Evaluation of the advantages of platelet concentrates stored at 4°C versus 22°C

Yang, Jiangcun; Yin, Wen; Zhang, Yali; Sun, Yang; Ma, Ting; Gu, Shunli; Gao, Ying; Zhang, Xiaole; Yuan, Jun; Wang, Wenhua

doi:10.1111/trf.14462

Cited by 18 publications

(23 citation statements)

References 38 publications

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“…These findings suggest that looking at α‐synuclein concentration increases as a surrogate biomarker of changes to platelet biology in response to such technology may prove essential to determine if use of pathogen reduction leads to changes in platelets’ health. Similarly, in light of the current increased interest in using cold‐stored platelets, it would also be of interest to establish whether α‐synuclein concentration changes can also be indicative of platelet health in this setting as well. These areas should be investigated in the future studies.…”

Section: Discussionmentioning

confidence: 99%

α‐Synuclein concentration increases over time in plasma supernatant of single donor platelets

Stefaniuk

Hong

Maitta

2018

European J of Haematology

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Section: Discussionmentioning

confidence: 99%

α‐Synuclein concentration increases over time in plasma supernatant of single donor platelets

Stefaniuk

Hong

Maitta

2018

European J of Haematology

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“…Based on the results of previous studies, [22–24] the correction effect of PLT preservation in cold storage in the in vitro model of thrombocytopenia [25] has been verified. Thromboelastography (TEG) indices and routine blood indices were used to evaluate blood coagulation function, anemia corrective effect, and PLT counts on an in vitro model of massive blood loss and thrombocytopenia (see experimental flow chart; Fig.…”

Section: Methodsmentioning

confidence: 94%

“…[17,18,19] Some investigations reported that PLTs in whole blood stored at low temperatures could survive for 10 days in the circulation, [20,21] and could be preserved for 5 to 7 days, and 14 days in vitro. [19,22,23] The results of a series of experiments that we conducted on PLTs stored at 4°C in vitro were as follows [24] : PLTs stored at 4°C for 10 to 14 days showed better PLT count, cellular morphology, and PLT membrane structure and cytoplasm than PLTs stored at 22°C for 5 days; PLTs stored at 4°C had slower metabolism, including less production of lactic acid, slower decrease of pH, lower consumption of glucose, and a higher recovery rate of the PLT hypotonic shock reaction rate compared with PLTs stored at 22°C within the same storage period; PLTs stored at 4°C had higher degree of aggregation, a stronger hemostatic function, and a higher activation state than the latter, and could be preserved for 10 to 14 days.…”

Section: Introductionmentioning

confidence: 99%

Good hemostatic effect of platelets stored at 4°C in an in vitro model of massive blood loss and thrombocytopenia

Yang

Sun

et al. 2019

Medicine

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This study compared the corrective effects of storage of platelets at 4°C and at 22°C in an in vitro model of massive blood loss and thrombocytopenia to provide an experimental basis for the storage of platelets for clinical applications. In vitro model of massive blood loss and thrombocytopenia were constructed by the in vitro hemodilution method and cell washing method. Using storage of platelets at 4°C (1, 3, 5, 7, 10, 14 days) and at 22°C (1, 3, 5 days) to correct the coagulation condition of the different models, by thromboelastography and by routine blood indices. ①Platelets stored at 4°C (1, 3, 5,7, 10, 14 days) and at 22°C (1, 3, 5 days) to correct the in vitro model of massive blood loss. Platelet count results improved from 17 to 27 × 10 9 /L to greater than 120 × 10 9 /L for 4°C storage, and 20 to 27 × 10 9 /L to greater than 120 × 10 9 /L for 22°C storage. Thromboelastography maximum amplitude (TEG-MA) results improved from 8.8 to 15.4 mm to greater than 43 mm for 4°C storage, and 12.2 to 14.4 mm to greater than 44.8 mm for 22°C storage. Thromboelastography reaction time values decreased from 9.9–24.9 minutes to 3.8–5.5 minutes for 4°C storage, and 9.9–22.7 minutes to 4.3–4.5 minutes for 22°C storage. ②Platelets stored at 4°C (1, 3, 5,7, 10, 14 days) and at 22°C (1, 3, 5 days) to correct the in vitro model of thrombocytopenia. Platelet count results improved from 12 to 34 × 10 9 /L to greater than 99 × 10 9 /L for 4°C storage, and 12 to 34 × 10 9 /L to greater than 120 × 10 9 /L for 22°C storage. TEG-MA results improved from 21.4 to 32.1 mm to greater than 49.1 mm for 4°C storage, and 21.4 to 31.6 mm to greater than 50.5 mm for 22°C storage. Platelets stored at 4°C and 22°C have the same correcting effect for 1, 3, and 5 days. Platelets stored at 4°C for 7 to 14 days have similarly hemostatic effect on the in vitro model of massive blood loss and thrombocytopenia.

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“…Expression of activated forms of GPIIb‐IIIa have been evaluated in different PCs using a specific antibody (PAC‐1). Results of several studies indicate that PAC‐1–positive platelets are significantly increased in different platelet products, indicating that they have been preactivated . Reduced platelet aggregation should be expected for platelet products showing an enhanced binding of PAC‐1.…”

Section: In Vitro Testsmentioning

confidence: 99%

Platelet in vitro assays: their correspondence with their in vivo hemostatic potential

Escolar

McCullough

2019

Transfusion

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Developments during the past few years have resulted in multiple kinds of platelet products for transfusion. This involves different collection methods, containers, preservative solutions, modifications of storage temperatures and durations, and additional treatments such as pathogen reduction. Much experience has been obtained testing these processes in vitro to seek indications of in vivo effectiveness. Availability of an in vitro method that correlated with in vivo effectiveness would be extremely valuable for these different kinds of platelet products and as more innovation in platelet preparation occurs in the future. This report reviews the methods for in vitro platelet testing with a view to their in vivo implications and whether such testing could be helpful in projecting the clinical effectiveness of different platelet products. ABBREVIATIONS: ADP = adenosine diphosphate; ATP = adenosine triphosphate; GP = glycoprotein; PAR = proteaseactivated receptor; PASs = platelet additive solutions; PCs = platelet concentrates; PRT = pathogen reduction technology; TEG = thromboelastography; TRAP = thrombin-activated receptor peptide; VWF = von Willebrand factor. From the

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Evaluation of the advantages of platelet concentrates stored at 4°C versus 22°C

Abstract: PLT counts, cellular morphologies, PLT membranes, cytoplasmic structures, aggregation rates, and hemostatic PLT function stored at 4°C for 10 to 14 days were better than those stored at 22°C for 5 days.

Cited by 18 publications

References 38 publications

α‐Synuclein concentration increases over time in plasma supernatant of single donor platelets

α‐Synuclein concentration increases over time in plasma supernatant of single donor platelets

Good hemostatic effect of platelets stored at 4°C in an in vitro model of massive blood loss and thrombocytopenia

Platelet in vitro assays: their correspondence with their in vivo hemostatic potential

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