Evaluation of sterilisation methods for bio-ink components: gelatin, gelatin methacryloyl, hyaluronic acid and hyaluronic acid methacryloyl

O’Connell, Cathal; Onofrillo, Carmine; Duchi, Serena; Li, Xin; Zhang, Yifan; Tian, Peilin; Lu, Lanyita; Trengove, Anna; Quigley, Anita; Gambhir, Sanjeev; Khansari, Afsaneh; Mladenovska, Tajanka; O’Connor, Andrea J.; Bella, Claudia Di; Choong, Peter; Wallace, Gordon G.

doi:10.1088/1758-5090/ab0b7c

Cited by 55 publications

(77 citation statements)

References 79 publications

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“…[ 24 ] An increase in gelatin concentration has been shown to enhance the storage moduli of hydrogels, [ 32 ] and the addition of unmodified gelatin can act as a thickening agent to increase printability at room temperature. [ 33 ] Thus, gelatin was added to alginate‐GelMA bioink at 2.5%–5% w/v. The preliminary printability was assessed by evaluating the ability to form continuous filament and by observing filament morphology.…”

Section: Resultsmentioning

confidence: 99%

Encapsulation of Human Natural and Induced Regulatory T‐Cells in IL‐2 and CCL1 Supplemented Alginate‐GelMA Hydrogel for 3D Bioprinting

Kim

Hope

Gantumur

et al. 2020

Adv Funct Materials

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Regulatory T-cells (Tregs) are important modulators of the immune system through their intrinsic suppressive functions. Systemic adoptive transfer of ex vivo expanded Tregs has been extensively investigated for allogeneic transplantation. Due to the time-consuming and costly expansion protocols of Tregs, more targeted approaches could be beneficial. The encapsulation of human natural and induced Tregs for localized immunosuppression is described for the first time. Tregs encapsulated in alginate-gelatin methacryloyl hydrogel remain viable, phenotypically stable, functional, and confined in the structure. Supplementation of the hydrogel with the Treg-specific bioactive factors interleukin-2 and chemokine ligand 1 improves Treg viability, suppressive phenotype, and function, and attracts to the structure CCR8 + T-cells enriched with anti-inflammatory subpopulations, including Tregs, from human peripheral blood. Furthermore, these findings are applicable to 3D bioprinting. Co-axial printing of murine pancreatic islets with human natural and induced Tregs protects the islets from xenoresponse upon co-culture with human peripheral blood mononuclear cells. This establishes the co-encapsulation of Tregs by co-axial 3D bioprinting as a valid option for providing local immune protection to allogeneic cellular transplants such as pancreatic islets.

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Section: Resultsmentioning

confidence: 99%

Encapsulation of Human Natural and Induced Regulatory T‐Cells in IL‐2 and CCL1 Supplemented Alginate‐GelMA Hydrogel for 3D Bioprinting

Kim

Hope

Gantumur

et al. 2020

Adv Funct Materials

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“…[ 32,43,44 ] In contrary, both electron‐beam irradiation and autoclaving are not recommended: electron‐beam irradiation accelerates the degradation rate of the scaffolds and increases the chemical crosslinking of the material to use, while autoclaving reduces the viscosity and mechanical properties of the materials. [ 43,44 ]…”

Section: Synthetic Bone Graft Materialsmentioning

confidence: 99%

“…Materials are qualitatively ranked from −− (very bad properties) to +++ (excellent properties) based on the literature analysis. [43,44] Synthetic biodegradable polymers PCL, PLGA, PLA + + +++ +++ +++ UV, [56] γ-irradiation, [57] β-irradiation [59] Synthetic non degradable polymers PEEK, PEKK + +++ −− +++ +++ γ-irradiation [70] Ceramics HAP, TCP +++ +/− + − + γ-irradiation [81] Metals Ti ++ ++ −− + − Autoclaving [111,112] that are clinically approved. Biomimetism also includes the osteoconductive and osteoinductive properties of the materials.…”

Section: Synthetic Bone Graft Materialsmentioning

confidence: 99%

Additive Manufacturing of Material Scaffolds for Bone Regeneration: Toward Application in the Clinics

