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Purpose – The aim of this study was to serotype strains of Salmonella isolated from poultry and raw poultry meat from 2006 to 2010 and to correlate the results with data from the Health Department of Parana State. Design/methodology/approach – A total of 1.165 Salmonella spp. strains isolated from commercial broiler breeders and laying hens (cloacal swabs, dragging swabs and faeces) and from one-day-old chicks (liver, spleen and cecum) and 310 strains isolated from raw chicken meat were serotyped between 2006 and 2010. Findings – The results showed a decline in the isolation of S. Enteritidis (SE) of poultry and chicken meat in the studied period. The most prevalent serovar isolated from raw chicken meat was Minnesota (n=73; 23.02%), followed by Mbandaka (n=33; 10.41%) and Enteritidis (n=30; 9.46%). The decrease in frequency of isolation of SE may be related to the broiler breeder hens vaccination and to the National Program for the Reduction of Pathogens of the Brazilian Agriculture Ministry and Food Supply, established in 2003. Originality/value – The results reinforce the importance of improving bio-safety programs for the control and eradication of Salmonella spp. in the poultry industry.