Evaluation of the BacT/ALERT 3D system for recovery and drug susceptibility testing of Mycobacterium tuberculosis

Ängeby, Kristian; Werngren, Jim; Toro, Juan Carlos; Hedström, G.; Petrini, Bj⊘rn; Hoffner, Sven

doi:10.1046/j.1469-0691.2003.00783.x

Cited by 46 publications

(30 citation statements)

References 8 publications

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“…Each method has its own advantages and disadvantages, starting from the LJ medium and the Middlebrook 7H 10 medium (MB7H10) to the present trendy and speedy automated methods like the MB/BACT 3D device [8][9][10][11][12][13]. MB/BACT is a safer and a quicker method as it is an automated method which involves liquid media and as it does not involve any radioactive material.…”

Section: Resultsmentioning

confidence: 99%

Comparison of the Lowenstein-Jensen Medium, the Middlebrook 7H10 Medium and MB/BacT for the Isolation of Mycobacterium Tuberculosis (MTB) from Clinical Specimens

Naveen¹,

Peerapur²

2012

JCDR

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Introduction: Tuberculosis (TB) is the most common cause of death due to a single infectious agent worldwide in adults. India alone accounts for 30% of the global tuberculosis burden. There is a need for a method of cultivation of mycobacteria that is reliable and economical and has a short turnaround time.Objective: The present study was attempted to assess the feasibility of using MB BACT and Middlebrook 7h10(MB7H10) as primary isolation media for mycobacteria. They were compared with the LJ medium, which was the gold standard. Materials and Methods:Various clinical specimens from a total of 236 clinically suspected cases of TB were studied. All the samples were decontaminated by using the modified Petroff's method. Each sample was subjected to ZN staining and it was simultaneously inoculated onto the LJ medium, the MB7H10 medium and MB BACT. The growth from the cultures were confirmed by ZN staining and they were speciated by using biochemical reactions.Results: Out of the 236 samples which were screened, 116 isolates were obtained. All the 116 were isolated from MB BACT, 82 were isolated from the LJ medium and 62 were isolated from MB7H10. 82 isolates were obtained from MB BACT and the LJ medium, 62 were obtained from MB7H10 and MB BACT, 58 were isolated from LJ and MB7H10 and 58 were isolated from LJ medium, MB7H10 and MB bact. Neither the L J medium nor the Middlebrook 7h 10 medium could isolate mycobacteria exclusively. It showed that the combination of media did not prove to be superior over the use of MB BACT alone. The average isolation time of L J, the MB7H10 medium and MB BACT was 30

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Section: Resultsmentioning

confidence: 99%

Comparison of the Lowenstein-Jensen Medium, the Middlebrook 7H10 Medium and MB/BacT for the Isolation of Mycobacterium Tuberculosis (MTB) from Clinical Specimens

Naveen¹,

Peerapur²

2012

JCDR

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“…), BacT/ ALERT 3D (bioMérieux, Durham, NC), or ESP Culture System II (Trek Diagnostics, Inc., Westlake, Ohio) to perform indirect susceptibility tests (which require a pure culture of Mycobacterium tuberculosis), the results are available anywhere after 9 to 30 days. If susceptibility tests are performed directly from clinical specimens with these systems, the time needed for results is between 4 and 23 days (2,3,6,15,18). Although rapid, these methods require expensive substrates and equipment and are therefore not feasible in most developing countries.…”

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confidence: 99%

Drug Susceptibility Testing of Mycobacterium tuberculosis by a Nitrate Reductase Assay Applied Directly on Microscopy-Positive Sputum Samples

