Evaluation of Streptococcus mutans adhesion to fluoride varnishes and subsequent change in biofilm accumulation and acidogenicity

Chau, Ngoc Phuong Thanh; Pandit, Santosh; Jung, Jieun; Jeon, Jongho

doi:10.1016/j.jdent.2014.03.009

Cited by 45 publications

(46 citation statements)

References 33 publications

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“…Acid production is an important virulence factor of S. mutans biofilms that warrants attention in studies of dental caries prevention (7). The present results showed that all of the tested materials reduced acid production by S. mutans biofilms during the experimental period; the highest pH was detected in the SDF group.…”

Section: Discussionsupporting

confidence: 48%

“…The process of biofilm development involves initial bacterial adhesion and accumulation, causing enamel demineralization, which indicates that the incidence of dental caries can be reduced by control of biofilm formation and accumulation (4,6,7). Streptococcus mutans is the most cariogenic microorganism in dental biofilm due to its acidogenic and aciduric properties, as well as its capacity to utilize dietary carbohydrates and synthesize extracellular polysaccharides (24,25).…”

Section: Discussionmentioning

confidence: 99%

“…Because biofilm bacteria are the driving force of demineralization and caries development, control of dental plaque has become an important strategy for caries prevention (6)(7)(8).…”

Section: Introductionmentioning

confidence: 99%

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Effects of different antibacterial agents on enamel in a biofilm caries model

et al. 2015

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Using a mature biofilm model, the aim of this study was to evaluate the effectiveness of different antibacterial agents in comparison with silver diamine fluoride (SDF). Forty-eight saliva-coated enamel slabs were inoculated with Streptococcus mutans monospecies biofilm. The biofilms were then exposed to 10% sucrose in tryptone yeast-extract culture medium, 8 times per day for 7 days. After the biofilm growth period, the enamel slabs were treated with one of the following substances: 1) distilled water; 2) SDF; 3) acidulated phosphate fluoride (APF); 4) ammonium hexafluorosilicate (AHF); 5) ammonium hexafluorosilicate + cetylpyridinium chloride (AHF+CPC); or 6) 0.2% chlorhexidine (CHX). After these treatment procedures, the samples were incubated at 37ºC for 2 days, and the numbers of viable microorganisms in the biofilms were counted. The number of viable bacteria was significantly reduced by all of the antibacterial agents (P < 0.05). However, SDF showed the highest antibacterial activity (P < 0.05), and the effectiveness of the other agents was lower (P < 0.05). SDF has a highly effective antibacterial action against cariogenic Streptococcus mutans biofilm; none of the other fluoride agents used in this study, or 0.2 CHX agent, showed an antibacterial effect comparable to that of SDF. (J Oral Sci 57, [367][368][369][370][371][372] 2015)

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Section: Discussionsupporting

confidence: 48%

Section: Discussionmentioning

confidence: 99%

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Effects of different antibacterial agents on enamel in a biofilm caries model

et al. 2015

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“…Among the exopolysaccharides produced by S. mutans, WIG is essential for providing a rigid scaffold for the development of a tenacious biofilm with presenting adhesion-promoting sites [Bowen and Koo, 2011]. Adhesion of bacteria to the tooth's surface is a prerequisite for biofilm formation [Chau et al, 2014]. Gaining an understanding of the adhesion force of dental biofilms to enamel is one key to the development of fundamental knowledge of dental caries [Pelin et al, 2012].…”

Section: The Weakened Biofilm Forming Ability Was Associated With a Rmentioning

confidence: 99%

The Regulator Gene rnc Is Closely Involved in Biofilm Formation in Streptococcus mutans

Mao

Lei

et al. 2018

Caries Res

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Streptococcus mutans is an important factor in the etiology and pathogenesis of dental caries, largely owing to its ability to form a stable biofilm. Previous animal studies have indicated that rnc could decrease the amount of sulcal caries, and that the downregulation of cariogenicity might be due to its capacity to disrupt biofilm formation. However, the biofunctions by which rnc is involved in biofilm formation remain to be elucidated. In this study, we further investigate the role of rnc based on the study of mature biofilm. Scanning electron microscopy and the crystal violet assay were used to detect the biofilm forming ability. The production and distribution of exopolysaccharides within biofilm was analyzed by exopolysaccharide staining. Gel permeation chromatography was used to perform molecular weight assessment. Its adhesion force was measured by atomic force microscopy. The expression of biofilm formation-associated genes was analyzed at the mRNA level by qPCR. Here, we found that rnc could occur and function in biofilm formation by assembling well-structured, exopolysaccharide-encased, stable biofilms in S. mutans. The weakened biofilm forming ability of rnc-deficient strains was associated with the reduction of exopolysaccharide production and bacterial adhesion. Over all, these data illustrate an interesting situation in which an unappreciated regulatory gene acquired for virulence, rnc, most likely has been coopted as a potential regulator of biofilm formation in S. mutans. Further characterization of rnc may lead to the identification of a possible pathogenic biofilm-specific treatment for dental caries.

