Evaluation of the BBL Crystal MRSA ID System for detection of oxacillin resistance in Staphylococcus aureus.

Kampf, Günter; Lecke, Christoph; Cimbal, Ann-Katrin; Weist, Klaus; Rüden, H.

doi:10.1136/jcp.52.3.225

Cited by 10 publications

(5 citation statements)

References 19 publications

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“…As multiresistant nosocomial pathogens of high epidemiological interest (i.e., vancomycin-resistant S. aureus ) were not kept, further diagnostic validation could not be conducted. Differences in the sensitivity and specificity of laboratory diagnostic techniques have been described, which might impact on stain identification [23]. …”

Section: Discussionmentioning

confidence: 99%

Prevalence of nosocomial infections and anti-infective therapy in Benin: results of the first nationwide survey in 2012

Ahoyo

Bankolè

Adéoti³

et al. 2014

Antimicrob Resist Infect Control

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BackgroundData on nosocomial infections in hospitals in low-income countries are scarce and often inconsistent. The objectives of this study were to estimate the prevalence of nosocomial infections and antimicrobial drug use in Benin hospitals.MethodsAll hospitals were invited to participate in the first national point prevalence study conducted between 10–26 October 2012 using the protocol developed by the “Hospitals in Europe Link for Infection Control through Surveillance” (HELICS) project. Infection prevalence rates and the proportion of infected patients and exposure to antimicrobials were assessed.ResultsOverall, 87% (39/45) of hospitals participated. Of 3130 inpatients surveyed, 972 nosocomial infections were identified among 597 patients, representing an overall prevalence of infected patients of 19.1%. The most frequent infections were related to the urinary tract (48.2%), vascular catheter use (34.7%), and surgical site (24.7%). 64.6% of patients surveyed were treated with antibiotics, including a significant proportion (30%) of non-infected patients and a high proportion of self-medication (40.8%). Resistance of leading nosocomial pathogens to antimicrobials included methicillin-resistance (52.5%) among Staphylococcus aureus, vancomycin resistance among enterococci (67.5%), cefotaxime resistance among Escherichia coli (67.6%), and ceftazidime resistance among Acinetobacter baumannii (100%) and Pseudomonas aeruginosa (68.2%).ConclusionsBenin has high nosocomial infection rates and calls for the implementation of new national infection control policies. Patient safety education and training of all individuals involved in healthcare delivery will be critical to highlight awareness of the burden of disease. The high use of antimicrobials needs to be addressed, particularly their indiscriminate use in non-infected patients.

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Section: Discussionmentioning

confidence: 99%

Prevalence of nosocomial infections and anti-infective therapy in Benin: results of the first nationwide survey in 2012

Ahoyo

Bankolè

Adéoti³

et al. 2014

Antimicrob Resist Infect Control

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“…Table 3 shows the basic techniques, steps of performance, need for using the patients' samples directly or the primary culture, the equipment required, and the time needed to obtain results of the current methods used for identification of MRSA/MSSA and our new method. [25][26][27][28][29][30][31][32][33] Our method is based on the growth rate difference of S. aureus in two environments, with or without oxacillin. MRSA and MSSA grow at similar rates in normal media, however MSSA grows significantly slower than MRSA in presence of oxacillin.…”

Section: Discussionmentioning

confidence: 99%

An Easy-to-Use, Rapid and Inexpensive Method to Determine Methicillin Resistance In Staphylococcus aureus

Göçmen¹,

Çağlayan²,

Azap³

2016

J Clin Exp Invest

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Objective:We aimed to report a new turbidimetric method to identify methicillin resistance in S. aureus strains just two hours after identification of the microorganism, and to analyze diagnostic and discrimination abilities of this new method. Methods: A total of 319 S. aureus isolates were included. Identification of bacteria was done by the colony morphology, and conventional biochemical methods. The methicillin resistance of the S. aureus strains was studied as indicated in Clinical Laboratory Standards Institute 2009. The turbidimetric method we developed is based on different growth rates of S. aureus in two media, with or without oxacillin. The growth rates of MRSA and MSSA are similar in normal media, however the MRSA grows significantly faster in the media containing oxacillin. Therefore, after 2 hours of incubation, the difference of turbidity produced by bacteria is less in MRSA, and more in MSSA. The absorbance of the microplates were measured before incubation, and at 2nd and 3rd hours of incubation. The "absorbance rate" was calculated for each bacteria and the bacteria were classified as MRSA or MSSA based on the absorbance rate. Results: All MRSA and MSSA strains were correctly discriminated via our turbidimetric method, when an absorbance rate of 1.900 was taken as cut-off value. The new method could diagnose MRSA with 100% specificity and 100% sensitivity in just two hours. Conclusion:The turbidimetric method is a rapid, easy and cheap method that does not require any specific equipment. It can be easily performed in every microbiology laboratory.

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“…Differences in the sensitivity and specificity have been described [28-30] which may have an impact on the identification of a MRSA patient. But it is unlikely to have an impact on the result of the antiseptic treatment because the method would have been the same before and after treatment of the same patient.…”

Section: Discussionmentioning

confidence: 99%

Eradication of methicillin-resistant Staphylococcus aureus with an antiseptic soap and nasal mupirocin among colonized patients – an open uncontrolled clinical trial

Kampf

Krämer

2004

Ann Clin Microbiol Antimicrob

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Evaluation of the BBL Crystal MRSA ID System for detection of oxacillin resistance in Staphylococcus aureus.

Abstract: (J Clin Pathol 1999;52:225-227)

Cited by 10 publications

References 19 publications

Prevalence of nosocomial infections and anti-infective therapy in Benin: results of the first nationwide survey in 2012

Prevalence of nosocomial infections and anti-infective therapy in Benin: results of the first nationwide survey in 2012

An Easy-to-Use, Rapid and Inexpensive Method to Determine Methicillin Resistance In Staphylococcus aureus

Eradication of methicillin-resistant Staphylococcus aureus with an antiseptic soap and nasal mupirocin among colonized patients – an open uncontrolled clinical trial

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