et al. 2005

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Current methods for drug susceptibility testing of Mycobacterium tuberculosis are either costly or slow. As the prevalence of multidrug-resistant strains increases, the need for fast, reliable, and inexpensive methods that can also be applied in settings with scarce resources is obvious. We evaluated a rapid colorimetric nitrate reductase assay (NRA) for direct drug susceptibility testing of M. tuberculosis directly from clinical sputum samples with positive microscopy results for acid-fast bacilli with more than 10 acid-fast bacilli per high-power field. We have saved valuable time by omitting the preisolation step. The sensitivity (ability to detect true drug resistance) and specificity (ability to detect true drug susceptibility) of the direct NRA, using the direct proportion method as the reference, were 100 and 100%, 93 and 100%, 76 and 100%, and 55 and 99% for rifampin, isoniazid, streptomycin, and ethambutol, respectively, when tested on M. tuberculosis strains present in 121 samples. The results were in most cases available in 14 days. The direct NRA could be used as a rapid, inexpensive, and accurate method to determine rifampin and isoniazid susceptibility directly from sputum. The technique might become a valid alternative to traditional methods, especially in low-income countries.There is no doubt that the global tuberculosis epidemic remains a problem for public health, and there is also an emergence of multidrug-resistant tuberculosis that is worsening the impact of this disease (10,19). In order to fight this situation, we need a rapid and inexpensive drug susceptibility test to allow a prompt initiation of correct antibiotic therapy.With traditional methods, such as the indirect or direct proportion method, it could take 3 to 4 weeks to obtain susceptibility results (4, 8). The time needed to obtain these results represents a potential danger to patients, health workers, and the community (11, 16). Using liquid medium-based culture systems such as the BACTEC 460 TB system (Becton Dickinson, Sparks, Md.), the Mycobacteria Growth Indicator Tube MGIT 960 (Becton Dickinson, Cockeysville, Md.), BacT/ ALERT 3D (bioMérieux, Durham, NC), or ESP Culture System II (Trek Diagnostics, Inc., Westlake, Ohio) to perform indirect susceptibility tests (which require a pure culture of Mycobacterium tuberculosis), the results are available anywhere after 9 to 30 days. If susceptibility tests are performed directly from clinical specimens with these systems, the time needed for results is between 4 and 23 days (2,3,6,15,18). Although rapid, these methods require expensive substrates and equipment and are therefore not feasible in most developing countries. A recent article (1) described a rapid and inexpensive nitrate reductase assay (NRA) for the drug susceptibility testing (DST) of M. tuberculosis. This technique is based on the property of M. tuberculosis to reduce nitrate to nitrite, which is revealed as a color change of the culture media, using the Griess method (1, 7). With the NRA, it was possible to obtain ...

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“…Rapid liquid culture-based techniques have been established that can detect growth dependent changes such as CO2 production (BACTEC460 and MB/BacT) or oxygen consumption (MGIT and VersaTREK). [8][9][10][11] VersaTREK TM (formerly ESP culture system II) is an automated non-radiometric DST method. Very few studies are available on the performance of VersaTREK TM .…”

Section: Discussionmentioning

confidence: 99%

A study on pattern of initial and acquired drug resistance for isoniazid and rifampicin in A.F.B. positive sputum smears of pulmonary tuberculosis patients at a Medical College in North Eastern Uttar Pradesh, India

Tiwari

Haider

Shukla

et al. 2017

Int J Basic Clin Pharmacol

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Background: Despite availability of good quality anti-tubercular drugs and its administration through Directly Observed Therapy Short Course (DOTS) strategy of Revised National Tuberculosis Control Programme (RNTCP), tuberculosis remains a major cause of morbidity and mortality in India. The emergence of drug resistance necessitates the timely detection of susceptibility of anti-TB drugs. This can help in appropriate modification in treatment strategies.Methods: A total of 50 patients of pulmonary TB with AFB positive sputum smears attending the OPD of TB and Chest department of B.R.D. Medical College, Gorakhpur were included. Patients were grouped based on history into new (cat-I) and previously treated patients (cat-II). Cat-II patients were further subdivided into defaulter, treatment failure and relapse groups. The culture and DST of AFB positive sputum smears of these patients was done in VersaTREK™®. At the end of study, patients were grouped according to age, sex, category and drug sensitivity pattern for Isoniazid (INH) and Rifampicin (RIF) viz mono resistance (resistance to either INH or RIF) or multi drug resistance (M.D.R.) and the resultant data were analysed.Results: Of the total 50 patients included in this study, 18 (36%) patients were sensitive to both the drugs INH and RIF, of which 11 (22%) were of cat-I and 7 (14%) of category-II. Twenty-two (44%) patients were resistant to INH only of which 8 (16%) were of cat-I and 14 (28%) of cat-II. One (2%) case of cat-I showed resistance to RIF only, while M.D.R. type of resistance is seen in 1 (2%) patient of cat-I and 8 (16%) patients of cat-II. Pattern of resistance to both INH and RIF together (i.e. M.D.R. type) showed significant difference between cat-I and cat-II.Conclusions: Most of the patients showing resistance to INH, RIF or both INH and RIF (M.D.R.) belonged to category-II (previously treated) patients.

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Evaluation of the BacT/ALERT 3D system for recovery and drug susceptibility testing of Mycobacterium tuberculosis

Cited by 46 publications

References 8 publications

Comparison of the Lowenstein-Jensen Medium, the Middlebrook 7H10 Medium and MB/BacT for the Isolation of Mycobacterium Tuberculosis (MTB) from Clinical Specimens

Comparison of the Lowenstein-Jensen Medium, the Middlebrook 7H10 Medium and MB/BacT for the Isolation of Mycobacterium Tuberculosis (MTB) from Clinical Specimens

Drug Susceptibility Testing of Mycobacterium tuberculosis by a Nitrate Reductase Assay Applied Directly on Microscopy-Positive Sputum Samples

A study on pattern of initial and acquired drug resistance for isoniazid and rifampicin in A.F.B. positive sputum smears of pulmonary tuberculosis patients at a Medical College in North Eastern Uttar Pradesh, India

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