Evaluation of the Antioxidant Activities and Tyrosinase Inhibitory Property from Mycelium Culture Extracts

Abstract

Since mushrooms have many bioactive components, they have been used as components in folk medicine. Because mycelium has an advantage when it comes to large-scale production, this study aimed to evaluate the antioxidant properties and anti-tyrosinase activity from 55 mycelia in culture media. Relatively high 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging capacity was detected from the ethanol extract of culture media including mycelium (EECiM) of Morchella esculenta var. esculenta (MEVE), Auricularia polytricha (APO), Tremella aurantia (TAU), Volvariella bombycina (VBO), and Oudemansiella sp. (Osp), which also showed strong reducing power and inhibitory activity in relation to the thiobarbituric acid (TBA) value. On the other hand, relatively high tyrosinase inhibitory activity was detected in Inonotus mikadoi (IMI), Coriolus versicolor (CVE), Volvariella volvacea (VVO), Panellus serotinus (PSE), Auricularia auricula (AAU), and Fomitopsis sp. (Fsp). Interestingly, the APO EECiM exhibited the highest DPPH radical scavenging rate (77.5 ± 4.3%) and reducing power (1.18 ± 0.041), while the highest inhibitory power of the TBA value and antityrosinase activity were detected in that of TAU (64.5 ± 4.1%) and IMI (46.0 ± 7.5%), respectively. Overall, our study suggested potential candidates for EECiMs that exhibited powerful antioxidant and tyrosinase inhibitory properties and might be used as natural antioxidant tyrosinase inhibitor.

Mushrooms extracts and compounds in cosmetics, cosmeceuticals and nutricosmetics—A review

“…Although Pleurotus species are the most studied mushroom species with reported anti-tyrosinase potential, none of those studies were able to identify the bioactive metabolites responsible for this activity. Park et al (2015b) recently studied the tyrosinase inhibitory activity of several ethanolic mycelium extracts in the presence of arbutin and ascorbic acid as positive controls. Among the studied extracts, Inonotus mikadoi (Lloyd) Gilb.…”

An Edible Mushroom With Medicinal Significance; Auricularia polytricha

“…In these studies, both ethanol and water extracts of A. polytricha have significant antioxidant activities determined using different methods such as in vitro free radical scavenging assays, DPPH assay and TAS assay [29][30]. In a previously reported work, A. polytricha had high DPPH scavenging activity [31]. Another study showed that A. polytricha, which is among the edible mushrooms, had the strongest radical scavenging and metal chelating activities in addition to the highest polyphenolic and flavonoid contents [32].…”

Effects of element complexes containing Fe, Zn and Mn on artificial morel’s biological characteristics and soil bacterial community structures

“…Morchella spp (morel) is highly prized for its medicinal and nutritional qualities, such as antioxidant, anti-inflammatory and antitumor activities, as well as strengthening the immune response [1~4]. According to previous experiments, morel mycelia growth and fruit-body (ascocarp) formation are strongly affected by many factors, such as temperature, humidity, illumination, air, pH and nutrition.…”