Garot

Bettega

Picart

2020

Adv Funct Materials

139

105

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Additive manufacturing (AM) allows the fabrication of customized bone scaffolds in terms of shape, pore size, material type, and mechanical properties. Combined with the possibility to obtain a precise 3D image of the bone defects using computed tomography or magnetic resonance imaging, it is now possible to manufacture implants for patient‐specific bone regeneration. This paper reviews the state‐of‐the‐art of the different materials and AM techniques used for the fabrication of 3D‐printed scaffolds in the field of bone tissue engineering. Their advantages and drawbacks are highlighted. For materials, specific criteria, are extracted from a literature study: biomimetism to native bone, mechanical properties, biodegradability, ability to be imaged (implantation and follow‐up period), histological performances, and sterilization process. AM techniques can be classified in three major categories: extrusion‐based, powder‐based, and vat photopolymerization. Their price, ease of use, and space requirement are analyzed. Different combinations of materials/AM techniques appear to be the most relevant depending on the targeted clinical applications (implantation site, presence of mechanical constraints, temporary or permanent implant). Finally, some barriers impeding the translation to human clinics are identified, notably the sterilization process.

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“…Using lower energy photons such as UV radiation does not impact printability of alginate but does not fully sterilize the material. 280 Ethylene oxide sterilization has been recommended for gelatin, gelMA, HA, and HAMA 279 and has also been shown to work with alginate but comes with high safety requirements, high cost, 280 and potential long-term health concerns as a carcinogen.…”

Section: Polymers As Bioinks In Bioprintingmentioning

confidence: 99%

“… 282 For bioprinting, sterile filtration caused no change in physicochemical properties and printability for alginate, 280 but for gelMA some high molecular weight fraction was removed, thereby affecting the bioprinting. 279 …”

Section: Polymers As Bioinks In Bioprintingmentioning

confidence: 99%

Emulating Human Tissues and Organs: A Bioprinting Perspective Toward Personalized Medicine

et al. 2020

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The lack of in vitro tissue and organ models capable of mimicking human physiology severely hinders the development and clinical translation of therapies and drugs with higher in vivo efficacy. Bioprinting allow us to fill this gap and generate 3D tissue analogues with complex functional and structural organization through the precise spatial positioning of multiple materials and cells. In this review, we report the latest developments in terms of bioprinting technologies for the manufacturing of cellular constructs with particular emphasis on material extrusion, jetting, and vat photopolymerization. We then describe the different base polymers employed in the formulation of bioinks for bioprinting and examine the strategies used to tailor their properties according to both processability and tissue maturation requirements. By relating function to organization in human development, we examine the potential of pluripotent stem cells in the context of bioprinting toward a new generation of tissue models for personalized medicine. We also highlight the most relevant attempts to engineer artificial models for the study of human organogenesis, disease, and drug screening. Finally, we discuss the most pressing challenges, opportunities, and future prospects in the field of bioprinting for tissue engineering (TE) and regenerative medicine (RM).

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Evaluation of sterilisation methods for bio-ink components: gelatin, gelatin methacryloyl, hyaluronic acid and hyaluronic acid methacryloyl

Abstract: Evaluation of sterilisation methods for bio-ink components: gelatin, gelatin Evaluation of sterilisation methods for bio-ink components: gelatin, gelatin methacryloyl, hyaluronic acid and hyaluronic acid methacryloyl methacryloyl, hyaluronic acid and hyaluronic acid methacryloyl

Cited by 55 publications

References 79 publications

Encapsulation of Human Natural and Induced Regulatory T‐Cells in IL‐2 and CCL1 Supplemented Alginate‐GelMA Hydrogel for 3D Bioprinting

Encapsulation of Human Natural and Induced Regulatory T‐Cells in IL‐2 and CCL1 Supplemented Alginate‐GelMA Hydrogel for 3D Bioprinting

Additive Manufacturing of Material Scaffolds for Bone Regeneration: Toward Application in the Clinics

Emulating Human Tissues and Organs: A Bioprinting Perspective Toward Personalized Medicine

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