Evaluation of the biological behaviour of various dental implant abutment materials on attachment and viability of human gingival fibroblasts

Pandoleon, Panagiotis; Bakopoulou, Athina; Papadopoulou, Lambrini; Koidis, Petros

doi:10.1016/j.dental.2019.04.010

Cited by 44 publications

(38 citation statements)

References 46 publications

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“…However, this result was considered a false positive effect and could be caused by the metallic particles released into the extraction medium and their further interaction with the MTT reagent. This type of interaction has been previously identified in several in vitro studies [31][32][33]. Still, the P2 and P3 samples did not interfere with the MTT technique, as their corresponding micro-implants did not release metallic microparticles in the extraction medium, as could be easily observed from Figure 3.…”

Section: Cell Viability Assay By Means Of Mtt Analysissupporting

confidence: 71%

Comparative Toxicological In Vitro and In Ovo Screening of Different Orthodontic Implants Currently Used in Dentistry

et al. 2020

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Selecting the most biocompatible orthodontic implant available on the market may be a major challenge, given the wide array of orthodontic devices currently available on the market. The latest scientific data have suggested that in vitro evaluations using oral cell lines provide reliable data regarding the toxicity of residual particles released by different types of orthodontic devices. In this regard, the in vitro biocompatibility of three different commercially available implants (stainless steel and titanium-based implants) was assessed. Methods: As an in vitro model, human gingival fibroblasts (HGFs) were employed to evaluate the cellular morphology, cell viability, and cytotoxicity by means of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and lactate dehydrogenase (LDH) assays at 24 h and 72 h post-exposure to test implants. Results: The results correlate the composition and topography of the implant surface with biological experimental evaluations related to directly affected cells (gingival fibroblasts) and toxicological results on blood vessels (hen’s egg test-chorioallantoic membrane (HET-CAM) assay). The stainless steel implant exhibits a relative cytotoxicity against HGF cells, while the other two samples induced no significant alterations of HGF cells. Conclusion: Among the three test orthodontic implants, the stainless steel implant induced slight cytotoxic effects, thus increased vigilance is required in their clinical use, especially in patients with high sensitivity to nickel.

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Section: Cell Viability Assay By Means Of Mtt Analysissupporting

confidence: 71%

Comparative Toxicological In Vitro and In Ovo Screening of Different Orthodontic Implants Currently Used in Dentistry

et al. 2020

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“…It was observed that the cell viability percentage of HGF cells that were treated with PLGA sample was almost 115%, indicating that the sample elicited a proliferative effect. This effect could have been produced by an interaction of its acidic groups with the MTT reagent, and thus it can be considered a false positive result; similar interactions were identified in other in vitro studies [14][15][16]. HGF cells viability after treatment with PU sample was almost 90 % and this value is specific to a non-cytotoxic sample.…”

Section: Figuresupporting

confidence: 52%

Synthesis and Comparative Characterization of Different Microparticles used as Biomaterials in Dentistry

Tofan¹,

Olteanu²,

Szuhanek³

et al. 2021

Mater. Plast.

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A biomaterial must be biologically compatible, mechanical, functional, corrosion resistant and easily adapt to clinical and laboratory technologies. Dental biomaterials are materials used to replace a part of a living system or to work closely with living tissue. Many scientific articles present different polymeric biocomposites with possible application in dentistry and this is a proof of the opportunity of a research in a field in full ascent and with great availability in the promotion of materials destined to �work under biological constraint� and which must also meet the functional requirements of a dental implant. The objectives of this research were to obtain and to comparatively evaluate different polymeric microparticles that can be used in dentistry. The samples based on poly(lactic-co-glycolic acid) and respectively polyurethane microparticles were characterized by pH and Zetasizer measurements, and in vitro cytotoxicity assays. The results indicate the obtaining of particles with a neutral pH, medium homogeneity, and with different tendencies to form agglomerations. Their low cytotoxicity, tested on the primary human gingival fibroblasts by MTT and LDH techniques, indicates that these microparticles are safe to be tested in further clinical evaluations.

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“…Some vitro studies suggested that during simulated aging process there is decrease in fracture toughness (50%) of zirconia in humid environment and it also effect gingival cell attachment and proliferation properties. 19,[25][26][27] In the present study, zirconia abutments used were of metallic internal implant-abutment connection. Presently various new designs of zirconia abutments are available with different implant abutment connections.…”

Section: Discussionmentioning

confidence: 99%

Effect of different abutment materials (zirconia or titanium) on the crestal bone height in 1 year

Bharate

Kumar

Koli

et al. 2020

Journal of Oral Biology and Craniofacial Research

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Evaluation of the biological behaviour of various dental implant abutment materials on attachment and viability of human gingival fibroblasts

Cited by 44 publications

References 46 publications

Comparative Toxicological In Vitro and In Ovo Screening of Different Orthodontic Implants Currently Used in Dentistry

Comparative Toxicological In Vitro and In Ovo Screening of Different Orthodontic Implants Currently Used in Dentistry

Synthesis and Comparative Characterization of Different Microparticles used as Biomaterials in Dentistry

Effect of different abutment materials (zirconia or titanium) on the crestal bone height in 1 year

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