Evaluation of the Antityrosinase and Antioxidant Potential of Zinc Oxide Nanoparticles Synthesized From the Brown Seaweed-Turbinaria Conoides

Abstract

Objective: The present study was designed to evaluate the antioxidant and antityrosinase properties of zinc oxide nanoparticles (ZnO-NPs) synthesized from brown seaweed Turbinaria conoides.Methods: Zinc Oxide Nanoparticles were synthesized from the hydroethanolic extract of Turbinaria conoides. Ultraviolet-Visible Spectrophotometric analysis was performed to confirm the formation of ZnO-NPs. Size, morphology and elemental composition of ZnO-NPs were analysed using SEM-EDAX. The antioxidant activity of the synthesized zinc oxide nanoparticles was investigated by total antioxidant capacity (phosphomolybdenum method), reducing power assay and ferric reducing antioxidant power assay (FRAP). Anti tyrosinase activity was assessed to validate the skin whitening ability of the ZnO-NPs. Results:The antioxidant activity of ZnO-NPs synthesized from hydroethanolic extract of Turbinaria conoides was maximum when compared with that of the hydroethanolic algal extract. The antityrosinase activity of ZnO-NPs was found to be maximum with 75% tyrosinase inhibition when compared to hydroethanolic algal extract which had 56% inhibition at 250μg/ml concentration. Conclusion:Overall our study provides a firm evidence to support that antityrosinase and antioxidant activities are exhibited by ZnO-NPs synthesized from hydroethanolic extract of Turbinaria conoides and it might be used as an antioxidant and as a source of skin whitening agent in cosmetics.

Hydrothermal Assisted Synthesis and Biological Evaluation of Biogenic Silver Nanoparticles

“…The results of the tyrosinase inhibitory screening in this study for AgNPsarebetter than those obtained from tyrosinase inhibitory screening for AgNPs and ZnONPsfrom extracts of Artemisia annua and seaweed-turbinaria conoides, which gave 40% (100mg/mL) and 50% (100μg/mL) respectively [13,14]. The results of tyrosinase inhibitory activity of AgNPs (20.83 μg/mL) was also better than those we had reported for ZnO-NPs (obtained from aqueous leaf extracts of A.laxiflora) with IC50 of66.28μg/mL [14] and AgNPs (obtained from aqueous leaf extracts of Euphorbia sanguine) with IC50 of 71.96µg/ml [34].The improved tyrosinase inhibition prompted further investigation of the kinetics of the inhibition and mode of inhibitor-tyrosinase interaction.…”

“…Biogenic silver obtained from extracts of Hovenia dulcis fruit extract [13] and zinc oxide nanoparticles obtained from brown seaweed of Turbinaria conoides [14] have been reported to exhibit tyrosinase inhibition. Tyrosinase inhibition is an efficient mechanism in the treatment of skin diseases of hyperpigmentation such as melasma, freckles, age spots and senile lentigo [15].…”

“…The plant is used traditionally in the treatment of many diseases such as pile, Dysentery, Malaria, Eczema, Cough and High fever [19]. The reducing and stabilizing potentials of the phytochemicals of Alchornea laxiflora have been exploited in plant mediated synthesis of various nanoparticles such as zinc oxide [14] and copper [20]. The biogenic copper nanoparticle was reported to show high catalytic activity in the oxidative desulphurization of model oil.…”

Protective effects of zinc oxide nanoparticles against doxorubicin induced testicular toxicity and DNA damage in male rats

“…23 Moreover, Dani and Dhawan 24 and Malekirad et al 25 reported that Zn is a protecting antioxidant due to its ability to bring the malondialdehyde (MDA) levels to near normal levels. Additionally, Raajshreer and Durairaj 26 revealed that their synthesized ZnO NPs possess antioxidant activity with respect to mechanisms of both reducing activities and free radical scavenging.…”