Evaluation of surface structural and mechanical changes following remineralization of dentin

Bertassoni, Luiz E.; Habelitz, Stefan; Pugach, Megan K.; Soares, Paulo; Marshall, Sally J.; Marshall, Grayson W.

doi:10.1002/sca.20199

Cited by 70 publications

(70 citation statements)

References 28 publications

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“…4C·II). This finding is consistent with fibrils containing intrafibrillar and extrafibrillar mineral [49,50] . Collagen regulates mineralization, and a single mutation on a gen that encodes for an α-chain can be responsible for osteogenesis imperfect and dentinogenesis imperfect type I [34] .…”

Section: Resultssupporting

confidence: 88%

Ex vivo detection and characterization of remineralized carious dentin, by nanoindentation and single point Raman spectroscopy, after amalgam restoration

Osorio

Aguilera

Cabello

et al. 2016

J Raman Spectroscopy

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The aim of this study was to assess the mechanical and chemical performance of sound and caries-affected dentin (CAD), after Zn-free vs containing amalgam restorations placement and thermocycling. Dentin surfaces were studied by Atomic Force Microscopy (AFM) analysis for surface morphological characterization (including fibril diameter assessment), nano-indentation (to measure nano-hardness-Hi and modulus of Young-Ei), and single point Raman spectroscopy for chemical analysis. Measurements were performed before amalgam placement, after amalgam removal, and after 3 months of thermocycling (100,000cy/5 ºC and 55 ºC). Restorations increased both Hi and Ei at intertubular dentin of CAD. The highest values of Hi were achieved at intertubular dentin after restoring with Zn-containing amalgams. Remineralization of dentin was attributed to the increase of both amorphous and crystalline new mineral, as lower degrees of crystal imperfections in junction with crystal disorders, and improvement in structural stability of collagen were found. Higher presence of minerals were also confirmed after the decrease of fluoridated apatite and the increase of the total water content. Proteoglycans, lipids, and proteins, augmented in both sound and CAD, providing support for the mineral growing. The increase of bands assigned to vibration of carbonate calcium phosphate contributed to a decrease of crystallinity.

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Section: Resultssupporting

confidence: 88%

Ex vivo detection and characterization of remineralized carious dentin, by nanoindentation and single point Raman spectroscopy, after amalgam restoration

Osorio

Aguilera

Cabello

et al. 2016

J Raman Spectroscopy

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“…This typical staggered pattern of collagen fibrils (Figs 9·bI, 9·cII) was not visible in the partially demineralized dentin of CAD (Fig 9a), but was evident when this substrate became clearly remineralized (Fig 9c). This finding is consistent with fibrils containing intrafibrillar or extrafibrillar mineral 61,63 .…”

supporting

confidence: 88%

On modeling and nanoanalysis of caries-affected dentin surfaces restored with Zn-containing amalgam and in vitro oral function

et al. 2015

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The aim of this research was to assess the influence of mechanical loading on the ability of Zn-free vs Zn-containing amalgams to promote remineralization at the dentin interface. Sound (SD) and caries-affected dentin surfaces (CAD) were restored using Zn-free or Zn-containing dental amalgams. Mid-coronal dentin surfaces were studied by 1) Atomic Force Microscopy (AFM) analysis (including plot and phase imaging, nanoindentation test (modulus of Young (Ei), nano-roughness measurements, and fibril diameter assessment), 2) Raman spectroscopy/cluster analysis, 3) X-ray diffraction (µXRD 2 ), 4) field emission electron microscope (FESEM) and energy-dispersive analysis (EDX), for morphological, mechanical, and physico-chemical characterization.Analyses were performed before amalgam placement and after amalgam removal, at 24 hours and 3 weeks of load cycling. Zn-free and Zn-containing amalgams restorations promoted an increase in the modulus of Young of CAD surfaces, after three weeks of load cycling; at this time, Zn-containing amalgams attained higher Ei than Zn-free restorations. Zn-containing amalgams induced tubular occlusion after load cycling, in both sound and CAD. Zn free-amalgams promoted remineralization of both intertubular and peritubular dentin in CAD substrata. These minerals were identified as calciumphosphate deposits, and crystals as hydroxyl-apatite with augmented crystallographic maturity but with some components of lattice distortion. Crosslinking of collagen diminished and secondary structure of collagen increased in CAD substrate restored with Zn-containing amalgam after 3 w of load cycling, indicating an advanced preservation, molecular organization and orientation of collagen fibrils after load cycling. Plot and phase images permitted to observe the topographical changes which were promoted by the mineral deposits; in general, the indexes related to higher remineralization gave rise to a decrease of nano-roughness and an augmentation of the 4 bandwidth of the collagen fibrils. Zn-containing amalgam restorations submitted to mechanical stimuli promote remineralization of the partially mineral-depleted subjacent substrate at the caries-affected dentin.

