reagents is contributory. However, the increase was not stoichiometric, pointing to a complex mechanism. In general, CAD response increased as the concentration of acetonitrile in the mobile phase was increased from highly aqueous (10% ACN) to values typical in the HILIC range (80-95% ACN), with signal to noise ratios about four times higher than those for the RP range. The response of the CAD is non-linear. Equations describing aerosol formation cannot entirely explain the shape of the plots. Limits of detection (determined with a column for solutes of low k) under HILIC conditions were of the order of 1-3ng on column, which compares favourably with other universal detectors. CAD response to inorganic anions allows observation of the independent movement through the column of the cationic and anionic constituents of basic drugs, which appear to be accompanied by mobile phase counterions, even at quite high solute concentrations.

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Evaluation of stationary phases based on silica hydride for the analysis of drugs of abuse

Cited by 20 publications

References 20 publications

Phenyl‐bonded stationary phases—The influence of polar functional groups on retention and selectivity in reversed‐phase liquid chromatography

Phenyl‐bonded stationary phases—The influence of polar functional groups on retention and selectivity in reversed‐phase liquid chromatography

Sub-2 μm fully porous and partially porous (core–shell) stationary phases for reversed phase liquid chromatography

Performance of charged aerosol detection with hydrophilic interaction chromatography

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