Evaluation of the Antiproliferative Activity of the Leaves from Arctium lappa by a Bioassay-Guided Fractionation

Machado, Fábio Bahls; Yamamoto, R.; Zanoli, Karine; Nocchi, Samara Requena; Novello, Cláudio Roberto; Schuquel, Ivânia T.A.; Sakuragui, Cássia Mônica; Luftmann, Heinrich; Ueda-Nakamura, Tânia; Nakamura, Celso Vataru; Mello, João Carlos Palazzo de

doi:10.3390/molecules17021852

Cited by 46 publications

(31 citation statements)

References 18 publications

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“…The study was inspired by the fact that the greater burdock (Arctium lappa L.) belonging to the Asteraceae family, found throughout the world, exhibits antitumor activity against a number of cell lines (Machado et al 2012). Powdered roots of common sowthistle (Sonchus oleraceus L.) growing in Egypt proved to be another source of loliolide (1).…”

Section: Compoundmentioning

confidence: 99%

Loliolide - the most ubiquitous lactone

et al. 2015

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The searching for biologically active compounds produced by living organisms led to the discovery of a number of compounds with more or less complicated structure. One of the simplest molecules are monoterpenoid lactones and loliolide is the most common among them. Loliolide was found in animals (insects) and plants (flowers, shrubs, trees) both terrestrial and marine, such as algae and corals. Many years of research on plants used in traditional folk medicine of different countries have led to the conclusion that this compound has a variety of biological properties such as anti-cancer, antibacterial, antifungal and antioxidant ones. Moreover, plants containing loliolide are used in alternative medicine in treatment of diabetes and depression. It is extremely interesting that this lactone also affects the behavior of ants as well as the development of certain plants (allelopathic activity). However, sometimes there are side effects as in the case of structural analogues of loliolide contributing to extinction of tropical coral.

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Section: Compoundmentioning

confidence: 99%

Loliolide - the most ubiquitous lactone

et al. 2015

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“…Although burdock leaves have been reported to possess antioxidant and antiproliferative activities (26)(27)(28), little is known about their inhibitory effect on skin aging-associated enzymes. Therefore, the present study investigated the potential inhibitory effect of burdock leaves on elastase and tyrosinase activity.…”

Section: Introductionmentioning

confidence: 99%

Inhibitory effect of burdock leaves on elastase and tyrosinase activity

Horng

Chiang

et al. 2017

Experimental and Therapeutic Medicine

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Abstract. Burdock (Arctium lappa L.) leaves generate a considerable amount of waste following burdock root harvest in Taiwan. To increase the use of burdock leaves, the present study investigated the optimal methods for producing burdock leaf extract (BLE) with high antioxidant polyphenolic content, including drying methods and solvent extraction concentration. In addition, the elastase and tyrosinase inhibitory activity of BLE was examined. Burdock leaves were dried by four methods: Shadow drying, oven drying, sun drying and freeze-drying. The extract solution was then subjected to total polyphenol content analysis and the method that produced BLE with the highest amount of total antioxidant components was taken forward for further analysis. The 1,1-diphenyl-2-pycrylhydrazyl scavenging, antielastase and antityrosinase activity of the BLE were measured to enable the evaluation of the antioxidant and skin aging-associated enzyme inhibitory activities of BLE. The results indicated that the total polyphenolic content following extraction with ethanol (EtOH) was highest using the freeze-drying method, followed by the oven drying, shadow drying and sun drying methods. BLE yielded a higher polyphenol content and stronger antioxidant activity as the ratio of the aqueous content of the extraction solvent used increased. BLE possesses marked tyrosinase and elastase inhibitory activities, with its antielastase activity notably stronger compared with its antityrosinase activity. These results indicate that the concentration of the extraction solvent was associated with the antioxidant and skin aging-associated enzyme inhibitory activity of BLE. The reactive oxygen species scavenging theory of skin aging may explain the tyrosinase and elastase inhibitory activity of BLE.In conclusion, the optimal method for obtaining BLE with a high antioxidant polyphenolic content was freeze-drying followed by 30-50% EtOH extraction. In addition, the antielastase and antityrosinase activities of the BLE produced may be aid in the development of skincare products with antiwrinkle and skin-evening properties. IntroductionThe skin is the largest organ of the human body and has essential functions, including protecting internal organs and regulating body temperature. Collagen and elastin, which are naturally occurring structural proteins in the dermis of the skin, maintain the plumpness, flexibility and elasticity of the skin. Skin aging presents as wrinkling, roughness, dryness, decreased elasticity and uneven pigmentation (1,2). In addition to intrinsic aging, extrinsic aging caused by exposure of the skin to solar radiation is an important concern. Reactive oxygen species (ROS) caused by ultraviolet (UV) radiation are clinically associated with specific markers of photoaging, including wrinkling, and increased elastin and collagen damage (2,3). Elastase digests elastin (4,5). Pigmentary changes due to the accumulation of melanin are another major characteristic of photoaging. Tyrosinase is a key enzyme that catalyzes melanin biosynthesis in...

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“…Antiinflammatory, gastroprotective and antioxidant activities have been reported for root extracts [8,9,10,11], anticancer, antidiabetic and anti-inflammatory activities for seed extracts [12,13,14], antiviral and antidiabetic activities for fruit extracts [15,16], and antimicrobial and anticancer activities for leaves extracts [17,18].…”

Section: Introductionmentioning

confidence: 99%