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“…The interference in one of these steps could prevent the development of the cariogenic biofilm [11][12][13] . Thus, inhibition of the adhesion of S. mutans to enamel surfaces might interfere in the development of a cariogenic biofilm, reducing the potential for dental caries initiation and progression 14 . It was estimated that one-third to one-half of all Americans practice alternative medicine, with use of natural or herbal health care products, especially dentifrices 15 .…”

Section: Introductionmentioning

confidence: 99%

Influence of subinhibitory concentrations of extracts from Psidium cattleianum (Sabine) and Myracrodruon urundeuva (Allemao) on mutans streptococci adhesion to glass and enamel surfaces.

Bianco¹,

Landucci²,

Schweitzer³

et al. 2017

Arch Health Invest

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This study evaluated the influence of subinhibitory concentrations of extracts from Myracrodruon urundeuva and Psidium cattleianum on the adhesion of cariogenic cocci to the surface of glass and bovine enamel. The aqueous extracts were prepared from leaves by heating in deionized water and sterilized by filtration (22μm). The minimum inhibitory concentration (MIC) of the extracts on S. mutans ATCC 1910 and ATCC 35688 was determined by the broth dilution method. In the tests of adhesion to glass surfaces, glass tubes were treated with saliva and sucrose and the bacterial strains were cultivated in concentrations equivalent to 25% of MIC, which were also maintained in the adhesion assay. After the contact between the cells and the glass, the unbound cocci were removed and, finally, it was performed the removal of the cells adhered to the glass by sonication. The percentage of adhesion was determined by comparing the number of bacteria adhered to the total inoculated bacteria, in the presence and absence of the subinhibitory concentrations of the extracts. For enamel adhesion tests, bovine enamel samples were standardized, polished, treated with saliva and, in the test group, received the bacterial inoculum and 25% of the MIC of the extracts. The enamel blocks were removed and the total of adhered cocci was determined by cultivation on BHI agar supplemented with horse blood in anaerobic conditions for 48h at 37 o C. The results were evaluated through analysis of variance (ANOVA). The extracts were effective in inhibiting bacterial adhesion to glass and dental enamel surfaces, although inhibition varied according to bacterial strain and extract, producing from 40 to 60% of inhibition. The results reinforce the possibility of the use of extracts of plants of the Brazilian savanna in the control of cariogenic biofilms.Descriptors: Bacteria; Prevention & Control; Plant Extracts; Dental Caries. ResumoEste estudo avaliou a influência de concentrações subinibitórias dos extratos de Myracrodruon urundeuva e Psidium cattleianum sobre a adesão de cocos cariogênicos à superfície do vidro e esmalte bovino. Os extratos aquosos foram preparados a partir de folhas, por aquecimento em água deionizada e esterilizados por filtração (22µm). A seguir, a concentração inibitória mínima (CIM) dos extratos sobre S. mutans ATCC 1910 e ATCC 35688 foi determinada através do método de diluição da droga em caldo. Nos testes de aderência ao vidro, tubos eram tratados com saliva e sacarose e as cepas bacterianas eram cultivadas em concentrações equivalentes à 25% da CIM, que também eram mantidas no ensaio de adesão. Após o contato entre as células o do vidro, fazia-se a remoção dos cocos não aderidos e, posteriormente, por sonicação, a remoção das células aderidas ao vidro. A percentagem de adesão era determinada comparando-se o número de bactérias aderidas em relação ao total, na presença e ausência das concentrações subinibitórias dos extratos. Para adesão ao esmalte, amostras de esmalte bovino foram padronizadas, polidas, tratadas com...

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Evaluation of Streptococcus mutans adhesion to fluoride varnishes and subsequent change in biofilm accumulation and acidogenicity

Cited by 45 publications

References 33 publications

Effects of different antibacterial agents on enamel in a biofilm caries model

Effects of different antibacterial agents on enamel in a biofilm caries model

The Regulator Gene <b><i>rnc</i></b> Is Closely Involved in Biofilm Formation in <b><i>Streptococcus mutans</i></b>

Influence of subinhibitory concentrations of extracts from Psidium cattleianum (Sabine) and Myracrodruon urundeuva (Allemao) on mutans streptococci adhesion to glass and enamel surfaces.

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