Evaluation of the antineoplastic activity of guduchi (Tinospora cordifolia) in cultured HeLa cells

Jagetia, Ganesh Chandra; Nayak, Vijayashree; Vidyasagar, M S

doi:10.1016/s0304-3835(98)00047-0

Cited by 83 publications

(49 citation statements)

References 34 publications

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“…Previous studies have shown the pharmacological effect of T. cordifolia (Deshmukh, 1957). Antineoplastic activity of this plant has also been reported (Jagetia and Nayak, 1998). In experimental hepatic amoebiosis, a crude plant extract has been formulated (Sohni and Bhatt, 1996).…”

Section: Introductionmentioning

confidence: 95%

Hepatoprotective and immunomodulatory properties of Tinospora cordifolia in CCl4 intoxicated mature albino rats.

et al. 2002

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-Effect of Tinospora cordifolia extract on modulation of hepatoprotective and immunostimulatory functions in carbon tetrachloride (CCl 4 ) intoxicated mature rats is reported here. Administration of CCl 4 (0.7 ml/kg body weight for 7 days) produces damage in the liver as evident by estimation of enzymes such as serum glutamate oxaloacetate transaminase (SGOT), serum glutamate pyruvate transminase (SGPT) and alkaline phosphatase (ALP) as well as serum bilirubin level. CCl 4 administration also causes immunosuppressive effects as indicated by phagocytic capacity, chemotactic migration and cell adhesiveness of rat peritoneal macrophages. However, treatment with T. cordifolia extract (100 mg/kg body weight for 15 days) in CCl 4 intoxicated rats was found to protect the liver, as indicated by enzyme level in serum. A significant reduction in serum levels of SGOT, SGPT, ALP, bilirubin were observed following T. cordifolia treatment during CCl 4 intoxication. Treatment with T. cordifolia extract also deleted the immunosuppressive effect of CCl 4 , since a significant increment in the functional capacities of rat peritoneal macrophages (PMφ) was observed following T. cordifolia treatment. The results of our experiment suggest that treatment by T. cordifolia extract may be the critical remedy for the adverse effect of CCl 4 in liver function as well as immune functions.

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Section: Introductionmentioning

confidence: 95%

Hepatoprotective and immunomodulatory properties of Tinospora cordifolia in CCl4 intoxicated mature albino rats.

et al. 2002

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“…This is probably the first report describing the APE-induced DNA damage in HeLa cells. Similarly, doxorubicin, extract of Tinospora cordifolia, acyclovir and azidothymidine have been reported to increase the frequency of micronuclei in a concentration-dependent manner in HeLa cells earlier [8][9][10]35]. The treatment of V79 cells with doxorubicin has been found to increase the micronuclei frequency in concentration dependent manner [11].…”

Section: Discussionmentioning

confidence: 99%

“…Cisplatin has also been reported to induce micronuclei in 9L tumor cells [7]. Similarly, adriamycin, acyclovir or methanolic extract of Tinospora cordifolia were reported to induce micronuclei in HeLa and V79; cells [8][9][10][11]. The use of natural products, especially plants may be beneficial in cancer treatment as more than 80% of the world population still depend on plants and natural products for healthcare [12,13].…”

Section: Introductionmentioning

confidence: 99%

Determination of Antineoplastic Activity of Rohituka, Aphanamixis Polystachya (Wall) RN Parker in Hela Cells: Correlation with Clonogenicity and DNA Damage

