Evaluation of the applicability of amplified rDNA-restriction analysis (ARDRA) to identification of species of the genus Corynebacterium

Vaneechoutte, Mario; Riegel, Philippe; Briel, D. De; Monteil, H.; Verschraegen, Gerda; Rouck, Ann De; Claeys, Geert

doi:10.1016/0923-2508(96)81061-8

Cited by 54 publications

(35 citation statements)

References 21 publications

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“…Estes avanços na taxonomia e métodos de detecção, junto com o interesse nas corinebactérias como agentes oportunistas em infecções em humanos, recentemente resultou no delineamento de um vasto grupo de novas espécies deste gênero (Khamis et al 2005). A grande maioria das espécies de Corynebacterium pode ser diferenciada pelo polimorfismo das bandas de restrição para o gene do RNAr 16S devido à alta variabilidade das seqüências do DNAr 16S interespécies para esse gênero, que pode ser visto por um grande número de padrões de restrição (Vaneechoutte et al 1995).…”

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Comparação genotípica de isolados de Corynebacterium pseudotuberculosis de caprinos e ovinos do sertão de Pernambuco

Abreu¹,

Mota

Rosinha

et al. 2008

Pesq. Vet. Bras.

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Objetivou-se com este estudo comparar genotipicamente 35 isolados de Corynebacterium pseudotuberculosis recuperados de conteúdo de abscessos de caprinos e ovinos com linfadenite caseosa, procedentes de cinco municípios localizados no Sertão de Pernambuco, Brasil. Utilizou-se a técnica de fingerprint RFLP-PCR com as enzimas de restrição Hpy-Ch4 e Msp1 aplicada ao gene rpoB e as enzimas Pst I e Msp I para o gene pld. Não houve diferença nos padrões de fragmentos de bandas entre os isolados, independente da espécie hospedeira ou da área geográfica estudada, definindo-se um padrão genotípico homogêneo de C. pseudotuberculosis responsável por abscessos superficiais na região.

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Comparação genotípica de isolados de Corynebacterium pseudotuberculosis de caprinos e ovinos do sertão de Pernambuco

Abreu¹,

Mota

Rosinha

et al. 2008

Pesq. Vet. Bras.

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“…ARDRA has been studied most thoroughly and has been used for the identification of species of different taxa, e.g. Acinetobacter (39), Clostridium (37), Comamonadaceae (4 l), Corynebacterium (40), Mollicutes (9), Mycobacterium (38), Leptospira (29) and Streptococcus (1 9), and to study phylogenetic relationships in Bacillus (16), Clostridium (13), Moraxella (18) and Xanthomonas (28).…”

Section: Introductionmentioning

confidence: 99%

Comparison of PCR-based DNA fingerprinting techniques for the identification of Listeria species and their use for atypical Listeria isolates

Vaneechoutte¹,

Tichy²,

Bannerman³

et al. 1998

International Journal of Systematic Bacteriology

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Four PCR-based DNA fingerprinting techniques were compared for their ability to identify a t the species level a heterogeneous collection of isolates belonging to the six valid Listeria species. 16s rDNA-RFLP analysis identified all species and 16s rDNA-SSCP analysis identified almost all species. Also, isolates with unusual biochemical characteristics and/or unusual antigenic composition could be identified correctly. rRNA-intracistronic length polymorphism analysis suffered from high intraspecific variability, a limited number of fragments per profile, and small length differences between the spacers of different species. tRNA-intergenic length polymorphism analysis resulted in identification of all isolates but one, when fluorescent DNA capillary electrophoresis was used such that fragment length differences of 1 bp could be resolved. The four techniques yielded comparable results relevant to the taxonomy of Listeria. They all indicate a high degree of genetic relatedness between L. innocua and L. welshimeri, homogeneity of L. grayi, distinct but clear relatedness of L. grayi to the other Listeria species, a clear distinction between the two subspecies of L. ivanovii, and a clear distinction between Listeria isolates and isolates from closely related taxa or from species which are phenotypically difficult to distinguish from Listeria. New sequence determination of the 16s rRNA gene was necessary to obtain sequences in accordance with the findings of 165 rDNA-RFLP analysis.

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“…BioTechniques 23:584-588 (October 1997) Polymerase chain reaction (PCR)-based assays have been developed to identify microscopic organisms that cause disease (8) or have an environmental impact (1). In both cases, DNA is extracted from pooled individuals.…”

Section: Preparation Of Dna From Numerous Individual Microscopic Orgamentioning

confidence: 99%

“…In one of our laboratories, amplification of the 16S rRNA gene, followed by restriction analysis of the product, is being developed to identify unknown bacteria to species level (8). Using PCR primers annealing to well-conserved sequences at both termini of the bacterial 16S rRNA gene allows for a precise differentiation to species level by direct or indirect determination of the nucleotide sequence within the products.…”

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Long-Term Storage of Unamplified Complete PCR Mixtures

et al. 1997

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Evaluation of the applicability of amplified rDNA-restriction analysis (ARDRA) to identification of species of the genus Corynebacterium

Cited by 54 publications

References 21 publications

Comparação genotípica de isolados de Corynebacterium pseudotuberculosis de caprinos e ovinos do sertão de Pernambuco

Comparação genotípica de isolados de Corynebacterium pseudotuberculosis de caprinos e ovinos do sertão de Pernambuco

Comparison of PCR-based DNA fingerprinting techniques for the identification of Listeria species and their use for atypical Listeria isolates

Long-Term Storage of Unamplified Complete PCR Mixtures

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