Evaluation of the autologous bone marrow mononuclear therapy and functional restoration in the scarred myocardium by imaging analysis

Gokhale, Alla Gopala Krishna; Chelluri, Lakshmi Kiran; Kumaresan, K; Subramanyam, Guru; Sudhakar, K.; GPV, Subbaiah; Debnath, Tanya; Ks, Ratnakar

doi:10.4103/0975-3583.83037

Cited by 9 publications

(7 citation statements)

References 15 publications

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“…The phenotype of human ADSCs is quite similar to BMSCs 23 . The stages of the cell cycle in proliferating human ADSCs were studied at different timepoints.…”

Section: Discussionmentioning

confidence: 99%

Standardization and quality assessment for clinical grade mesenchymal stem cells from human adipose tissue

Debnath¹,

Chelluri

2019

Hematology, Transfusion and Cell Therapy

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BackgroundMesenchymal stem cells have immense potential in stem cell-based therapies, however there is a pre-requisite to develop a curative cell dose. Adipose tissue-derived mesenchymal stem cells are promising mainly due to their potential abundance, immunomodulatory effect and remarkable differentiation potential. Nevertheless, senescence may develop during their in vitro expansion due to the incidence of genetic instability. Hence, it is important to attain an ideal balance between mesenchymal stem cell growth, quality and genetic integrity before their clinical use.MethodsStromal vascular fraction was obtained from omentum tissue of patients undergoing liposuction procedures for morbid obesity. This study standardized a closed system protocol which can be utilized for clinical grade stem cell derivation. Stages of cell growth and characterization of human adipose tissue-derived mesenchymal stem cells were also assessed along with the chromosomal stability in these in vitro cultures.ResultsHuman adipose tissue-derived mesenchymal stem cells maintained their spindle-shaped morphology and were able to proliferate and renew, confirming their suitability for in vitro cultivation and generate clinical grade mesenchymal stem cells. Immunophenotyping indicates that the cells expressed cluster of differentiation (CD)73/CD90/CD105, mesenchymal stem-cell markers, while lacked CD34/CD45/ Human Leukocyte antigen-antigen D related (HLA-DR) expression (hematopoietic cell markers). A cell cycle study demonstrated growth kinetics under in vitro culture conditions. Human adipose tissue derived mesenchymal stem cells expressed normal cell karyotype by chromosomal G-banding indicating their genetic stability at Passage 5. Mesenchymal stem cells also demonstrated trilineage differentiation.ConclusionsAvailability of adipose tissue in abundance is a major advantage for clinical applications. Furthermore, detailed characterization of human adipose tissue-derived mesenchymal stem cells, their genomic stability and differentiation potential from stromal vascular fraction of human adipose tissue would help assist in tissue regeneration and repair.

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“…The phenotype of human ADSCs is quite similar to BMSCs 23 . The stages of the cell cycle in proliferating human ADSCs were studied at different timepoints.…”

Section: Discussionmentioning

confidence: 99%

Standardization and quality assessment for clinical grade mesenchymal stem cells from human adipose tissue

Debnath¹,

Chelluri

2019

Hematology, Transfusion and Cell Therapy

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“…This strategy, although rational, has not allowed the detection of cell mechanisms of action that are independent of revascularization. While the reported benefits can thus be attributed to a potential synergy 13 between the favorable environment created by the revascularization of the region and the MSCs, the precise delineation of each contribution, however, remains unknown.…”

Section: Introductionmentioning

confidence: 99%

Autologous Mesenchymal Stem Cells Produce Concordant Improvements in Regional Function, Tissue Perfusion, and Fibrotic Burden When Administered to Patients Undergoing Coronary Artery Bypass Grafting

