Evaluation of the BAX® system for the detection of Salmonella spp. in naturally contaminated chicken meat

Frausto, Harissa Silvério El Ghoz; Alves, Juliane; Oliveira, Tereza Cristina Rocha Moreira de

doi:10.1590/s0101-20612013005000056

Cited by 5 publications

(2 citation statements)

References 16 publications

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“…Other methodologies have been developed to reduce the possibility of contamination or error during PCR, such as the USDA‐FSIS‐approved BAX® (Wasilenko and others ) system, which consists of only 1 inoculation step. (Bailey ; Shearer and others ; Frausto and others ).…”

Section: Molecular Methods For Stec Identificationmentioning

confidence: 95%

Shiga‐toxin Producing Escherichia coli: Pathogenicity, Supershedding, Diagnostic Methods, Occurrence, and Foodborne Outbreaks

Castro

Carvalho

Conté-Júnior

et al. 2017

Comp Rev Food Sci Food Safe

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Historically, Escherichia coli is among the most studied organisms and serves as the basis for understanding many fundamental biochemical and genetic concepts. In addition, it displays 9 pathogenesis groups, with the Shiga toxin-producing (STEC) group being the main representative regarding foodborne pathogenesis. Its typical characteristic is the presence of 2 distinct toxins and variants: stx1 (stx1a, stx1c, and stx1d), and stx2 (stx2a, stx2b, stx2c, stx2d, stx2e, stx2f, and stx2g). The main challenge regarding the study of E. coli is the standardization of a high sensitivity method including all pathotypes, that allows for enrichment of STEC cells and a decrease of background microbiota. The ability of some E. coli cells belonging to other pathogenic groups, such as O104:H4, to acquire genes unique to the STEC group, increases the pathogenic power and the risk of new outbreaks related to these bacteria. In addition, animals with a high concentration of pathogenic E. coli cells present in feces (above 10 4 CFU/g), designated as supershedding animals, may be the primary transmission factor among ruminants. Therefore, the purpose of this review is to address pathogenicity factors and the importance of supershedding animals in the transmission of this pathogen, discussing the main methods currently applied, to focus on the occurrence of STEC in beef.

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Section: Molecular Methods For Stec Identificationmentioning

confidence: 95%

Shiga‐toxin Producing Escherichia coli: Pathogenicity, Supershedding, Diagnostic Methods, Occurrence, and Foodborne Outbreaks

Castro

Carvalho

Conté-Júnior

et al. 2017

Comp Rev Food Sci Food Safe

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“…Rapid molecular-based methodologies, such as real-time polymerase chain reaction (qPCR), are alternatives that can improve the accuracy and efficiency of SE testing and confirmation [13][14][15][16][17]. Unlike the traditional Salmonella culture-based approaches, several investigations have shown that qPCR methods provide superior efficiency and specificity in detecting SE in a variety of food matrices, including shell eggs [16,18,19].…”

Section: Introductionmentioning

confidence: 99%

A Real-Time PCR Approach for Rapid Detection of Viable Salmonella Enteritidis in Shell Eggs

Chan¹,

Liau²,

Low³

et al. 2023

Microorganisms

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Rapid and robust detection assays for Salmonella Enteritidis (SE) in shell eggs are essential to enable a quick testing turnaround time (TAT) at the earliest checkpoint and to ensure effective food safety control. Real-time polymerase chain reaction (qPCR) assays provide a workaround for the protracted lead times associated with conventional Salmonella diagnostic testing. However, DNA-based analysis cannot reliably discriminate between signals from viable and dead bacteria. We developed a strategy based on an SE qPCR assay that can be integrated into system testing to accelerate the detection of viable SE in egg-enriched cultures and verify the yielded SE isolates. The specificity of the assay was evaluated against 89 Salmonella strains, and SE was accurately identified in every instance. To define the indicator for a viable bacteria readout, viable or heat-inactivated SE were spiked into shell egg contents to generate post-enriched, artificially contaminated cultures to establish the quantification cycle (Cq) for viable SE. Our study has demonstrated that this technique could potentially be applied to accurately identify viable SE during the screening stage of naturally contaminated shell eggs following enrichment to provide an early alert, and that it consistently identified the serotypes of SE isolates in a shorter time than conventional testing.

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Shiga toxin-producing Escherichia coli in food: Incidence, ecology, and detection strategies

et al. 2016

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Evaluation of the BAX® system for the detection of Salmonella spp. in naturally contaminated chicken meat

Cited by 5 publications

References 16 publications

Shiga‐toxin Producing Escherichia coli: Pathogenicity, Supershedding, Diagnostic Methods, Occurrence, and Foodborne Outbreaks

Shiga‐toxin Producing Escherichia coli: Pathogenicity, Supershedding, Diagnostic Methods, Occurrence, and Foodborne Outbreaks

A Real-Time PCR Approach for Rapid Detection of Viable Salmonella Enteritidis in Shell Eggs

Shiga toxin-producing Escherichia coli in food: Incidence, ecology, and detection strategies

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