Evaluation of superficially porous particle based zwitterionic chiral ion exchangers against fully porous particle benchmarks for enantioselective ultra-high performance liquid chromatography

Geibel, Christian; Dittrich, Kristina; Woiwode, Ulrich; Kohout, Michal; Zhang, Tong; Lindner, Wolfgang; Lämmerhofer, Michael

doi:10.1016/j.chroma.2019.06.026

Cited by 33 publications

(33 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recently, it has been shown that particle and pore size play a crucial role in the chromatographic performance of anion‐exchange and zwitterion ion‐exchange CSPs based on a quinine selector. For both FPP and SPP, it has been documented that larger pores are beneficial for column efficiency due to better diffusion characteristics of such CSPs . However, not only the type of the silica particle is important, but also the total selector coverage and surface morphology of the modified particle significantly contribute to the overall chromatographic performance of a CSP .…”

Section: Resultsmentioning

confidence: 99%

Evaluation of silica from different vendors as the solid support of anion‐exchange chiral stationary phases by means of preferential sorption and liquid chromatography

Kohout

Hovorka

Herciková

et al. 2019

J of Separation Science

Self Cite

View full text Add to dashboard Cite

Chromatographic performance of a chiral stationary phase is significantly influenced by the employed solid support. Properties of the most commonly used support, silica particles, such as size and size distribution, and pore size are of utmost importance for both superficially porous particles and fully porous particles. In this work, we have focused on evaluation of fully porous particles from three different vendors as solid supports for a brush‐type chiral stationary phase based on 9‐O‐tert‐butylcarbamoyl quinidine. We have prepared corresponding stationary phases under identical experimental conditions and determined the parameters of the modified silica by physisorption measurements and scanning electron microscopy. Enantiorecognition properties of the chiral stationary phases have been studied using preferential sorption experiments. The same material was slurry‐packed into chromatographic columns and the chromatographic properties have been evaluated in liquid chromatography. We show that preferential sorption can provide valuable information about the influence of the pore size and total pore volume on the interaction of analytes of different size with the chirally‐modified silica surface. The data can be used to understand differences observed in chromatographic evaluation of the chiral stationary phases. The combination of preferential sorption and liquid chromatography separation can provide detailed information on new chiral stationary phases.

show abstract

Section: Resultsmentioning

confidence: 99%

Evaluation of silica from different vendors as the solid support of anion‐exchange chiral stationary phases by means of preferential sorption and liquid chromatography

Kohout

Hovorka

Herciková

et al. 2019

J of Separation Science

Self Cite

View full text Add to dashboard Cite

show abstract

“…For this work, molecular dynamics simulations, lipophilicity/hydrophilicity measurements of selectors, pH-dependent ζ-potential determinations and chromatographic characterization were studied for both columns [88]. Geibel et al have demonstrated the Chiralpak ZWIX column with superficially-porous particles (SPP) allowed a better separation performance than a Chiralpak ZWIX column with fully-porous particles (FPP) [89].…”

Section: Ion-and Zwitterion-exchange-based Chiral Stationary Phasesmentioning

confidence: 99%

Recent Advances in Chiral Analysis of Proteins and Peptides

Morvan

Mikšı́k

2021

Separations

View full text Add to dashboard Cite

Like many biological compounds, proteins are found primarily in their homochiral form. However, homochirality is not guaranteed throughout life. Determining their chiral proteinogenic sequence is a complex analytical challenge. This is because certain D-amino acids contained in proteins play a role in human health and disease. This is the case, for example, with D-Asp in elastin, β-amyloid and α-crystallin which, respectively, have an action on arteriosclerosis, Alzheimer's disease and cataracts. Sequence-dependent and sequence-independent are the two strategies for detecting the presence and position of D-amino acids in proteins. These methods rely on enzymatic digestion by a site-specific enzyme and acid hydrolysis in a deuterium or tritium environment to limit the natural racemization of amino acids. In this review, chromatographic and electrophoretic techniques, such as LC, SFC, GC and CE, will be recently developed (2018–2020) for the enantioseparation of amino acids and peptides. For future work, the discovery and development of new chiral stationary phases and derivatization reagents could increase the resolution of chiral separations.

show abstract

“…SPP-based bonded CPs include isopropyl cyclofructan 6 (Spudeit et al, 2014), cylcofructan-7 (Patel et al, 2015), naproxen selector (Ismail et al, 2016), cellulose 3,5-dichlorophenylcarbamate (Bezhitashvili et al, 2018), anion-exchange-type chiral selector, tert-butylcarbamoylquinine (Schmitt et al, 2018), and zwitterionic chiral ion-exchange selectors obtained by conjugation of quinine and 2-aminocyclohexanesulfonic acid via a carbamate (Geibel et al, 2019).…”

Section: Chiral Separationsmentioning

confidence: 99%

Fundamental to achieving fast separations with high efficiency: A review of chromatography with superficially porous particles

Luo

DeStefano

Langlois

et al. 2021

Biomedical Chromatography

View full text Add to dashboard Cite

Types of particles have been fundamental to LC separation technology for many years. Originally, LC columns were packed with large-diameter (>100 μm) calcium carbonate, silica gel, or alumina particles that prohibited fast mobile-phase speeds because of the slow diffusion of sample molecules inside deep pores. During the birth of HPLC in the 1960s, superficially porous particles (SPP, ≥30 μm) were developed as the first high-speed stationary-phase support structures commercialized, which permitted faster mobile-phase flowrates due to the fast movement of sample molecules in/out of the thin shells. These initial SPPs were displaced by smaller totally porous particles (TPP) in the mid-1970s. But SPP history repeated when UHPLC emerged in the 2000s. Stationary-phase support structures made from sub-3-μm SPPs were introduced to chromatographers in 2006. The initial purpose of this modern SPP was to enable chromatographers to achieve fast separations with high efficiency using conventional HPLCs. Later, the introduction of sub-2-μm SPPs with UHPLC instruments pushed the separation speed and efficiency to a very fast zone.This review aims at providing readers a comprehensive and up-to-date view on the advantages of SPP materials over TPPs historically and theoretically from the material science angle.

show abstract

Evaluation of superficially porous particle based zwitterionic chiral ion exchangers against fully porous particle benchmarks for enantioselective ultra-high performance liquid chromatography

Cited by 33 publications

References 54 publications

Evaluation of silica from different vendors as the solid support of anion‐exchange chiral stationary phases by means of preferential sorption and liquid chromatography

Evaluation of silica from different vendors as the solid support of anion‐exchange chiral stationary phases by means of preferential sorption and liquid chromatography

Recent Advances in Chiral Analysis of Proteins and Peptides

Fundamental to achieving fast separations with high efficiency: A review of chromatography with superficially porous particles

Contact Info

Product

Resources

About