Evaluation of the anti-proliferation and anti-migration effects of <i>Leucaena leucocephala</i> and <i>Dolichandrone serrulata</i> ethanolic extracts against human cervical cancer cell line

Chatchanayuenyong, Ratikorn; Sujayanont, Patcharawan

doi:10.4103/pm.pm_327_19

Cited by 7 publications

(7 citation statements)

References 16 publications

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“…In addition, phytosteroids, especially terpenoids, detected in the ethanolic extract of D. serrulata barks by using Folin–Ciocalteu analysis (Khaing et al., 2018). Chatchanayuenyong and Sujayanont (2020) have also shown antioxidant activity using DPPH and FRAP analyses. Based on NMR analyses, methanolic D. serrulata branch extract contained various antioxidants including phenolic triglycoside, dolichandroside, decaffeoyl‐verbascoside, verbascoside, isoverbascoside, markhamioside A, 2″‐O‐apiosylverbascoside, luteoside B and ixoside (Sinaphet et al., 2006).…”

Section: Discussionmentioning

confidence: 95%

“…ex DC.) extracted by ethanol or methanol have been shown to have the antioxidant capacities (Chatchanayuenyong & Sujayanont, 2020; Khaing et al., 2018; Kiratipaiboon et al., 2012; Sinaphet et al., 2006). Its barks and roots were shown to have antioxidant substances such as alkaloids, flavonoids, glycosides, phenolic compounds, saponins and tannins (Khaing et al., 2018; Kiratipaiboon et al., 2012).…”

Section: Discussionmentioning

confidence: 99%

“…In this study, aqueous DSF contained total phenolic compounds (27.48 ± 0.34 μg GAE/g DSF) and flavonoid content (5.85 ± 0.07 mg Catechin/g DSF) and possessed antioxidant activities as determined by DPPH (0.53 ± 0.00 mg/ml) and FRAP (153.88 ± 0.68 μmol of Fe (II)/1g DSF). In contrast, ethanol DSF extract showed the highest content of TPC (34.796 ± 1.994 μg GAE/mg extract), flavonoid content (8.038 ± 2.642 μg of QE equivalent/mg extract), and antioxidant activities by DPPH assay (1.272 ± 0.345 mM of TAE/mg extracts) and FRAP value (0.215 ± 0.003 mM Fe (II)/mg) extracts (Chatchanayuenyong & Sujayanont, 2020). Indeed, the antioxidant contents and activities measured in our study were lower than those of previous ethanol DSF extract because bioactive substances especially flavonoid and total phenolic compounds are well extracted in semi‐polar solvents such as ethanol and methanol.…”

Section: Discussionmentioning

confidence: 99%

“…Interestingly, it has been reported that DSF extract increased body weight and had no toxicity on vital organs in mice by single administration (Katisart & Konsue, 2019). Currently, it was revealed that DSF extract contains antioxidant, has anti‐migration and anti‐proliferative effect on cancer cells (Chatchanayuenyong & Sujayanont, 2020).…”

Section: Introductionmentioning

confidence: 99%

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The effect of Dolichandrone serrulata (wall. ex DC.) Seem. flower extract containing antioxidant capacity and terpenoids on the male reproductive system

et al. 2021

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Although the fruit extract of Dolichandrone genus was shown to inhibit spermatogenesis, the reproductive toxicity of Dolichandrone serrulata flowers (DSFs) is not documented. Recent study aimed to evaluate the sub‐chronic toxicity of DSF on male reproductive system. Antioxidant capacity and total phenolic contents of DSF extract were determined using Folin–Ciocalteu's, 2,2‐diphenyl‐1‐picrylhydrazyl and ferric reducing antioxidant power assays. The terpenoid components were determined using nuclear magnetic resonance spectrum. Adult male rats were treated orally with DSF (100, 300 or 600 mg/kg) for 48 days. Histopathology of testis and epididymis was observed. Sperm concentration, viability, acrosome status and morphology were also examined. Expressions of heat shock protein 70 (Hsp70), tyrosine‐phosphorylated (TyrPho) proteins, androgen receptor (AR) and steroidogenic acute regulatory (StAR) protein in testis were investigated. Results showed that DSF contained phenolic compounds and terpenoids (phytoandrogens; rengyolone and cleroindicin B). No reproductive histopathology was observed in DSF‐treated rats. Although DSF decreased the serum testosterone level, the sperm qualities were not affected. Particularly, sperm concentration of DSF‐treated animals was significantly increased. DSF changed the testicular TyrPho proteins but the expression of AR, StAR or Hsp70 was not altered. In conclusion, DSF possesses antioxidant capacity with no toxicity on male reproductive system.

