Evaluation of TaGSK1 Gene Expression in Selected Wheat Genotypes as Salinity Marker Assisted Selection

Determination ofTaGSK1 gene expression in selected wheat under different salt stresses

“…The results of this study showed that TaGSK1 gene was expressed in the salt-tolerant cultivars (Dijila and Furat) and the genotype (2H) but not expressed in salt-sensitive cultivar (Tamooze 2). These results are in agreement with the results of (Chen et al, 2003, Bahrami et al, 2009. They indicated the importance of this gene as wheat salt-resistance marker.…”

“…The results also showed that TaGSK1 gene expression increased with increase of salt concentration. These results indicated that TaGSK1 gene is very important in salt tolerance as its expression incearesed with salt stress increase (Chen et al, 2003: Bahrami et al, 2009Guang et al, 2011;. The result also indicated that B-actin gene was suitable as a reference gene for the used plant cultivars due to its stablity at specific CT value during SYBR green real time PCR in salt resistance and sensitive wheat plants.…”

“…Its was induced by NaCl stress and its expression level was more strongly in salt-tolerant mutant than salt-sensitive genotypes as indicated by Northern blot, suggesting it may be involved in signal transduction of salt-stress in wheat (Chen et al, 2003). In addition, TaGSK1 expression was used as salt tolerance marker in wheat (Bahrami et al, 2009). The aim of this study is estimate the TaGSK1 gene expression using SYBR real time PCR for some selected wheat cultivated in Iraq.…”

Estimation of (TaGSK1) gene expression related with salt tolerance in four bread wheat cultivars (Triticum aestivum L.)

“…As show in Table (4) and Figure (4), Furat and Dijila cultivares gave the highest expression, reached to (8.491 x 10 15 and 8.379 x 10 15 ) respectively at the highest salinity level (20 ds/m), while the control treatments recorded (1.716 x 10 12 and 1.520 x 10 12 ) respectively, whereas Lateffyia and Tamooze-2 did not showed any expression of TaGSK1gene under the same condtions. These results corresponded with Bahrami et al (2009) and Fakheri et al (2015), they reported that TaGSK1 gene expresion was not detectable under control treatment, also pointed that this gene controls some tolerance mechanisms, support salt tolerance genotypes to growth normally and consider as a marker for salt tolerance. Chen et al (2003) revealed that the TaGSK1 gene is one of serine-threonine protein kinase that gave the highest gene expression under salinity conditions.…”