Karantalis

DiFede

Gerstenblith

et al. 2014

Circulation Research

320

235

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Rationale While accumulating data support the efficacy of intramyocardial cell-based therapy to improve LV function in patients with chronic ischemic cardiomyopathy undergoing CABG, the underlying mechanism and impact of cell injection site remain controversial.Mesenchymal stem cells (MSCs) improve LV structure and function through several effects including: reducing fibrosis, neoangiogenesis and neomyogenesis. Objective To test the hypothesis that the impact on cardiac structure and function following intramyocardial injections of autologous MSCs results from a concordance of pro-recovery phenotypic effects. Methods and Results Six patients were injected with autologous MSCs into akinetic/hypokinetic myocardial territories not receiving bypass graft for clinical reasons. MRI was used to measure scar, perfusion, wall thickness and contractility at baseline, 3, 6 and 18 months and to compare structural and functional recovery in regions that received MSC injections alone, revascularization alone, or neither. A composite score of MRI variables was used to assess concordance of antifibrotic effects, perfusion, and contraction at different regions. After 18 months, subjects receiving MSCs exhibited increased LVEF (+9.4±1.7%, p=0.0002) and decreased scar mass (-47.5±8.1%; p<0.0001) compared to baseline. MSC-injected segments had concordant reduction in scar size, perfusion and contractile improvement (concordant score: 2.93±0.07), whereas revascularized (0.5±0.21) and non-treated segments (-0.07±0.34) demonstrated non-concordant changes (p<0.0001 vs. injected segments). Conclusions Intramyocardial injection of autologous MSCs into akinetic yet non-revascularized segments produces comprehensive regional functional restitution, which in turn drives improvement in global LV function. These findings, although inconclusive due to lack of placebo group, have important therapeutic and mechanistic hypothesis-generating implications.

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“…The proportion of bone marrow and ficoll was 1:3; centrifugation was performed for 30 min at 1500 RPM. The cells were counted following which the viability was determined using the trypan blue dye exclusion method, mononuclear cells were cultivated for 24 h in the DMEM medium supplemented with 10% fetal bovine serum, 1% penicillin, and streptomycin in a humidified incubator (Steri Cycle I 160, Thermo) maintained at 37 • C and 5% CO 2 according to our published literature [36].…”

Section: Human Bone Marrow Stromal Cells (Hbmscs) Isolation and Culturementioning

confidence: 99%

Inorganic nanorods direct neuronal differentiation of bone marrow-derived mesenchymal stem cells

et al. 2023

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Mesenchymal stem cells (MSCs) are multipotent cells and are considered a potential source for tissue and organ repair due to their self-renewal, proliferation, and differentiation abilities. However, in most cases, MSCs are needed to be stimulated with external growth factors to promote their proliferation and differentiation. Over the past decade, it has been demonstrated that nanomaterials could facilitate MSC proliferation and differentiation, and excellent efforts are carried out to investigate their possible modulating pattern and mechanisms for MSC differentiation. Europium hydroxide (EuIII(OH)3) nanorods (EHN) are well-researched for their biomimicking properties and act as a substitute for growth factors that induce cell proliferation, migration, and differentiation. In the current study, the human mesenchymal stem cells (MSCs) were chosen as an in vitro model for evaluating the role of EHN in modulating the differentiation process of MSCs into neuronal and glial lineages. The characterization of MSCs and differentiated neuronal cells observed by flow cytometry, confocal, and gene marker expression studies supported our hypothesis that the EHNs are pro-angiogenic and pro-neurogenic. Finally, altogether our results suggest that EHNs have the potential to play an essential part in developing novel treatment strategies for neurodegenerative diseases (ND) and spinal cord injuries (SCI) based on the nanomedicine approach.

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Evaluation of the autologous bone marrow mononuclear therapy and functional restoration in the scarred myocardium by imaging analysis

Cited by 9 publications

References 15 publications

Standardization and quality assessment for clinical grade mesenchymal stem cells from human adipose tissue

Standardization and quality assessment for clinical grade mesenchymal stem cells from human adipose tissue

Autologous Mesenchymal Stem Cells Produce Concordant Improvements in Regional Function, Tissue Perfusion, and Fibrotic Burden When Administered to Patients Undergoing Coronary Artery Bypass Grafting

Inorganic nanorods direct neuronal differentiation of bone marrow-derived mesenchymal stem cells

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