Evaluation of the anticoagulant properties of aprotinin in vitro

Abstract

The interaction of aprotinin with normal coagulation was studied in blood samples obtained from 10 healthy subjects. Each sample was simultaneously tested in four different preparations: NaCl-treated blood: 0.03 mL 0.9% NaCl in 0.33 mL blood; aprotinin treated blood: 0.33 mL blood+aprotinin in 0.03 mL in aliquots to obtain a final blood concentration of respectively 50 KIU mL-1; 100 KIU mL-1 and 200 KIU mL-1. The coagulation process was analysed by thromboelastography. R-time, reflecting intrinsic coagulation, increased in a dose dependent manner between NaCl-treated and aprotinin-treated blood. These findings suggest a dose dependent impairment of intrinsic coagulation by aprotinin.

The Effect of Surface Modification and Aprotinin on Cellular Injury during Simulated Cardiopulmonary Bypass

“…In addition, aprotinin can have both pro and anitcoagulative effects. Aprotinin aids in coagulation by affecting serine proteases and preserving platelet function (by reducing glycoprotein loss, while in granulocytes, it prevents the proinflammatory adhesive glycoproteins) (5,9,12,13). Although the anticoagulant mechanisms of aprotinin are relatively unknown, it is believed the activation of factor II (prothrombin) and XII (Hagemann's) are inhibited by aprotinin.…”

“…Although the anticoagulant mechanisms of aprotinin are relatively unknown, it is believed the activation of factor II (prothrombin) and XII (Hagemann's) are inhibited by aprotinin. (6,9,(12)(13)(14). The addition of aprotinin to the ECC has also been shown to inhibit fibrinolysis by reducing the release of tissue plasminogen activator (5,8,12).…”