Evaluation of the biofilm forming ability and its associated genes in Staphylococcus species isolates from bovine mastitis in Argentinean dairy farms

Felipe, Verónica; Morgante, Carolina; Somale, Paola Sofía; Varroni, Florencia; Zingaretti, Laura M.; Bachetti, Romina; Correa, Silvia G.; Porporatto, Carina

doi:10.1016/j.micpath.2017.01.047

Cited by 57 publications

(56 citation statements)

References 41 publications

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“…This is consistent with previous results reporting that S. aureus strains causing intramammary infection in dairy cattle were determined as biofilm former strains (Felipe et al . ).…”

Section: Resultsmentioning

confidence: 97%

“…; Felipe et al . ). Polysaccharide intracellular adhesin (PIA) and microbial surface component recognising adhesive matrix molecules (MSCRAMMs) have played important roles in the adhesion and biofilm formation of staphylococci on food processing surfaces.…”

Section: Introductionmentioning

confidence: 97%

“…In addition to the Ica locus, fnbA (fibronectin‐binding proteins) and clfA (clumping factor) are significant genes that encode MSCRAMMs for biofilm formation (Felipe et al . ). Some S. aureus strains also produce an extracellular thermostable nuclease encoded by the nuc gene that reduces both DNA and RNA (Wang et al .…”

Section: Introductionmentioning

confidence: 97%

“…Biofilm is an adhesive mass of bacteria enclosed by an extracellular polymeric matrix (Periasamy et al 2012). It has been reported that in the case of bovine mastitis caused by S. aureus and other staphylococci, the ability to form biofilm is one of the virulence factors for the mammary gland epithelium leading to infections in animals (Felipe et al 2017). It is also known that biofilm-associated bacteria are more resistant to disinfectants or antimicrobial agents than their planktonic counterparts (Bridier et al 2015).…”

Section: Introductionmentioning

confidence: 99%

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Biofilm formation by Staphylococcus aureus strains and their control by selected phytochemicals

Ünlü

Şar

Seker

et al. 2018

Int J of Dairy Tech

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Biofilm formation by 30 Staphylococcus aureus dairy isolates and their control by phytochemicals were investigated. The majority of strains were biofilm positive by phenotypic analysis. The nuc and icaA genes were present in 30 and 27 strains, respectively. In addition, 13 strains were positive for all nuc, clfA, fnbA and icaA genes. The antimicrobial and antibiofilm activities of citral, cinnamaldehyde, eugenol, farnesol, limonene and terpinen‐4‐ol were also evaluated for seven strains. It was shown that the use of farnesol, cinnamaldehyde or terpinen‐4‐ol at a concentration of 2 mg/mL could be at least 80% effective on S. aureus strains and their biofilms.

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“…This is consistent with previous results reporting that S. aureus strains causing intramammary infection in dairy cattle were determined as biofilm former strains (Felipe et al . ).…”

Section: Resultsmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Biofilm formation by Staphylococcus aureus strains and their control by selected phytochemicals

Ünlü

Şar

Seker

et al. 2018

Int J of Dairy Tech

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“…In S. aureus , the genetic polymorphism of agrB, agrC, and agrD is determined by 4 allelic groups (from I to IV). Some of the virulence factors are located on the so-called S. aureus pathogenicity islands, which are mobile elements and give staphylococcal cells enormous plasticity and variability [17]. …”

Section: Introductionmentioning

confidence: 99%

<b><i>Staphylococcus aureus</i></b> and Host Immunity in Recurrent Furunculosis

Nowicka

Grywalska

2019

Dermatology

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Staphylococcus aureus is one of the severest and most persistent bacterial pathogens. The most frequent S. aureus infections include impetigo, folliculitis, furuncles, furunculosis, abscesses, hidradenitis suppurativa, and mastitis. S. aureus produces a great variety of cellular and extracellular factors responsible for its invasiveness and ability to cause pathological lesions. Their expression depends on the growth phase, environmental factors, and location of the infection. Susceptibility to staphylococcal infections is rooted in multiple mechanisms of host immune responses and reactions to bacterial colonization. Immunological and inflammatory processes of chronic furunculosis are based on the pathogenicity of S. aureus as well as innate and acquired immunity. In-depth knowledge about them may help to discover the whole pathomechanism of the disease and to develop effective therapeutic options. In this review, we focus on the S. aureus-host immune interactions in the pathogenesis of recurrent furunculosis according to the most recent experimental and clinical findings.

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Artesunate inhibits Staphylococcus aureus biofilm formation by reducing alpha-toxin synthesis

Yan

Lai

et al. 2020

Arch Microbiol

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Staphylococcus aureus is one of the most common pathogens in bacterial biofilm infections. Antibiotic treatment for infection caused by S. aureus biofilms is challenging, and few effective strategies have been developed to combat these infections. The aim of this study was to investigate the effect and possible mechanisms of artesunate on the biofilm formation of S. aureus. Bacterial growth curves were determined by a microtiter plate. Biofilm formation was determined by the crystal violet staining method and confocal laser scanning microscopy. Bacterial adhesion was assayed by the colony-counting method. The expression of virulence and adhesion genes was determined by real-time PCR. The hemolytic activity and expression of ɑ-hemolysin were analyzed using rabbit erythrocytes and Western blotting. The results showed that artesunate could significantly inhibit the biofilm formation of S. aureus in a dose-dependent manner. Artesunate could also inhibit bacterial adhesion and the expression of hla, RNAIII and agrA as well as ɑ-hemolysin production. The effect of artesunate on adhesion genes (clfA, clfB, fnbA, fnbB) had strain specificity, but it did not affect the expression of ica genes. The results indicated that artesunate might inhibit ɑ-hemolysin synthesis by the agr system, which inhibits biofilm formation.

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Evaluation of the biofilm forming ability and its associated genes in Staphylococcus species isolates from bovine mastitis in Argentinean dairy farms

Cited by 57 publications

References 41 publications

Biofilm formation by Staphylococcus aureus strains and their control by selected phytochemicals

Biofilm formation by Staphylococcus aureus strains and their control by selected phytochemicals

<b><i>Staphylococcus aureus</i></b> and Host Immunity in Recurrent Furunculosis

Artesunate inhibits Staphylococcus aureus biofilm formation by reducing alpha-toxin synthesis

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