Evaluation of Streptococcus mutans biofilms formed on fluoride releasing and non fluoride releasing resin composites

Relationship between fluoride release rate and anti-cariogenic biofilm activity of glass ionomer cements

“…In this study, we investigated acidogenicity and composition of a cariogenic biofilm on GICs. Furthermore, we determined the relationship between anti-cariogenic biofilm activity of GICs and their fluoride release rate since (i) it has been well demonstrated that fluoride ions, even in low concentrations, can affect the virulence of cariogenic biofilms [21], and (ii) many GICs release fluoride ions into dental biofilms and teeth [17,28,29]. In this study, we used an S. mutans biofilm model to study acidogenicity and composition of cariogenic biofilms.…”

“…S. mutans biofilms were formed on saliva-coated GIC or hydroxyapatite (HA) discs placed in a vertical position in 24-well plates, as detailed elsewhere [21]. Briefly, the saliva-coated discs were generated by incubation with filtersterilized (0.22 m low protein-binding filter) human whole saliva for 1 h at 37 • C. For biofilm formation, the saliva-coated discs were transferred to a 24-well plate containing 1% sucrose (v/v) ultrafiltered (10 kDa molecular-weight cut-off) tryptone yeast-extract (UTE) broth with S. mutans UA159 (2-5 × 10 6 colony forming units (CFUs)/ml).…”

“…Recently, several studies have examined the effects of fluoride-releasing materials on acidogenicity of cariogenic bacteria [13,21,29]. Some investigations demonstrated that fluoride-releasing materials can affect the acidogenicity of cariogenic biofilms.…”

Chitosan microparticles for the controlled delivery of fluoride

“…These include the sensitisation of biofilms to acid damage, reduction of acid production through the inhibition of enzymes involved in glycolysis, reduction of IgA1 protease synthesis, the disruption of bacterial adherence and the reduction in extracellular polysaccharide production. [7][8][9][10][11][12] The incorporation of fluoride into dental materials has been shown to inhibit the growth of relevant bacteria. 12,13 Moreover, such materials can be ''recharged'' with fluoride, 14 and the continuous presence of even low concentrations of fluoride in the fluid phase surrounding the teeth is considered to confer significant cariostatic benefits.…”

“…[7][8][9][10][11][12] The incorporation of fluoride into dental materials has been shown to inhibit the growth of relevant bacteria. 12,13 Moreover, such materials can be ''recharged'' with fluoride, 14 and the continuous presence of even low concentrations of fluoride in the fluid phase surrounding the teeth is considered to confer significant cariostatic benefits. 15,16 The use of oral care products containing fluoridated actives such as sodium fluoride (NaF), amine fluoride (AmF 2 ) and stannous fluoride (SnF 2 ) result in elevated fluoride concentrations both in saliva and plaque fluid for up to several hours.…”