Jagetia

Venkatesha

2016

IJCAM

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The antineoplastic activity of chloroform stem bark extract of rohituka, Aphanamixis polystachya (APE) used traditionally to treat spleen and liver tumors was evaluated in cultured HeLa cells by clonogenic and micronucleus assays. Treatment of HeLa cells with 0, 5, 10, 25, 50, 75 or 100µg/ml APE for different times caused a concentration-dependent reduction in the cell survival up to 6 h post-treatment (p<0.005) followed by a non-significant decline in the cells treated with APE for 24 h. Therefore, 6h treatment time was considered as an optimum time for APE exposure and further studies were carried out using this treatment time. Exposure of HeLa cells to different concentrations of APE resulted in a concentration-dependent decline in the cell viability and 100µg/ ml APE resulted in 72% cell death when compared with the non-drug treated control group. These results were further confirmed by clonogenic assay where, APE treatment caused a concentration-dependent decline in the clonogenicity of HeLa cells, and the surviving fraction of cells was reduced to 0.22 after treatment with 100µg/ml APE, the highest concentration screened. The inhibitory concentration (IC50) of APE was found to be 25µg/ml. The APE-induced DNA damage was estimated by micronucleus assay, where the treatment of HeLa cells with various concentrations of APE resulted in a concentration-dependent rise in the frequency of micronuclei in binucleate HeLa cells (MNBNCs) at 20, 30, and 40 h post-treatment. This increase in MNBNCs was significantly higher than the baseline frequency of MNBNCs. APE treatment also increased the frequency of HeLa cells bearing more than one micronucleus (MN) indicating higher degree of DNA damage. The frequency of MNBNC increased with scoring time and a maximum elevation in the MNBNC frequency was observed at 30 h posttreatment. The MNBNC induction and clonogenicity of HeLa cells was inversely related indicating that increased MNBNC formation resulted in a corresponding reduction in the cell survival. The cell proliferation indices (PI) declined with increasing concentration of APE that increased with screening time. Our study demonstrates that APE is able to kill HeLa cells effectively and this cell killing effect of APE may be due its ability to induce DNA damage.

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“…15,16 Various in vitro and in vivo studies on protoberberine alkaloids, especially berberine, palmatine, and jatrorrhizine, have suggested their potential as adjuvants in chemotherapy. 17 Other botanicals rich with these alkaloids include Berberis aristata, Berberis wilsoniae, and Thalictrum javanicum.…”

Section: Introductionmentioning

confidence: 99%

Effect of Botanical Immunomodulators on Human CYP3A4 Inhibition

et al. 2013

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Purpose. Many botanical immunomodulators are used as adjuvants along with cancer chemotherapy. However, information on the impact of concurrent administration of such botanicals on pharmacokinetics of chemotherapy agents is inadequate. This study investigates inhibitory activities of 3 popular botanical adjuvants: Asparagus racemosus (root aqueous extract; ARE), Withania somnifera (root aqueous extract; WSE), and Tinospora cordifolia (stem aqueous extract, TCE) on human CYP3A4 isoenzyme, responsible for metabolism of several chemotherapy agents. Experimental design. Testosterone 6-β hydroxylation was monitored using high-performance liquid chromatography as an indicator of CYP3A4 catalytic activities. Ketoconazole (positive control) and extracts were studied at their in vivo-relevant concentrations. Results. TCE showed mild inhibition while no significant inhibitory activities were observed in WSE and ARE. TCE was further fractionated to obtain polar and nonpolar fractions. The nonpolar fraction showed significant CYP3A4 inhibition with IC 50 13.06 ± 1.38 µg/mL. Major constituents of nonpolar fraction were identified using HPLC-DAD-MS profiling as berberine, jatrorrhizine, and palmatine, which showed IC 50 values as 6.25 ± 0.30, 15.18 ± 1.59, and 15.53 ± 1.89 µg/mL, respectively. Conclusion. Our findings suggest that constituents of TCE extract especially protoberberine alkaloids have the potential to interact with cancer chemotherapy agents that are metabolized by CYP3A4 in vivo.

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Evaluation of the antineoplastic activity of guduchi (Tinospora cordifolia) in cultured HeLa cells

Cited by 83 publications

References 34 publications

Hepatoprotective and immunomodulatory properties of Tinospora cordifolia in CCl4 intoxicated mature albino rats.

Hepatoprotective and immunomodulatory properties of Tinospora cordifolia in CCl4 intoxicated mature albino rats.

Determination of Antineoplastic Activity of Rohituka, Aphanamixis Polystachya (Wall) RN Parker in Hela Cells: Correlation with Clonogenicity and DNA Damage

Effect of Botanical Immunomodulators on Human CYP3A4 Inhibition

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