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“…The increase in the percentage of adhesive failures may be interpreted as a result of the strengthening of the resin-dentin interface from remineralization . Moreover, fibrils had a high width (100 to 200 nm), which usually occurs when intrafibrillar mineralization exists or cross-linking is maintained (Bertassoni et al, 2010;Misra et al, 2004). In general terms, phosphate and carbonate peak heights and areas increased at the interface when NPs were used for dentin infiltration, but peaks and ratios concerning the organic components (crosslinking and nature/secondary structure of collagen) decreased after 21 d of immersion (Tables 3 and 4).…”

Section: Discussionmentioning

confidence: 99%

Ions-modified nanoparticles affect functional remineralization and energy dissipation through the resin-dentin interface

Toledano

Osorio

et al. 2017

Journal of the Mechanical Behavior of Biomedical Materials

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The aim of this study was to evaluate changes in the mechanical and chemical behavior, and bonding ability at dentin interfaces infiltrated with polymeric nanoparticles (NPs) prior to resin application. Dentin surfaces were treated with 37% phosphoric acid followed by application of an ethanol suspension of NPs, Zn-NPs or Ca-NPs followed by the application of an adhesive, Single Bond (SB). Bonded interfaces were stored for 24 h, submitted to microtensile bond strength test, and evaluated by scanning electron microscopy. After 24 h and 21 d of storage, the whole resin-dentin interface adhesive was evaluated using a Nano-DMA. Complex modulus, storage modulus and tan delta (δ) were assessed. AFM imaging and Raman analysis were performed. Bond strength was not affected by NPs infiltration. After 21 d of storage, tan δ generally decreased at Zn-NPs/resin-dentin interface, and augmented when Ca-NPs or non-doped NPs were used. When both Zn-NPs and Ca-NPs were employed, the storage modulus and complex modulus decreased, though both moduli increased at the adhesive and at peritubular dentin after Zn-NPs infiltration. The phosphate and the carbonate peaks, and carbonate substitution, augmented more at interfaces promoted with Ca-NPs than with Zn-NPs after 21 d of storage, but crystallinity did not differ at created interfaces with both ions-doped NPs. Crosslinking of collagen and the secondary structure of collagen improved with Zn-NPs resin-dentin infiltration. Ca-NPs-resin dentin infiltration produced a favorable dissipation of energy with minimal stress concentration trough the crystalline remineralized resin-dentin interface, causing minor damage at this structure.

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Evaluation of surface structural and mechanical changes following remineralization of dentin

Cited by 70 publications

References 28 publications

Ex vivo detection and characterization of remineralized carious dentin, by nanoindentation and single point Raman spectroscopy, after amalgam restoration

Ex vivo detection and characterization of remineralized carious dentin, by nanoindentation and single point Raman spectroscopy, after amalgam restoration

On modeling and nanoanalysis of caries-affected dentin surfaces restored with Zn-containing amalgam and in vitro oral function

Ions-modified nanoparticles affect functional remineralization and energy dissipation through the resin-dentin interface

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