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Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The effect of Dolichandrone serrulata (wall. ex DC.) Seem. flower extract containing antioxidant capacity and terpenoids on the male reproductive system

et al. 2021

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“…The hexane fraction of D. serrulata flower extracts contained total flavonoid contents suggesting antioxidant activity with anti-cancer capacity. 20 An in vivo study revealed that D. serrulata flower extracts decreased the free radical indicator malondialdehyde (MDA) in type 2 diabetic rats. 21 The antioxidant activity of this plant may be due to the presence of secondary metabolites such as phenolic compounds and flavonoids that are responsible for free radical scavenging effects.…”

Section: α-Glucosidase Inhibitory Activitymentioning

confidence: 99%

Antioxidant and ?-Glucosidase Inhibitory Activities of Dolichandrone serrulata Extracts

2021

TJNPR

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Dolichandrone serrulata (Bignoniaceae) is used as a vegetable and food ingredient by the local people in the northern and eastern parts of Thailand. The antioxidant and α-glucosidase inhibitory activities of D. serrulata extracts were investigated. Three parts of D. serrulata (the flowers, leaves and pods) were extracted using water and 95% ethanol. The antioxidant activity of the extracts was examined by DPPH, ABTS and FRAP assays. The total phenolic content (TPC) and total flavonoid content (TFC) were determined by the colorimetric method. The αglucosidase inhibition assay was used to evaluate the in vitro anti-diabetic activity of the extracts. Results revealed that the extracts contained phenolic compounds and flavonoids. Aqueous pod extracts had the highest TPC and TFC (0.245 ± 0.020 mg GAE/g and 0.1102 ± 0.0033 mg QE/g, respectively), while aqueous leaf extracts had the highest antioxidant capacity against DPPH and ABTS (IC50 = 0.12 ± 0.020 and 0.05 ± 0.006 mg/mL, respectively). Ethanol leaf extracts had the highest FRAP value (0.988 ± 0.236 mg TE/g), while ethanol pod extracts had the highest α-glucosidase inhibitory activity (IC50 = 0.041 ± 0.002 mg/mL). In conclusion, aqueous pod extracts from D. serrulata displayed the highest TPC and TFC and antioxidant capacity against DPPH and ABTS, while ethanol leaf extracts had the highest antioxidant activity by FRAP assay. Fruit pod ethanol extracts exhibited α-glucosidase inhibitory activity, suggesting preliminary anti-diabetic activity possibly due to high phenolic and flavonoid contents.

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Ethanolic Fruit Extract of Piper Retrofractum Induced Cell Cycle Arrest, Apoptosis, Increased Reactive Oxygen Species Production, and Mitochondrial Dysfunction in Human Cervical Cancer

Chaisri,

Laoprom,

Buranrat

2024

Chemistry & Biodiversity

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Cervical cancer remains a significant global health challenge, with high mortality rates and a pressing need for more effective therapeutic options. This study investigates the effects of an ethanolic extract from the fruit of Piper retrofractum (PR) on proliferation and apoptosis in cervical cancer cells. We evaluated the cytotoxicity of the crude ethanolic extract of PR using the sulforhodamine B and colony formation assay. ROS generation, apoptosis, and mitochondrial function were quantified via flow cytometry. The effects on cell migration was assessed using wound healing and Transwell migration assays. The PR extract exhibited a significant inhibitory effect on HeLa cell viability, leading to reduced cancer cell proliferation. The extract induced cell cycle arrest at the G0/G1 phase in a dose‐dependent manner and decreased the proportion of cells in the G2/M phase at concentrations of 100 and 250 µg/mL. Additionally, treatment with the PR extract resulted in a marked increase in ROS production, disruption of mitochondrial function, and inhibition of cell migration. These findings suggest that the PR ethanolic fruit extract exerts substantial antiproliferative, antimigratory, and proapoptotic effects on HeLa cells. Consequently, the PR ethanolic fruit extract holds promise as a potential novel therapeutic agent for the treatment of cervical cancer.

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Evaluation of the anti-proliferation and anti-migration effects of Leucaena leucocephala and Dolichandrone serrulata ethanolic extracts against human cervical cancer cell line

Cited by 7 publications

References 16 publications

The effect of Dolichandrone serrulata (wall. ex DC.) Seem. flower extract containing antioxidant capacity and terpenoids on the male reproductive system

The effect of Dolichandrone serrulata (wall. ex DC.) Seem. flower extract containing antioxidant capacity and terpenoids on the male reproductive system

Antioxidant and ?-Glucosidase Inhibitory Activities of Dolichandrone serrulata Extracts

Ethanolic Fruit Extract of Piper Retrofractum Induced Cell Cycle Arrest, Apoptosis, Increased Reactive Oxygen Species Production, and Mitochondrial Dysfunction in Human Cervical Cancer

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